양식산 넙치 체색이상발현 원인구명 및 체색발현 제어기술 개발 Study of the causes of malpigmentation and development of pigmentation control technology in cultured olive flounder원문보기
Ⅲ. 연구개발 내용 및 범위 1. 양식산 넙치의 유안측 백색증 발현에 미치는 환경인자들의 영향 2. 양식산 넙치의 성장단계별 무안측 흑화의 발현 양상 3. 양식산 흑화 넙치의 생리학적 특성 4. 넙치의 무안측 흑화 발현에 있어 분자내분비적 관련성 4-1. MCH 관련성 4-2. POMC 관련성 5. 유전육종학적 관련성 5-1. 가계군집 간 관련성 5-2. 유전적 표현인자 6. 무안측 흑화 발현에 있어 서식 환경의 영향 6-1. 사육 밀도의 영향 6-2. 수조
Ⅲ. 연구개발 내용 및 범위 1. 양식산 넙치의 유안측 백색증 발현에 미치는 환경인자들의 영향 2. 양식산 넙치의 성장단계별 무안측 흑화의 발현 양상 3. 양식산 흑화 넙치의 생리학적 특성 4. 넙치의 무안측 흑화 발현에 있어 분자내분비적 관련성 4-1. MCH 관련성 4-2. POMC 관련성 5. 유전육종학적 관련성 5-1. 가계군집 간 관련성 5-2. 유전적 표현인자 6. 무안측 흑화 발현에 있어 서식 환경의 영향 6-1. 사육 밀도의 영향 6-2. 수조 색상의 영향 6-3. 수조 바닥 재질의 영향 6-4. 잠입기질 색상의 영향 6-5. 잠입기질 제거와 및 월동기 동면 효과 7. 산업화 가능성 실험 7-2. 대량생산 예비실험 7-1. 가자미목 어류 적용 실험
Abstract▼
2.1. Effects of environment factors on the occurrence of pseudo-albinism on the ocular side in cultured flounder, P. olivaceus We examined the cause of albinism in a hatchery population of flounder in terms of environmental and nutritional factors, including the effects of light intensity (130~12,30
2.1. Effects of environment factors on the occurrence of pseudo-albinism on the ocular side in cultured flounder, P. olivaceus We examined the cause of albinism in a hatchery population of flounder in terms of environmental and nutritional factors, including the effects of light intensity (130~12,300 lux), photoperiod (12L/12D or 24L/0D), tank substrate(concrete or fiber-reinforced polymer), tank color (white or dark green) and supplement with enriched live food. We also compared the growth of normal and albino fry under both 12L/12D and 24L/0D. After rearing the albino fry for 2 years after their identification and classification, we investigated whether the skin pigmentation pattern on the ocular side normalized. Light intensity did not play a critical role in the development of normal pigmentation or albinism in the flounder. By contrast, the photoperiod was a weak inducer of albinism in the flounder. Tank substrate and color also affected the hypomelanosis on the ocular side of the flounder fry. The choice and supplementation of enriched live foods could drastically reduce the incidence of albinos in hatcheries. On comparing the growth of normal and albino fry, while there was no difference between the groups under 24L/0D, the growth of the albino flounder was slower under 12L/12D. Although coloration resulting from xanthophore and melanophore, but not iridophores, occurred on the ocular side, evidence of albinism remained on the ocular side of flounders. 2.2. Change of abnormal pigmentation on blind side of cultured flounder, P. olivaceus according to growth stage In the present study, to understand a mechanism of abnormal pigmentation (hypermelanosis) in blind side of flounder, P. olivaceus, we investigated an date that blind side staining visible to ordinary sight is initially observed, and measured an individual staining spot number, staining rate and an occurrence frequence in crowd that are changed according to growth. The present experiment was initiated at 24-day after hatching (dah), just after metamorphosis, in which fish mean size is total length [TL] 12.4±0.2 mm and body weight [BW] 19.9±0.7 mg, and was finalized at 94-dah (TL 72.0±0.6 mm, BW 3,912.3±119.4 mg). The experimental animals were reared at 20±0.5℃ in water temperate and 30.9±0.1 in salinity. The experimental animals were feed with rotifer, Artemia nauplii and commercial food according growth stage. As results, the first staining spot in microscopy level was found at 38-day after hatching(TL 18.3±0.4 mm, BW 57.6±4.1 mg), but the first staining spot observed by naked eye was found 52-day after hatching (TL 37.6±0.5 mm, BW 534.2±30.9 mg). The staining spot sharply increased from 31-dah to 80-dah, and the staining rate sharply increased from 66-dah to 94-dah. The number of staining fish per group was increased from 45-dah to 73-dah, and then was stabilized to 94-dah. Therefore, these results indicated that the hypermelanosis in blind side of the flounder, P. olivaceus was a phenomenon that pigment cells is abnormally differentiated on the blind side just after metamorphosis in artificial culture system, and the staining spot constituted with the pigment cells was increased according to growth.
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