초록 ▼

Ⅲ. 연구개발의 내용 및 범위
본 연구에서는 감귤바이오겔 이용 융합기술 및 맞춤형 겔 개발을 위하여 감귤 바이오겔을 미세입자 파우더형으로 개발하고자 하였다. 감귤 바이오겔을 분해 할 수 있는 효소를 선별하고, 선별된 효소를 이용하여 감귤 바이오겔을 미세입자 파우더로 만들어 다양한 형태의 향장품을 개발하고자 하였다.
또한 천연물 유래 감귤 바이오겔을 이용한 창상치유의 효능과 인공피부로서의 임상검증을 위하여 감귤 바이오겔의 창상 치료용 bio material 원료특성과 세포독성시험 및 물리학적 특성을 조사하였다. 창상치료용

Ⅲ. 연구개발의 내용 및 범위
본 연구에서는 감귤바이오겔 이용 융합기술 및 맞춤형 겔 개발을 위하여 감귤 바이오겔을 미세입자 파우더형으로 개발하고자 하였다. 감귤 바이오겔을 분해 할 수 있는 효소를 선별하고, 선별된 효소를 이용하여 감귤 바이오겔을 미세입자 파우더로 만들어 다양한 형태의 향장품을 개발하고자 하였다.
또한 천연물 유래 감귤 바이오겔을 이용한 창상치유의 효능과 인공피부로서의 임상검증을 위하여 감귤 바이오겔의 창상 치료용 bio material 원료특성과 세포독성시험 및 물리학적 특성을 조사하였다. 창상치료용 Bio-gel Foam드레싱의 제작 및 효능 실험을
위해 임상 적용가능한 창상치료용 Bio-gel Foam드레싱을 제작하여 Bio-gel Foam드레싱의 창상 치유 효능을 측정하였다.
감귤 발효겔의 방사선 융함기술을 활용한 하이드로겔 제조 기술 개발을 위해 감귤발효겔을 이용한 상처 치료용 하이드로겔과 천연고분자와 감귤 발효겔을 합성한 하이드로겔을 개발하여 세포독성, 생체/조직적합성을 측정하여 생체적합성 고분자/감귤 발효겔 기반 방사선 이용 상처치료용 기능성소재 제조 기술을 개발하고자 하였다.

Abstract ▼

Strains of cellulose producing bacteria belong to Gluconacetobacter sp. gel_SEA623-2 were isolated from Citrus fruit juice fungus and then strain go to RDA-Genebank Information Center. Gel production speed of SEA623-2 strain was very fast compared to other strains as formed from cultivation 2nd dist

Strains of cellulose producing bacteria belong to Gluconacetobacter sp. gel_SEA623-2 were isolated from Citrus fruit juice fungus and then strain go to RDA-Genebank Information Center. Gel production speed of SEA623-2 strain was very fast compared to other strains as formed from cultivation 2nd distinctively. Hardness of produced SEA623-2 gel was 10~15N/cm2 which is suitable applies with medical and cosmetics materials.
Cellulose, chitin, chitosan and hyaluronic acid are well known as polysaccharides. These polysaccharides have many effects on cell growth and differentiation. Cell activation increases with increasing the polysaccharides concentration. In particular,
bacterial cellulose (BC) is generated from citrus gel by Gluconacetobacter sp. SEA623-2. BC gel has good properties such as high-burst pressure, high-water content and the ultrafine highly nanofibrous structure, foam of mimic natural extracellular matrix (ECM) for tissue engineering.
In this research project, biofunctional hydrogels and foams containing the BC were developed by using irradiation technique for wound dressing. Irradiation has been recognized as a highly suitable tool to aid in the formation of the desired hydrogels and solves the problem of sterilization of products and a few cases llows to establish a more simple and compact technology than a chemical graft method. The characterization of the BC based hydrogels and foams was performed by measuring of gel fraction, swelling ratio, and gel strength as a function of the concentrations of biocompatible materials and the BC. In addition, the wound healing has been evaluated by the cytotoxicity and animal experiment. The results showed that these BC hydrogels and foams have good mechanical properties and an excellent wound healing. Therefore these findings indicate that the BC hydrogels
and foams can be used as a potential wound dressing materials.
In recent years, natural polymers such as cellulose, alginate and chitosan have been used worldwide as biomedical materials and devices, as they offer more advantages over synthetic polymers. The aim of this study was to clarify the usefulness of bacterial cellulose (BC) for use as a dressing and scaffold material. To evaluate the wound healing effects of BC, three full-thickness skin defects were made on the backs of each rat. Three wounds on the same back of rats were treated with other dressing materials, namely, Vaseline gauze (Con), Algisite M^Ⓡ (Alg) and BC. BC showed more rapid wound healing compared with Con. On day 14 after skin excision, BC showed greater decrease in wound size compared with Con (33% versus 7·2%). The wound healing effects were also substantiated by the histological findings (greater reduction in inflammation and rapid collagen deposition as well as neovascularisation) and western blotting (decreased expression of vascular endothelial growth factor and transforming growth factor-β1 in BC on day 14 after skin excision, unlike Con). For evaluating the biodegradability and toxicity of BC, we divided the rats into two groups (the non implanted- and implanted group). In the implanted group, we implanted the film type of BC in the backs of rats. In the non implanted group, however, we do not implant the film type of BC in the backs of the rats. BC was found to be biodegradable and non toxic. On day 3, the BC film was visible under the subcutaneous tissue; however, after 4 weeks, no remnants of the film were visible under the subcutaneous tissue.
Furthermore, there was no evidence of BC-induced toxicity. To evaluate the wound healing effects of foam dressing contain BC, this experiments were performed in the same manner as previous wound experiments. Back of rats were treated with three
dressing materials (group A contain chitosan, β-glucan and BC, group B contain chitosan and β-glucan, group C contian BC). Group B and C showed similar wound healing effect, however group A was most rapidly wound healing. This study showed that, in addition to having wound healing effects, BC is biodegradable and non toxic and can, therefore, be used as a dressing and scaffold material.

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 요약문 ... 3
• SUMMARY ... 6
• 목차 ... 8
• 제1장 서론 ... 9
• 제2장 국내외 기술개발 현황 ... 11
• 제1절 국내 기술개발 현황 ... 11
• 제2절 국외 기술개발 현황 ... 11
• 제3절 국내외 연구현황 비교 및 필요 연구분야 ... 13
• 제3장 연구개발수행 내용 및 결과 ... 15
• 제1절 감귤 바이오겔 이용 융합 기술 개발 및 맞춤형 겔 개발 ... 15
• 제2절 감귤 바이오겔을 이용한 창상치유의 효능과 인공피부로서의 임상 검증 ... 18
• 제3절 감귤 발효겔의 방사선 융합기술을 활용한 하이드로겔 제조기술 개발 ... 40
• 제4장 연구개발목표 달성도 및 대외기여도 ... 51
• 제1절 목표대비 달성도 ... 51
• 제2절 정량적 성과 ... 52
• 제5장 연구개발결과의 활용계획 ... 55
• 제6장 연구개발과정에서 수집한 해외과학기술정보 ... 56
• 제7장 기타 중요 변동사항 ... 57
• 제8장 국가과학기술종합정보시스템에 등록한 연구장비 현황 ... 58
• 제9장 참고문헌 ... 59
• <참고> 주요 연구성과 요약 ... 62