보고서 정보
주관연구기관 |
국립원예특작과학원 National Institute of Horticultural and Herbal Science |
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 |
한국어
|
발행년월 | 2012-02 |
과제시작연도 |
2011 |
주관부처 |
농촌진흥청 Rural Development Administration(RDA) |
등록번호 |
TRKO201400000783 |
과제고유번호 |
1395022266 |
사업명 |
농업현장실용화기술개발 |
DB 구축일자 |
2014-05-10
|
DOI |
https://doi.org/10.23000/TRKO201400000783 |
초록
▼
□ 과제명 : 약용작물 고부가 소재개발 및 나노기술적용 상품성 증진 연구
▶ 연구목적
○ 약용소재에 적용 가능한 나노구조체 개발
○ 비상용 약용작물 부위 및 가공인삼으로부터 신기능성 소재 개발
○ 유용약용성분의 나노캡슐화를 통한 성분 물질의 안정성 및 전달 효율 극대화
○ 나노캡슐의 표면개질을 통한 적용성 소재 극대화
○ 약용소재에 적용 가능한 나노구조체 개발 및 바이오 재질을 이용한 약용작물 소재 상품성 향상
○ 약용작물로부터 추출/분리된 약용 소재를 이용한 피부흡수용 나노구조체 개발 및 정량
□ 과제명 : 약용작물 고부가 소재개발 및 나노기술적용 상품성 증진 연구
▶ 연구목적
○ 약용소재에 적용 가능한 나노구조체 개발
○ 비상용 약용작물 부위 및 가공인삼으로부터 신기능성 소재 개발
○ 유용약용성분의 나노캡슐화를 통한 성분 물질의 안정성 및 전달 효율 극대화
○ 나노캡슐의 표면개질을 통한 적용성 소재 극대화
○ 약용소재에 적용 가능한 나노구조체 개발 및 바이오 재질을 이용한 약용작물 소재 상품성 향상
○ 약용작물로부터 추출/분리된 약용 소재를 이용한 피부흡수용 나노구조체 개발 및 정량적 평가
▶ 주요연구내용
○ 1세부과제명 : 자원선발 및 기능성 탐색
- 기능성 후보자원으로 참당귀 선발
- TLC를 활용한 참당귀꽃의 안토시안계 물질 탐색
- 참당귀 유용성분의 HPLC 분석 조건 확립
- 참당귀 상용·비상용 부위별 유용성분 확인 및 지표성분 분석법 확립
- 압출성형처리된 참당귀의 유용성분 함량 확인 및 항산화 활성 검증
○ 2세부과제명 : 기능성 물질 소재화 연구
- 참당귀 유용성분 추출률 증대를 위한 추출용매 및 추출조건 선정
- 참당귀 유용성분 수용화를 위한 압출성형처리 최적 공정 확립
- 참당귀 압출성형물의 추출수율 및 이화학적 특성 확인
- 참당귀 다당류 추출 최적 효소처리 방법 확립
- 참당귀 활용 마스크팩 적용 가능 소재 개발
○ 3협동과제명 : 약용성분 캡슐화를 위한 나노구조체 개발
- 약용성분 캡슐화에 알맞은 나노구조체 개발
- 개발된 나노구조체와 약용성분간의 상호결합 가능성에 관한 물리 화학적 특성 확인
- 나노구조체(금속 이중층 수산화물)을 이용하여 유용성분을 캡슐화 방법 확립
- 유용성분-나노캡슐 복합체의 물리화학적 특성 평가 및 특성평가 방법 확립
- 참당귀 조추출물 함유 나노캡슐 제조방법 확립
- 캡슐화 성분의 안정성 및 항산화활성도 검증
- 나노캡슐의 응용성 극대화를 위한 코팅 및 표면 개질 방법 개발
○ 4협동과제명 : 피부흡수용 기능성 약초자원의 나노구조체 적용 기술 개발
- 인삼의 유용성분 분석 방법 확립
- 인삼 추출물을 활용한 나노에멀젼 제조 방법 확립
- 인삼 약용성분 함유 나노에멀젼의 동물 실험 모델에서의 흡수율 및 방출 거동 확인
- 나노구조체의 modification을 통한 피부 흡수율 증대 효과 검증
- 참당귀 조추출물을 활용한 나노에멀젼 제조 방법 확립
- 참당귀 조추출물 약용성분 함유 나노에멀젼의 동물실험모델에서의 흡수율 및 방출 거동 확인
Abstract
▼
To set up an functional information of a natural substance, medicinal plants were collected from 2009 to 2011. Collected plant resources were conserved in experimental field, they were made of specimens through identification and classification.
Therethrough, medicinal plants resource involved m
To set up an functional information of a natural substance, medicinal plants were collected from 2009 to 2011. Collected plant resources were conserved in experimental field, they were made of specimens through identification and classification.
Therethrough, medicinal plants resource involved material development for food and cosmetic industry was selected with Angelica gigas Nakai.
In this research, we described a optimal condition of extrusion for increasing extraction of functional components from Angelica gigas Nakai. In addition, depending on extrusion process parameters, physicochemical properties and the yield of soluble extract were analyzed. As the result of statistical analysis of response surface methodology(RSM), the maximum extract contents of decursin and decursinol angelate was obtained when screw speed was 61.33rpm, feed rate 35.67kg/h, water feed rate 4.8kg/h. The water-soluble components of Angelica gigas Nakai extrudate increased by 21.75% compared with the control, these was significantly different(p>0.05). Among the functional components of Angelica gigas Nakai, total sugar, total polyphenol, total flavonoid, decursin and decursinol angelate was increased by 6.64%, 40%, 42.5%, and 16.64%, respectively, compared with the control. The antioxidant activity of Angelica gigas Nakai extrudate of the optimum condition was measured 10.29∼14.59% compared with control. We was performed to measure influence of the solubilization of the polysaccharide, to establish extraction methods of the polysaccharide at different enzyme treatment and to evaluate the physicochemical properties for maximizing extract rate of functional components from Angelica gigas Nakai. The total sugar content of VAFT(Viscozyme, Alcalase/flavourzyme, Termamyl) treated with enzyme, 76.80% was higher than that of T(Termamyl) 53.50% and AFT(Alcalase/flavourzyme, Termamyl) 51.61%. In addition, neutral sugar and uronic acid were the highest in VAFT. The molecular weight of VAFT was decreased from 491,000Da to 13,000Da in low molecular weight compared to those of control. Non-starch polysaccharide(NSP) contents of Angelica gigas Nakai. extrudate was increased than those of control. These results suggest that water soluble polysaccharides(WSP) obtained using extrusion and enzymatic treatment may be an effective method to produce NSP and neutral sugar from Angelica gigas Nakai and be used as a functional food and cosmetic ingredients.
We mainly focused on the application of nanotechnology to maximize the applicability of medicinal crops. In order to achieve the final goal, "The stabilization and property control of medicinal crops extract utilizing nanostructured materials", we have systematically carried out researches for 3 years. In the first year, we have investigated the nanostructured materials which can be applied to the effective elements in medicinal crops, and synthesized nanostructres through various synthetic routes, and studied the physico-chemical interaction between nanostructures and effective elements in medicinal crops. As a result, we determined layered double hydroxides as a nanomaterials which can stabilize the effective elements, maximize the beneficial property and have biocompatibility. In the second year, we have studied the nanoencapsulation of ferulic acid, an abundant polyphenol found in natural substances. The ferulic acid was chosen as a model compound in order to study the possibility of nanostructures stabilizing effective elements in medicinal crops. We have studied various nano-encapsulation methods, physico-chemical stabilization of encapsulated molecules, and the ferulic acid release properties with respect to encapsulation method to finally deduce the optimal nanoencapsulation method. In the third year, utilizing the results of the previous year, we have tried to stabilize the extract of Angelica gigas Nakai, one of medicinal crops, and studied the anti-oxidant effect of encapsulated extract. It was verified that the extract encapsulated by nanostrctures can be stabilized from the external stimulation such as ultraviolet irradiation, and the anti-oxidant effect can be preserved by encapsulation. As a result of this project, we could determined a encapsulation method of medicinal crops extract utilizing nanostructures. And it was also proven that the encapsulation with nanostructures can enhance the intrinsic beneficial properties of medicinal crops extract. These results, in the future, are thought to be applied to the extract from various kinds of medicinal crops.
Synthesis and characterization of nanostructures are world-wide interesting research field.
And we can use nanotechnology for enhancing stability and absorption of active compound extracted from medicinal crop. Based on this background, the aim of this study was to prepare nanoemulsion system of medicinal crop for enhancing topical delivery of active compound extracted from medicinal crop. For prepare nanoemulsion system, nanoemulsion formulations were selected at different component ratios and size and characterized by solubility, particle size and so on. In vitro and in vivo study were conducted to determine the amount of active compound retained in the skin. This study was conducted using processed ginseng and Angelica gigas Nakai, Rg3 and decursin were selected as markers for quantification. Nanoemulsions of processed ginseng and root extract of Angelica gigas Nakai were prepared using pseudo-ternary phase diagrams and characterized by solubility, particle size, and morphology. After nanoemulsion formulations were selected, in vitro and in vivo skin deposition study was conducted. Permeation ability of nanoemulsion through skins and into skins was higher compared to the control group. The results obtained contribute to the activation of studies of medicinal crop using nanotechnology and the development of functional foods and cosmetics using medicinal crop.
목차 Contents
- 제출문 ... 1
- 요 약 문 ... 2
- SUMMARY ... 6
- 제1장 서 론 ... 8
- 제2장 국내외 기술개발 현황 ... 9
- 제1절 국외 기술개발 현황 ... 9
- 제2절 국내 기술개발 현황 ... 10
- 제3절 국내·외 연구현황 ... 11
- 제3장 연구개발수행 내용 및 결과 ... 12
- 제1절 자원선발 및 기능성 탐색(제1세부연구과제) ... 12
- 제2절 기능성 물질 소재화 연구(제2세부연구과제) ... 22
- 제3절 약용성분 캡슐화를 위한 나노구조체 개발(제3협동연구과제) ... 36
- 제4절 피부흡수용 기능성 약초자원의 나노구조체 적용기술 개발 (제4협동연구과제) ... 53
- 제4장 연구개발목표 달성도 및 대외기여도 ... 63
- 제1절 목표대비 달성도 ... 63
- 제2절 정량적 성과 ... 64
- 제5장 연구개발결과의 활용계획 ... 67
- 제6장 중요 변동사항 ... 68
- 1. 세부과제 통합 및 명칭 변경(공문 약용작물과-213, 2010.2.23.) ... 68
- 2. 주관과제 및 세부과제 책임자 변경 ... 68
- 3. 제2세부과제 연구내용 변경(공문 약용작물과-656, 2010.6.8.) ... 68
- 제7장 참고문헌 ... 69
- <참고 1> 연차평가 지적사항 및 조치결과 ... 74
- <참고 2> 주요연구 성과 ... 75
- 주요 결과 요약서 ... 76
※ AI-Helper는 부적절한 답변을 할 수 있습니다.