한국보건산업진흥원 Korea Health Industry Development Institute
등록번호
TRKO201400016216
과제고유번호
1460001147
사업명
보건의료기술연구개발(보건의료기술인프라개발)
DB 구축일자
2014-11-10
키워드
신변종 바이러스.인플루엔자.급성호흡기증후군.Emerging and reemerging virus.influenza.acute respiratory syndrome.HIV.HCV.WNV.
초록▼
연구결과 및 성과 ○ 국내유행 신변종 바이러스 분자역학 및 유전자 Database 구축 ○ 바이러스 매개 야생조류의 생태분석 및 질병매개 감시대상 조류 국가감시체계안 구축 ○ 인간 조류인플루엔자 바이러스 제어기술 및 연구기반 구축 ○ 조류인플루엔자 신속진단을 위한 기반기술개발 ○ 사스 코로나바이러스 세포 독성 메카니즘 연구 ○ 사스바이러스 감별진단을 위한 Human metapneumovirus 조기진단 및 역학 ○ 사스 대비 국제협력체계 구축 ○ HCV 증식저해기전 연구를 위한 in vit
연구결과 및 성과 ○ 국내유행 신변종 바이러스 분자역학 및 유전자 Database 구축 ○ 바이러스 매개 야생조류의 생태분석 및 질병매개 감시대상 조류 국가감시체계안 구축 ○ 인간 조류인플루엔자 바이러스 제어기술 및 연구기반 구축 ○ 조류인플루엔자 신속진단을 위한 기반기술개발 ○ 사스 코로나바이러스 세포 독성 메카니즘 연구 ○ 사스바이러스 감별진단을 위한 Human metapneumovirus 조기진단 및 역학 ○ 사스 대비 국제협력체계 구축 ○ HCV 증식저해기전 연구를 위한 in vitro system 구축: HCV replicon ○ HCV 유전자형에 따른 치료효과 예측기반연구: 인터페론 치료반응 영향 인자 분석체계 ○ HCV 증식제어 및 초기면역반응 연구시스템 구축 ○ PTD를 이용한 재조합 HIV 단백질을 HIV 체액성 면역반츰 측정 표준화 연구 ○ HlV 세포성 면역반응 측정 표준화 연구 ○ HIV 진화압력요인분석 ; 치료제 내성 및 면역회피기전 ○ 신종 한탄바이러스 분자혈청학적 연구와 제어기술기반연구 ○ 웨스트나일 바이러스 연구기반 구축
Abstract▼
Results Part I Foundation Study and Analysis of Factors on Emerging and Reemerging Viruses We secured gene sequence data of viruses isolated in Korea by literature search and established viral database with application of NCBI registered gene sequences. The purpose of this gene sequence databa
Results Part I Foundation Study and Analysis of Factors on Emerging and Reemerging Viruses We secured gene sequence data of viruses isolated in Korea by literature search and established viral database with application of NCBI registered gene sequences. The purpose of this gene sequence database is secondary DB establishment to utilize registered genes with useful epidemiological information, not sequence-accumulating primary DB. This DB is supposed to expand as a KCDC's project (Early detection of emerging and reemerging pathogen: Virus DB) from 2006. This DB makes it possible to study differnces, regional characteristics, trend by year of pathogens. Furthermore, through study on ecological characteristics of vector birds. we had selected kinds, methods and national surveillance plan on subject birds Part II Control and Prevention of Acute Respiratory Viruses With acute respiratory viruses like Influenza, AI, metapneumovirus, and SARS virus, our aim is to develop technology to secure lab scientists' security for emerging pathogen occurrence, and to enhance to facilitate diagnosis; we have also aimed to develop techniques to make artificial gene synthesis, which makes it as foundation technology in this field. For influenza virus, we made antibody library to virus induced proteins and proved that these antibodies are bonded specifically. For NP protein of influenza, we proved efficiency as diagnostic antigen by optimal new NP synthesis and large manifestation from yeast. For AI, we identified possibility of human infection through antiserum and gene analysis on sera of wild birds and domestic poultry For SARS virus study, antigen of corona virus manifest in cells of mammals and we can get cell toxic results through each isolate antigen. We also developed a diagnosis using Multiplex RT-PCR with metapneumovirus which makes SARS diagnosis confusing, and made a basis for distinct diagnosis for lower respiratory tract infection. Part III Study on Infection Mechanism and Control and Prevention for Hepatitis C We have analytical system on influencing factors to treatment reaction of interferon: viral genes, gene sequence of specific part like HVR in E2 antigen, and ISDR in NS5Q, and HCV RNA concentration before treatment. Especially, using recently developed replicon system, analysis on viral pressure mechanism of interferon revealed that PKR induced by interferon plays very important role. Interestingly, when manifestation of PKR is pressed, viral replication is increased by 30%, compared with normal cells. This result implies that PKR is not only an important medium for viral treatment of interferon externally medicated, but also an important factor to host cell's resistance. We observed that nother host protein induced by interferon, ISG15 reduced the stability of NS5A in HCV and proved that interferon signal transmission is very important in early antivirus reaction of virus infection. Part IV HIV/AIDS Immune Response and Evolvement Factor Analysis To overcome AIDS, effective AIDS vaccine is absolutely necessary. To develop effective vaccine and test efficiency, measurement of cellular, humoral immune response should be standardized and quantified. Along with that, HIV variation according' HIV immune selection pressure and drug resistance pressure in Korean HIV infected persons need to be prospectively studied. This study produced recombinated PTD-Gag protein using PTD(Protein transduction domain)(PTD), and make it react with PBMC isolated from patients by IFN-γ ELISPOT meaured immune response. This will be basic data to develop HIV vaccine for prevention and treatment and will be used to confirm upcoming AIDS vaccine's efficacy. We also produced MuIV-HIV pseudotyped virus to measure humoral immune response with Korean HIV-1 infected persons, using HIV-1 virus envelope gene; we identified the possibility of developing measurement method of common neutral antibody activity founded in 70% of the Korean HIV-1 infected and optimal antigen HIV/AIDS vaccine. We also identified immunologiccal characteristc of Korean HIV infected persons and AIDS patients and secured basic data for proper combination of immunological treatments and antiretroviral treatments. Part V Establishment of Research Foundation on Emerging and Reemerging Vector-borne Viruses Study on obtaining systematical gene information, pathogenecity, and patient detection method on emerging Hantavirus is urgent for national security. To establish patient detection method on emerging Hantavirus, we developed diagnostic method to distinguish serum types. We also established recombination and redistribution technology for genesis mechanism of emerging and artificial hantavirus Furthermore, from viral RNA of WNV 8956, West Nile virus reference strain, we synthesized 4 cDNA amplicon and successfully inserted them into pBeloBAC plasmid as 1 full-length eDNA, which means we synthesized 1 full-length cDNA molecular clone and identified self-reproduction.
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