보고서 정보
주관연구기관 |
국립축산과학원 National Institute of Animal Science |
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 |
한국어
|
발행년월 | 2014-02 |
과제시작연도 |
2011 |
주관부처 |
농촌진흥청 Rural Development Administration(RDA) |
등록번호 |
TRKO201400011384 |
과제고유번호 |
1395022458 |
사업명 |
축산생명환경시험연구 |
DB 구축일자 |
2014-06-28
|
DOI |
https://doi.org/10.23000/TRKO201400011384 |
초록
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Ⅳ. 연구개발결과
○ 1세부과제명 : 섬유소 강화 악취저감 사료 개발
- 비트펄프 또는 IRG 건초 분말을 기초사료에 각각 5% 첨가하여 급여하였을 때 일당증체량 등 사양성적은 차이가 없었다. 악취물질 농도는 대조구에 비해 비트펄프구와 IRG구에서 높았다.
- 사료에 서양고추냉이 분말 0.03%, 팽이버섯폐배지 0.5% 및 서양고추냉이 분말 0.03%와 팽이버섯폐배지 0.5%의 혼합제를 사료에 첨가하였을 때 슬러리의 인돌류 농도는 고추냉이와 팽이버섯폐배지의 혼합구에서 가장 낮았다.
- 돈사악취 저감사료 실증 시
Ⅳ. 연구개발결과
○ 1세부과제명 : 섬유소 강화 악취저감 사료 개발
- 비트펄프 또는 IRG 건초 분말을 기초사료에 각각 5% 첨가하여 급여하였을 때 일당증체량 등 사양성적은 차이가 없었다. 악취물질 농도는 대조구에 비해 비트펄프구와 IRG구에서 높았다.
- 사료에 서양고추냉이 분말 0.03%, 팽이버섯폐배지 0.5% 및 서양고추냉이 분말 0.03%와 팽이버섯폐배지 0.5%의 혼합제를 사료에 첨가하였을 때 슬러리의 인돌류 농도는 고추냉이와 팽이버섯폐배지의 혼합구에서 가장 낮았다.
- 돈사악취 저감사료 실증 시험에서 기초사료에 IRG 펠렛을 0.5% 혼합하였을 때에 일당증체량, 사료섭취량 및 사료효율은 변화가 없었으며(p> 0.05), 돈사 슬러리에서 SCFA, BCFA, 페놀류 및 인돌류 농도는 감소하였다.
○ 2세부과제명 : 돈사 피트내 슬러리의 악취 저감용 환경 개선물질 선발
- 기초사료 급여 슬러리에 버섯배지를 1.0% 첨가하였을 때 인돌류 증가율이 대조구보다 49% 감소하였고, 단쇄지방산의 농도는 감소되지 않았다.
- 슬러리를 6주간 저장하였을 때 저장기간이 경과함에 따라 페놀류와 단쇄지방산 농도는 감소되었으나, 인돌류와 이성체 지방산 농도는 증가되었다
- IRG 0.5% 혼합사료 급여 슬러리와 IRG 0.5% 혼합사료 급여 슬러리에 팽이버섯폐배지 1%첨가구 모두 슬러리의 저장기간이 경과함에 따라 분뇨의 페놀류, 인돌류, 단쇄지방산과 이성체지방산의 농도는 증가되었다. 슬러리 저장기간이 같을 때 무처리구에비하여 팽이버섯폐배지구에서 페놀류, 단쇄지방산과 암모니아성 질소 농도가 더 높았으나, 인돌류는 팽이버섯폐배지구에서 더 낮았다.
○ 3세부과제명 : 악취저감제제 효능 평가기술 개발
- 돈분 슬러리를 배양온도 및 기간별 성상변화(미생물 등)를 분석한 결과 20도, 37도의 시료에서 종합악취 및 미생물의 군집변화가 유의적으로 나타났다.
- Electronic Nose를 통한 종합악취성분 패턴에 있어서 1주차부터 시료간 차이를 보였으며 3주차 이후 20도, 37도 처리구의 악취패턴 차이가 명확히 나타났다.
- 간이배양장치내의 전용페트리접시에 돼지 분뇨를 위치시킨 후 미생물배양액을 분사하였을 때 P aenibacillus속 등 미생물배양액이 암모니아와 황화수소의 감소효과를 나타냈다.
○ 4세부과제명 : 액비화 과정 중 인 이용 우수미생물 선발 및 활용
- 돈분슬러리액에는 Acinetobacter towneri 등이, 액비저장액에는 Bacillus licheniformis 등 Bacillus 계열의 미생물들이 스크리닝되었다.
- 고인산 조건에서는 Uncultured bacterium clone가 주로 동정되었으며, 인이용 미생물로 알려진 Acinetobacter보다 인이용성에 있어서 우수했다.
- 후보균주의 생존성을 보기위해 X-인산배지에 액비의 첨가수준을 15, 30%로 하여 4주간의 장기간 배양한 결과, 혐기조건에서우수한 생존능을 보였다.
- 후보균주를 이용하여 배지내 인이용능을 조사한 결과 전 배양기간동안 P aenibacillus sp.가 이용효율면에서 우수한 결과를 보였다.
○ 5세부과제명 : 바이오가스 소화액을 이용한 사료작물 시용효과 구명
- 수수×수단그라스의 ha당 생산량은 소화액100%구의 생산량이 15,574kg으로 화학비료구의 생산량 16,572kg에 비해 94%의 생산량을 나타내 가장 높았고, 무비구는 81.2%의 생산량을 보였다. 호밀은 ha당 생산량이 소화액150%구의 생산량이 7,993kg으로 화학비료구의 생산량 7,647kg에 비해 104.5%의 생산량을 나타내어 가장 높았으며 무비구의 생산량은 50.7%이었다.
- 바이오가스소화액 및 SCB액비의 이용이 여러 작물로 확대되고 있어, 농가의 생산성을 향상을 위해서 작물별로 소화액의 시용기준 및 시용방법을 명확히 제시하는 것이 필요하다
Abstract
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This study was performed to reduce odor from pig pen through fermentation control. in vitro and in vivo fermentation capability analysis was carried out for beet pulp, horseradish powder, italian ryegrass powder to select the feedstuff for pig diet to reduce odor emis
This study was performed to reduce odor from pig pen through fermentation control. in vitro and in vivo fermentation capability analysis was carried out for beet pulp, horseradish powder, italian ryegrass powder to select the feedstuff for pig diet to reduce odor emission in pig slurry. The index about fermentation capability was chosen according to the experiment condition. We investigated the levels of concentration of odorous compounds including phenols, indoles, volatile fatty acid in slurry. It was found that horseradish powder decreased concentration of odorous compounds in slurry. And then, the amount of horseradish powder in basal diet needs to be identified. There was no difference between the effects of beet pulp and italian ryegrass powder on levels of VFA, phenols and indoles in pig slurry. Mixed ratio of horseradish powder with pig diet was approximately 0.03%. Average daily gain, feed intake and feed efficiency was similar between italian rye grass power treatment and control when italian rye grass power was mixed 0.5% in the control diet. The concentration of VFA, phenols and indoles in pig slurry was reduced compared with that of control. Data from our current study suggest that the ideal level of beet pulp and italian ryegrass powder to be mixed with diet to manufacture odor reducing diet was approximately 0.5%.
This study was performed 1) to select fermentation acceleration agent, 2) to investigate the concentration of odorous compounds in response to slurry storage time as well as fermentation acceleration materials, and 3) to evaluate theAs storage period of manure slurry both for control and 1% spent mushroom treatment group increased, levels of phenols, indoles, short chain fatty acids and isomeric fatty acids were increased (P<0.05). Effect of supplementary diets and fermentation accleration agent on reducing the odorous compounds in pig slurry. Concentration of short chain fatty acids was not affected but indoles were reduced by 49% in 1% spend mushroom supplement group than in control group. The concentration of indoles in slurry was reduced 49% compared with that of control. Concentration of short chain fatty acids was not affected but indoles were reduced by 49% in 1% spend mushroom supplement group than in control group. The concentration of indoles was decreased when 1% spent mushroom was added in pig slurry. Fermentation acceleration agent treatment decreased indole level by 29% when compared to control group. Levels of phenols and short chain fatty acids were decreased (P<0.05) but indoles and fatty acids were increased (P<0.05) when slurry was stored for 6 wk. IRG tended to decrease the levels of phenoles, short chain fatty acids and isomeric fatty acids and ammonium nitrogen but increase the level of indoles. As storage period of manure slurry both for control and 1% spent mushroom treatment group increased, levels of phenols, indoles, short chain fatty acids and isomeric fatty acids were increased (P<0.05). Levels of phenoles, shortchain fatty acid, isomeric fatty acid and ammonium nitrogen were increased (P<0.05) but level of indole and pH was lower in spent mushroom treatment group than in control group when the storage periods were the same. As stroage period of manure slurry both for 0.5% IRG and 0.5% IRG + spent mushroom groups increased, the levels of phenols, indoles, short chain fatty acid and isomeric fatty acids were increased (P<0.05). Levels of phenoles, short chain fatty acids and ammonium nitrogen were increased (P<0.05) but level of indole was lower (P<0.05) in spent mushroom group than in control group when the storage periods were the same. Taken together, our data showed that the longer the storage time, the greater the levels of phenols, indoles, short chain fatty acids and isomeric fatty acids in slurry. Furthermore, spent mushroom and IRG had beneficial effect on reducing the odorous compounds in pig slurry.
This study was designed to develop the assessment techniques for the odor factors of swine manure in various analytic aspects. We investigated the effects of the variation of temperatures and times intervals on the odor patterns of swine manure using the development of bacterial community analysis. Fresh swine manure was collected by scrapers in the roofed manure storage place of National Institute of Animal Science (NIAS), Suwon, South Korea. The Solid manure was added with distilled water to increase the moisture content to 80% and there by immediately subjected to filtration through 1 x 1 sieves to homogenize the particle size. 50ml of swine manure was dispensed aerobically into serum bottles and were incubated in – 20, 4, 20 and 37°C incubator for 4 weeks. The bacterial samples were analyzed using MALDI-TOF technique and pyrosequencing of 16S rRNA gene. The odor pattern of swine manure was analyzed with electronic nose system during incubation time. To evaluate the effects of commercial deodorants on odor mitigation of swine manure, the chamber was designed to assess relative de-odor values. These results indicated that the richness of bacterial community in sample was reduced by temperature and incubation periods. Firmicutes was dominant phylum in all of samples (ranging from 89.3% to 98.8% of total reads) and Actinobacteria was second dominant groups in most of samples (0.6% to 7.9%). The compositions of community changed from 1week at 37°C incubation condition, this showed us temperature is more significant factors to influence on microbiota in swine manure. The odor pattern of swine manure was also similar to the results of microbial communities. Genera of Clostridium, Turicibacter, Streptococcus, and Lactobacillus within Firmicutes and Corynebacterium within Actinobacteria were dominant in original manure and most of incubated samples. The proportions of dominant genera were changed in 4 weeks incubated sample at 20°C and all incubated samples at 37°C. Bacterial communities in swine manure were significantly changed in 20°C and 37°C incubation samples. This study revealed that the microbial community changes rapidly with the variation of time and temperature during decomposition of manure characterized by the predominance of Firmicute and Actinobacteria. Our results served as a model for development of the comparative assessment for odor emission in swine farm.
Microorganisms producing in Liquid compost process for pig slurry and liquid fertilizer sampling and the growth stimulation and phosphorus ability were investigated. Storage liquid compost and pig slurry using microorganisms number, type and microbial population investigated by agar plate count and the MALDI-TOF technique has revealed Acinetobacter towneri such as pig slurry, Storage liquid compost with department of Bacillus class such as Bacillus licheniformis have been screened. Using the X-phosphate MOPS medium in isolated and identified strains of phosphorus ability, with the addition of 2mM K2HPO4 in addition, it was isolated and identified in high phosphoric acid conditions. As result of the culture liquid fertilizer microbial and slurry liquid in the high phosphoric acid and low phosphoric conditions, slurry and liquid fertilizer contains phosphorus available to determine because there are many microorganisms and microbial growth rate, and in only two colonies are formed depending on the capacity considered. After the pure culture by selecting only the microbial communities of the high phosphoric acid medium, we conducted nucleotide sequence analysis but, Acinetobacter regardless of the concentration of phosphoric acid was primarily investigated. Uncultured bacterium clone, which is mainly sympathy high phosphoric acid conditions, and was excellent in the use of such Acinetobacter than it is known as a microbial phosphorus ability. Results of investigate the proliferation capacity of a candidate strain, and compared the proliferative capacity of subculture and after freeze / thawing after cultivation of the same strain in an increase of 3 times up to 60 hours after culture, showed a difference in food. As a result of the 15, 30% addition level of liquid fertilizer to the X-phosphoric acid medium, and long term culture of 4 weeks to see the viability of the candidate strain, and showed excellent survival ability under anaerobic conditions. phosphorus ability. Using the candidate strain in medium phosphorus availability in culture medium, during the pre-incubation period Paenibacillus sp. AEY-1 were used showed excellent results in terms of efficiency.
The objective of this study was to suggest use standard of digestion waste fluid, investigate the productivity of crops and substitution effect of chemical fertillizer on biogas digestion waste fluid. The fertillization effect of digestion waste fluid for sorghum sudangrass hybrid and rye was as in the following. In production of sorghum sudangrass hybrid by test port, plot of digestion waste fluid 100% was largest as 94% of chemical fertillizer plot. on the other hand, In production of rye by test port for fertillization effect of digestion waste fluid, the plot of digestion waste fluid 150% was largest as 104.5% of chemical fertillizer plot. In latly, biogas digestion waste fluid and SCB liquid fertillizer expand the use on several plant and fruiter. how to use and standard manuring level of biogas digestion waste fluid by crops should be suggested for productivity improvement of farm in korea.
목차 Contents
- 표지 ... 1
- 제 출 문 ... 2
- 요 약 문 ... 3
- SUMMARY ... 6
- 목차 ... 9
- 제 1 장 서 론 ... 10
- 제 2 장 국내외 기술개발 현황 ... 14
- 제 1절 국·내외 관련분야에 대한 기술개발 현황 ... 14
- 제 2절 연구결과가 국·내외 기술개발현황에서 차지하는 위치 ... 17
- 제 3 장 연구개발 수행 내용 및 결과 ... 18
- 제 1 절 섬유소 강화 악취저감사료 개발 ... 18
- 제 2 절 돈사 피트내 슬러리의 악취 저감용 환경 개선물질 선발 ... 34
- 제 3 절 악취저감제제 효능 평가기술 개발 ... 47
- 제 4 절 액비화 과정 중 인 이용 우수미생물 선발 및 활용 ... 57
- 제 5 절 바이오가스 소화액을 이용한 사료작물 시용효과 구명 ... 63
- 제 4 장 연구개발 목표 달성도 및 대외 기여도 ... 67
- 제 1 절 목표대비 달성도 ... 67
- 제 2 절 정량적 성과 ... 68
- 제 3 절 관련분야의 기술발전 기여도 ... 72
- 제 5 장 연구개발결과의 활용계획 ... 73
- 제 6 장 연구개발과정에서 수집한 해외과학기술정보 ... 73
- 제 7 장 기타 중요 변동사항 ... 73
- 제 8 장 국가과학기술종합정보시스템에 등록한 연구장비 현황 ... 73
- 제 9 장 참고문헌 ... 74
- 끝페이지 ... 82
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