초록 ▼

○ 종양에서 추출한 엑소좀을 세포표지자인 [^131/124I]HIB를 이용해 성공적으로 표지하고 분리하였음.
○ 방광 종양 특이적 펩타이드 (BP) 를 Ga-68로 표지한 후 microPET 영상연구를 수행함.
○ [¹⁸F]TFB의 성공적으로 합성하고 [¹⁸F]TFB가 serum에서 4시간까지 안정함을 확인함.
○ 신생혈관형성 영상을 위한 ⁶⁸Ga/⁶⁴Cu로 표지된 RGD 유도체의 합성하고 신생 혈관형성에 높은 섭취를 보

○ 종양에서 추출한 엑소좀을 세포표지자인 [^131/124I]HIB를 이용해 성공적으로 표지하고 분리하였음.
○ 방광 종양 특이적 펩타이드 (BP) 를 Ga-68로 표지한 후 microPET 영상연구를 수행함.
○ [¹⁸F]TFB의 성공적으로 합성하고 [¹⁸F]TFB가 serum에서 4시간까지 안정함을 확인함.
○ 신생혈관형성 영상을 위한 ⁶⁸Ga/⁶⁴Cu로 표지된 RGD 유도체의 합성하고 신생 혈관형성에 높은 섭취를 보임을 확인함.
○ [^131/124I]HIB를 이용하여 지방유래줄기세포를 심근 주입후 2주간 성공적으로 영상화함.
○ [¹²⁴]ApoPep 기반의 세포사멸 영상연구를 통한 세포치료효과를 조기에 검증할 수 있음을 확인함.
○ 소동물에서 ⁶⁴Cu-RGD를 이용한 종양 진단 연구를 성공적으로 진행함.

Abstract ▼

○ Imaging study on exosome using [^131/124I]HIB
- [^131/124I]HIB was synthesized in high yield using solvent extraction method.
- Exosome was successfully collected from MCF-7 tumor cells
- Exosome collected from tumor cells was successfully radiolabeled with [^131/1

○ Imaging study on exosome using [^131/124I]HIB
- [^131/124I]HIB was synthesized in high yield using solvent extraction method.
- Exosome was successfully collected from MCF-7 tumor cells
- Exosome collected from tumor cells was successfully radiolabeled with [^131/124I]HIB.
- MicroPET imaging study revealed that exosome was transported to mainly liver and spleen.
○ Development of tumor imaging agent using [⁶⁸Ga]Y-BP
- Efficient elution method of Ga-68 was established.
- Bladder-specific peptide (BP) was conjugated with NOTA-NCS to give NOTA-BP.
- NOTA-BP was radiolabeled with Ga-68 and successfully purified using HPLC.
- ⁶⁸Ga-NOTA-BP showed high stability in serum and found to be cleared out through kidney based on microPET studies.
○ Synthesis and characterization studies of [¹⁸F]TFB
- [¹⁸F]TFB was successfully radiolabeled with F-18 and purified using simple cartridge.
- [¹⁸F]TFB showed high stability in serum up to 4h.
○ Synthesis of ⁶⁸Ga/⁶⁴Cu-labeled RGD derivatives for angiogenesis imaging
- RGD was conjugated with NOTA-NCS for ⁶⁸Ga radiolabeling.
- NOTA-RGD showed as high affinity for αvβ₃ as parent RGD itself.
- New TE2A derivatives were developed for efficient radiolabeling of RGD with Cu-64.
- Radiolabeled ⁶⁴Cu-TE2A-NCS-c(RGDyK) showed high tumor uptake in biodistribution studies.
○ Cell trafficking study using [^131/124I]HIB
- Adipose-derived stem cell was radiolabeled in high yield using [^131/124I]HIB
- Radiolabeled stem cell was injected into myocardium and followed up to 2 weeks using PET and SPECT
- The radiolabeled stem cell was successfully differentiated into various cells without any significant cell damage
- [^131/124I]HIB-labeled liposome showed high tumor uptake with very low background signals
○ Apoptosis imaging study using [¹²⁴I/⁶⁸Ga]ApoPep
- ApoPep was conjugated with NOTA and successfully radiolabeled with Ga-68
- Chemotherapeutic effect was evaluated early using non-invasive nuclear imaging studies of [¹²⁴I]ApoPep
○ Angiogenesis imaging study using ⁶⁴Cu-radiolabeled RGD
- New bifunctional chelator was synthesized in order to radiolabel with Cu-64 efficiently
- RGD peptide was conjugated with new BFC and radiolabeled with Cu-64 in high yield
- New blood vessels was successfully imaged in animal tumor models using [⁶⁴Cu]RGD

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 보고서 요약서 ... 3
• 요 약 문 ... 5
• SUMMARY ... 8
• CONTENTS ... 10
• 목 차 ... 10
• 제1장 연구개발과제의 개요 ... 11
• 1절. 연구개발의 목적 ... 11
• 2절. 연구개발의 필요성 ... 11
• 제2장 국내외 기술개발 현황 ... 13
• 1절. 국내 기술개발현황 ... 13
• 2절. 국외 기술개발현황 ... 13
• 3절. 현기술상태의 취약성 ... 14
• 제3장 연구개발수행 내용 및 결과 ... 15
• 1절. 세포 표지용 방사성 추적자 개발 및 활용 연구 ... 15
• 2절. 치료효과 판정 방사성 프로브 개발 및 활용 연구 ... 26
• 3절. 종양진단용 방사성 프로브 개발 및 활용 연구 ... 33
• 제4장 목표달성도 및 관련분야에의 기여도 ... 52
• 1절. 목표달성도 ... 52
• 2절. 관련분야에의 기여도 ... 53
• 제5장 연구개발결과의 활용계획 ... 55
• 1절. 추가연구의 필요성 ... 55
• 2절. 연구개발결과의 활용계획 ... 55
• 제6장 연구개발과정에서 수집한 해외과학기술정보 ... 57
• 끝페이지 ... 59