초록 ▼

▶ 연구목적 :
○대사성질환 관련물질 고함유 농산물자원(곡류, 과채류)선발 및 DB구축
○대사성질환 조절물질 물질분석, 효능검정(항비만, 항당뇨 합병증) 및 최적유전자원 선발 및 최적유전자원의 대사성질환 억제 조절물질의 기전연구
○대사성질환 관련물질 Scale up 기술개발
▶ 주요연구내용 :
○ 1세부과제명 : 대사성질환 예방물질 고함유 농산물자원발굴 및 대량생산기술 개발
- 율무 유전자원에서 Uronic acid, Ferulic acid 고함유 자원선발
- 도정후 나오는 율무종피에Uronic

▶ 연구목적 :
○대사성질환 관련물질 고함유 농산물자원(곡류, 과채류)선발 및 DB구축
○대사성질환 조절물질 물질분석, 효능검정(항비만, 항당뇨 합병증) 및 최적유전자원 선발 및 최적유전자원의 대사성질환 억제 조절물질의 기전연구
○대사성질환 관련물질 Scale up 기술개발
▶ 주요연구내용 :
○ 1세부과제명 : 대사성질환 예방물질 고함유 농산물자원발굴 및 대량생산기술 개발
- 율무 유전자원에서 Uronic acid, Ferulic acid 고함유 자원선발
- 도정후 나오는 율무종피에Uronic acid, Ferulic acid 함량이 종실보다 많아 부산물 산업화 이용 가능
- 토마토 유전자원에서 알파토마틴 트리고넬린 고함유 유전자원 선발 및 DB구축
- 토마토 부산물인 녹색토마토의 크기별 알파토마틴, 트리고넬린 함량규명[품종별과실크기별 함량변이 폭이 크고 작을수록 성분 고함유
- 녹색토마토의 알파토마틴 증진을 위한 LED광 효과(적색 청색 혼합광)와 트리고 넬린 증진을 위한 미네랄효과(Mg, Cu, Zn혼합)규명
- 녹색토마토 활용 맛있는 피클제조
○ 2세부과제명 : 곡류 및 과채류의 대사성질환 조절을 위한 생리활성검색
- 지방조직 및 지방간의 무게와 총콜레스테롤 감소(녹색토마토가 효능 뛰어남)
- 비만유도 동물실험에서 체중감소(녹색토마토가 효능 뛰어남)
- 신경성장인자의 분비를 유도하고 신경성장인자 유사작용을 지님(녹색 토마토가 효능 뛰어남)
- 청력역치의 상승을 효과적으로 억제하며 청신경전달속도를 증진시킴(녹토 적토 뛰어난 효능 나타냄)
- 율무 가지에서도 지방분화세포 억제 작용이 뛰어나고 부작용이 없음

Abstract ▼

IV. Results
1. Scale up Production Technique and Selection of Best Genetic Resource with Regulatory Activity for Metabolic Disease in Crops
There are great variation between 63 varieties in contents of uronic acid and ferulic acid. Among 63 varieties in adly(Coix lachryma-jobi var. ma-yuen), 3

IV. Results
1. Scale up Production Technique and Selection of Best Genetic Resource with Regulatory Activity for Metabolic Disease in Crops
There are great variation between 63 varieties in contents of uronic acid and ferulic acid. Among 63 varieties in adly(Coix lachryma-jobi var. ma-yuen), 3 varieties have the content of uronic acid over 5000(ug/g) and 2 varieties have the content of ferulic acid over 2000(ug/g). The everage content of uronic acid and ferulic acid was shown in seed coat higher than in grain without seed coat in adly. The seed coat in adly is very good source for functional food materials. 138 varieties in tomatos(Solanumlycopersicum), those are received from national agrobiodiversity center in RDA, were cultivated in green house in 2010. There are great variation between 138 varieties in contents of tomatine and trigonelline in green tomato fruits(9-12g size). Among 138 varieties in green tomato, IT NO. 207219 has the greatest content of ɑ-tomatine with 154.50ppm than any other varieties and IT NO. 199429 has the greatest content of trigonelline with 421.89ppm than any other varieties . In order to enhance the content of tomatine and trigonelline in green tomato fruits, the scale up production technique(fruits sizes, treatments of LED, UV and mineals) were performed. The effect of scale up production technique were very different between 2 experimental varieties. Smaller the size of tomato fruits, much higher the content of ɑ-tomatine and trigonelline. The small size of green tomato(by-product) is very good source for functional food materials. The effect of LED treatment on ɑ-tomatine was shown in treatment with red light(100%) and green light(100%) higher than in treatment with mixes of red light and green light(50:50). The effect of mineral treatment on trigonelline was shown in treatment with added Mg, Cu, Zn mineral nutrients higher than in added other mineral nutrients.
2. Selection of optimal agricultural products which are regulated metabolic diseases The relationship between diabetes mellitus and hearing function has been studied for a long time. Recent clinical studies have shown a close correlation between hearing loss and diabetes. Some studies have been reported neuronal abnormalities in diabetes can be ameliorated by treatment of neurotrophic factors such as nerve growth factor (NGF). In this study, extract of tomato (Solanumlycopersicum) increased NGF secretion in both C6cells and rat primary astrocytes and also increased neuriteoutgro wthin PC12 cells.To examine the effect of tomato extract on peripheral and midbrain auditory pathway functions,we analyzed the auditory brain stem response(ABR) and the auditory middle latency response(AMLR).The hearing thresholds of ABR and Palatencies of AMLR are increased indiabetic mice. Tomato extract suppressed the hearing threshold shiftforclicks, 4kHz and 8kHz TBs, and recovered Pa latencies. Taken together, these data indicate that auditory functional impairment from the peripheral nerve to the central nerve in diabetesis ameliorated by tomato extract, perhaps dueto NGF-regulatory activities. Therefore, we suggest that tomato may be useful as a potential treatment for diabetic auditory impairment.
The anti-obesity effects of tomato, eggplant and adlay was first investigated using 3T3-L1 preadipocytes as an in vitro system. The extract of tomato, eggplant and adlay significantly inhibited adipocyte differentiation as measured fat accumulation using Oil Red O staining. Our study also demonstrated that the extract of tomato, eggplant and adlay was more effective in the prevention of obesity. The effects of red and green tomato extracts on obesity was further investigated in high-fat diet-induced obesity in C57BL/6 mice. Body weight of mice fed HFD plus green tomato extract (GTE) was significantly decreased to the level of mice fed ND, whereas only slightly reduced in mice fed HFD plus red tomato extract (RTE). Epididymal adipose tissue and liver weights were significantly decreased in mice fed HFD plus GTE compared to those in HFD. Serum total cholesterol and low-density lipoprotein-cholesterol levels in mice fed GTE were modestly reduced, and liver total cholesterol level was strongly decreased in HFD plus GTE-fed mice compared to that in HFD-fed mice. AMPK and ACC phosphorylation in liver from HFD plus GTE-fed mice was significantly elevated, and HMGCR expression was also significantly decreased. GTE strongly decreased the expression of PPAR γ, C/EBPα, and perilipin in the adipose tissue of mice fed HFD plus GTE. Our results indicate that GTE exerted benefical effect both in vitro and in vivo assay models.

목차 Contents

• 제 출 문 ... 1
• 요 약 문 ... 2
• SUMMARY ... 5
• 제 1 장 서 론 ... 8
• 제1절 연구개발의 필요성 ... 8
• 제2절 연구개발의 목표 및 내용 ... 8
• 제2장 국내외 기술개발 현황 ... 11
• 제1절 연구개발대상 기술의 국내·외 현황 ... 11
• 제2절 국내외 연구현황 비교 및 필요 연구 분야 ... 12
• 제3장 연구개발수행 내용 및 결과 ... 14
• 제1절 연구개발수행 자원확보 및 시료전처리 ... 14
• 제2절 율무의 산성다당체 Uronic acid(Coixan) 및 Ferulic acid 분석 ... 19
• 제3절 가지(A nthocyanins) 분석법 정립 ... 25
• 제4절 토마토 과실의 alphatomatine, dehydrotomatine, Trigonelline 고함유 유전자원선발 및 Scale up기술개발 ... 27
• 제5절 대사성질환 억제효과를 가지는 농산물자원발굴 및 기전연구 ... 53
• 제4장 연구개발목표 달성도 및 대외기여도 ... 79
• 제1절 연구목표대비 달성도 ... 79
• 제2절 정량적 성과 ... 80
• 제5장 연구개발결과의 활용계획 ... 82
• 제6장 중요 변동사항 ... 83
• 제7장 참고문헌 ... 84
• <참고 1> 연차평가 지적사항 및 조치결과 ... 86
• <참고 2> 주요 연구성과 요약 ... 87
• 주요 결과 요약서 ... 89