초록

○ 연구결과
- 잣버섯 추출물 및 생리활성성분의 대식세포 및 사이토카인 활성화 작용 기전을 규명하였음.
- 잣버섯 자실체 추출물의 제형 연구를 통한 시제품 생산 완료하고 기능성 식품으로서의 개발을 위한 안전성 확보를 위해 잣버섯 자실체 추출물의 설치류 반복독성시험을 실시하여 유효용량의 10배량까지도 안전함을 확인하였음.
- 우량 액체종균을 선발하고 이를 접종하여 자실체를 대량재배할 수 있는 기술을 확보하고 이들의 생산을 종균별로 비교하므로서 최적의 대량생산 시스템을 확립하였음.

Abstract ▼

Mushrooms are known to have diverse beneficial biological activities such as anti-cancer, immunomodulation, anti-bacteria and anti-virus. However, the molecular mechanisms underlying biological effects of many mushroom-derived materials have not yet been clearly elucidated. In this study, the immuno

Mushrooms are known to have diverse beneficial biological activities such as anti-cancer, immunomodulation, anti-bacteria and anti-virus. However, the molecular mechanisms underlying biological effects of many mushroom-derived materials have not yet been clearly elucidated. In this study, the immunomodulating activity and its molecular mechanisms of a water-soluble extract from Lentinus lepideus mycelium, named PG101, were investigated with an aim at developing it as an immunomodulating and hematopoietic agent, by performing in vitro and in vivo experimentals.
First, the effects of PG101 on human cells were investigated by analyzing the production of various cytokines using human peripheral blood mononuclear cells (PBMC). Treatment with PG101 increased the levels of TNF-, IL-1, IL-10, IL-12, GM-CSF and IL-18 in a dose- and time-dependent manner. The cytokines were expressed in a specific order respectively as following [$TNF-{\rightarrow}IL-1{\rightarrow}IL-12,\;IL-10{\rightarrow}GM-CSF,\;IL-18$]. When the target cell types of PG101 was examined, CD11b-positive cells isolated from PBMCs and a human promonocytic cell line (U937) were responsive to PG101. Data from a transient transfection assay using reporter plasmids, a gel retardation assay and the experiment involving a specific NF-B inhibitor, PDTC (pyrrolidinedithiocarbamate), indicated that NF-B was a highly activated transcription factor by PG101 and played an important role in expression of the cytokines. These biological effectsdemonstrated that PG101 might have great potentials as a therapeutic immune modulator.
To understand the molecular mechanism(s) underlying the activation of cytokines by PG101, effects of the mushroom extract on production of cytokines were studied at the RNA and protein levels. Results from Northern blot analysis indicated that the steady-state RNA levels of TNF- and 7 other cytokines were highly increased in human PBMCs treated with PG101. The RNA level of TNF-, MIP-1, MIP-1, MIP-3 and IL-8 was not affected by the presence of cycloheximide, an inhibitor of the translation process, suggesting that they are the direct targets of PG101. A significantly high protein level of TNF-, MIP-1 and IL-8 remained detectable, even when cells were cultured with actinomycin D, 2 hrs prior to the PG101 treatment. These data indicate that PG101 controls selective cellular proteins, which play key roles in the innate Immune system, at the transcriptional and post-translational levels.

목차 Contents

• 제출문 ... 1
• 요약문 ... 2
• SUMMARY ... 4
• CONTENTS ... 6
• 목차 ... 7
• 제 1 장 연구개발과제의 개요 ... 8
• 제 1 절. 연구개발의 목적 ... 8
• 제 2 절. 연구개발의 필요성 ... 8
• 제 3 절. 연구개발의 범위 ... 11
• 제 2 장 국내외 기술개발 현황 ... 12
• 제 1 절. 국내 기술개발 현황 ... 12
• 제 2 절. 국외 기술개발 현황 ... 12
• 제 3 장 연구개발수행 내용 및 결과 ... 14
• 제 1 절. 연구개발의 목표 및 내용 ... 14
• 제 2 절. 실험방법 및 결과 ... 14
• 제 4 장 목표달성도 및 관련분야에의 기여도 ... 82
• 제 5 장 연구개발결과의 활용계획 ... 85
• 제 1 절. 연구개발의 기대효과 ... 85
• 제 2 절. 활용방안 ... 85
• 제 3 절. 추가연구의 필요성과 기업화 방안 ... 86
• 제 6 장 연구개발과정에서 수집한 해외과학기술정보 ... 87
• 제 7 장 참고문헌 ... 89
• 끝페이지 ... 92