Abstract ▼

Dental Bone substitutes are used as scaffold due to their osteo-inductive property. However, they are hard to maintain their morphology and degrade in vivo. Therefore, for the effective bone tissue regeneration, there is need to develop the fountional scaffold system, such as loading of TGF-β, BMP,

Dental Bone substitutes are used as scaffold due to their osteo-inductive property. However, they are hard to maintain their morphology and degrade in vivo. Therefore, for the effective bone tissue regeneration, there is need to develop the fountional scaffold system, such as loading of TGF-β, BMP, and simvastatin, and sustained release system. For the dental bone tissue regeneration, photo-curable hydrogel is fabricated using hyaluronic acid which is biocompatible and non-toxic. Hyaluronic acid grafted aminoethyl methacrylate are composed photo-curable hydrogel to loaded simvastatin. For fabrication of photo-curable hyaluronic acid (HA) based hydrogel, aminoethyl methacrylate (AEMA) is cross-linked to the carboxyl group on the HA using EDC/NHS. To determine the feasibility of AEMA treated HA hydrogel as a simvastatin delivery carrier, the release kinetics of simvastatin was examined using an UV spectrophotometer. When 0.5 and 1 mg of simvastatin was loaded, the rapid release at early time was detected with following sustained release from AEMA treated HA hydrogel. And then, the dental pulp stem cell is isolated at the extracted tooth to characterize osteogenic differentiation with simvastatin. The isolated dental pulp stem cell was characterized from simvastain and specific gene expressions (MMP, Cathepsin K) was evaluated. The advantages of needle injection are simple treatment and short operating time. And non-invasive method could lower recovery time and complications such as infection, hematoma, and chronic pain. And faster return to work field of patient could reduce the loss of manpower.

목차 Contents

• 표지 ... 1
• 제출문 ... 3
• I. 총괄현황 ... 4
• 3. 보고서 요약서 ... 5
• 4. 요약문 (한글) ... 6
• 5. 요약문 (영문) ... 7
• 6. 연구 성과 실적 및 향후 계획 ... 8
• 7. 참여연구원 현황표 ... 12
• Ⅱ. 총괄연구과제 연구결과 ... 13
• 1. 연구개발과제의 배경 및 필요성 ... 14
• 2. 국내외 기술개발 현황 ... 15
• 3. 연구개발과제의 추진체계 ... 22
• 4. 연구개발수행 내용 및 결과 ... 23
• 5. 목표달성도 및 관련분야 기여도 ... 49
• 6. 향후 연구성과 추진 계획 ... 50
• 7. 연구개발결과의 파급효과 ... 50
• 8. 연구개발결과의 활용계획 ... 52
• 9. 연구개발과정에서 수집한 해외과학기술정보 ... 52
• 10. 참고문헌 ... 52
• Ⅲ. 첨부서류 ... 54
• 끝페이지 ... 54