보고서 정보
주관연구기관 |
(주)바이오피아 |
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 |
한국어
|
발행년월 | 2006-05 |
과제시작연도 |
2005 |
주관부처 |
농림부 Ministry of Agriculture and Forestry |
등록번호 |
TRKO201400023079 |
과제고유번호 |
1380003226 |
사업명 |
농림기술개발 |
DB 구축일자 |
2014-11-10
|
초록
▼
○ 연구결과
1. 홍삼의 성기능 관련 자료 수집 및 분석
- 성기능 관련 인삼 지표성분의 선정
2. 홍삼의 성기능 효능성분의 함량 극대화를 위한 가공 방법의 개발
- pH 및 온도 처리에 의한 유효성분 증량방법 개발
- pH 및 열처리 방법의 병용처리에 의한 유효성분의 증량방법 개발
3. 효능 발현 ginsenosides 성분의 분리 및 분석
- 사포닌의 분리
- 진세노사이드 Rg3성분 구조분석
4. 홍삼 가공처리물의 성기능 개선을 위한 적정량 설정
- 홍삼 가공처리물의 음경해면
○ 연구결과
1. 홍삼의 성기능 관련 자료 수집 및 분석
- 성기능 관련 인삼 지표성분의 선정
2. 홍삼의 성기능 효능성분의 함량 극대화를 위한 가공 방법의 개발
- pH 및 온도 처리에 의한 유효성분 증량방법 개발
- pH 및 열처리 방법의 병용처리에 의한 유효성분의 증량방법 개발
3. 효능 발현 ginsenosides 성분의 분리 및 분석
- 사포닌의 분리
- 진세노사이드 Rg3성분 구조분석
4. 홍삼 가공처리물의 성기능 개선을 위한 적정량 설정
- 홍삼 가공처리물의 음경해면체 평활근 수축ㆍ이완 실험
- 성 생활 및 사정 빈도와 정도 실험
5. 기능성 증대를 위한 한방소재 등 부재료 선정
- 한방처방, 기능성 소재 등 관련자료 수집 및 분석
- 적정 소재 선정
6. 홍삼 및 기능성 소재의 제품 원료화를 위한 가공방법 선정
- 특수 홍삼엑기스 제조의 표준화 및 규격화
- 한방소재 생약재의 추출 및 농축
7. 홍삼 가공처리물을 이용한 시제품 제조
- 시제품 제조를 위한 배합성분 및 비율 선정
- 시제품의 식미 효과 증진 방법 개발
- 향미 우수 실험구 선정
- 홍삼 가공처리물 엑기스와 부재료의 배합
- 식미효과 증진
- 시제품 생산 및 후살균
8. 홍삼 가공처리물과 한방소재 복합처방의 성기능 개선을 위한 투여 적정량 설정
- 2-4주 경구 투여 후 성기능관련 설문지 작성, 임상검사
- 혈중 성기능 관련 호르몬 수치 변화 측정
Abstract
▼
This project, "Mass production of high functional ginsenoside improving the sexual function using the processed red ginseng extract and its product development" was designed to develop its mass production method and confirmation of ginsenoside Rg3 for improving the sexual dysfunction by processed r
This project, "Mass production of high functional ginsenoside improving the sexual function using the processed red ginseng extract and its product development" was designed to develop its mass production method and confirmation of ginsenoside Rg3 for improving the sexual dysfunction by processed red ginseng extract, to develop the manufacturing method for improving sexual dysfunction using ginseng and red ginseng, and to confirm the clinical efficacy on the improving sexual dysfunction by ginsenoside Rg3 of processed red ginseng extract. The results of the research are summarized as follows :
1) Results on [develop its mass production method and confirmation of ginsenoside Rg3 for improving the sexual dysfunction by processed red ginseng extract]
(1) In order to enhance the components of bioactive ginsenosides and the manufacturing process of red ginseng, we developed the simplified method for red ginseng production. The red ginseng extract was prepared from red ginseng produced with the simplified method, and the production rate of extract (62°brix) was more than 60%.
(2) The ginsenosides of red ginseng were purified and analyzed by HPLC using ELSD. Ginsenoside-Rg3, Rh2 and Rh1 , specific artifacts found only in red ginseng, were detected by HPLC. Especially, contents of ginsenoside-Rg3 and Rh1 were detected high than two times in red ginseng produced the simplified method compared to commercial products.
(3) A single ginsenoside Rg3 was fractionate and its structure was analyzed by NMR.
(4) To increase the contents of functional ginsenosides by conversion, especially ginsenoside-Rg3 and Rh2, the extracts of red ginseng were treated with high temperature and citric acid or apricot extract.
(5) When the extracts were subject to 120℃ for 2 hours, the content of ginsenoside-Rg3 was increased 2 times than in control. When the extracts were subject to 120℃ and acidic conditions adjusted with citric acid, the ginsenoside-Rg3 was detected 2.8 times, but other ginsenoside were decreased heavily to 65%.
(6) When the extract were treated with for 12 hours at 80℃, the content of ginsenoside-Rg3 was increased to 3.3 times. Also, when the red ginseng extracts were treated with apricot extract, the ginsenoside-Rg3 was detected to 4 times than in control, but other ginsenoside were decreased lightly to 35%, not same as at the 120℃ treatment.
2) Results on [develop the manufacturing method for improving sexual dysfunction using ginseng and red ginseng]
(1) Data collection of sexual dysfunction pertinent and analysis of KRG : Collection and analysis of the research literature was performed data about improving sexual dysfunction and a reinforcement. The research data about sexual dysfunction improvement and a reinforcement from in effect of various KRG. Literature search it leads, selection the sexual dysfunction relation KRG index ingredient of KRG. Selection the representative sexual dysfunction relation index ingredient.
(2) Seven natural herbs using improving dysfunction since early times was extracted and concentrated with vaccum evaporator. Extract from seven herbs was harvested 35.32% yield. 20 g of extract was incubated in 37℃ incubator for bacteria and E. coli contamination. Any E. coli and bacteria was not observed onto petrifilm.
(3) The combination ratio of selected ingredients was determined. Vitamine B group(B1, 2, 3, 6) was supplemented for active sexual function and sucralose used for the sugar taste.
(4) Red ginseng extract treated with citric acid was not good for health dink because of its severe sour taste. Red ginseng extract treated with extract of Japanese apricot was also sour taste, but better than citric acid treated it. When malditol was added, its sour taste was decreased some degree. chicory
(5) To reduce the sour taste of drink, malic acid-sodium was supplemented, but the sour taste was not declined. Main ingredient for Placebo was used with chicory extract and supplemented with peppermint and coffee fragrance.
(6) To determine the period of circulation of produced drinks, growth of microorganism such as E. coli and bacteria was investigated for 3 weeks. There were no any microorganism in produced drinks
3) Results on [confirm the clinical efficacy on the improving sexual dysfunction by ginsenoside Rg3 of processed red ginseng extract]
(1) The Relaxation Contraction test of after KRGE : It executed a test from the research which KRGE processing material had effect on the relaxation contraction of Penis sponge body. We had administered the KRGE to the rabbit using feeding needle then checked the level of contraction and relaxation of corpus cavernosum penis. KRGE processing material was augmented the ingredients of ginsenoside g3 which had effect on high endothelium dependent relation and potassium channel open. It was resulted that Red ginseng group increased the blood pressure of corpus cavernosum penis, respectively 78.5±6.2, 105.0±10.2, 117.9±7.7 and 121.3±7.0 than control group during 1, 2, 4 and 6 weeks. The medication of processing red ginseng material was resulted that fill ratio of Penis sponge body increased rapidly. The fill ratio 0.025±0.00228 of control group increased to 0.02546±0.00317, 0.02816±0.00201, 0.0296±0.00327 and 0.02991±0.00282 during 1, 2, 4 and 6 weeks after administration respectively. It means that the fill of enis sponge body enhanced promptly as strong as long period time of administration. Comparing with lag time that reached at maximum blood pressure of corpus cavernosum penis. Lag time reached at maximum blood pressure of corpus cavernosum penis. Case 1, 2, 4, 6 week taking medicine hour of KRGE group, the hour when the control group which it takes medicine arrives to the maximum blood pressure of corpus cavernosum penis compared the 27.24±2.55 person place and the taking medicine duration route recording came to be short each with 25.25±2.28, 23.85±2.28, 23.62±3.25, 23.63±2.28.
(2) Sexual life and situation frequency and degree examination : It differed KRGE in the mouse and it took medicine and the mouse of the female it put in and the video monitor it used and the increase of the sexual life frequency which is the result consideration characteristic which observations the frequency and a degree of sexual life of 24 hour mouse was observationd. Little more from the hazard which gets the result which is reappearance characteristic currently while executing a repetition test, it is, the control group and it compares the frequency and a degree of ejaculation which it follows in taking KRGE and it is grasping an effect.
(3) General character of patients : There is no difference between control and KRGE group in year, tall and BMI.
(4) Effect of KRGE on sexual dysfunction : Surveys including libido, erection, ejaculation, sexual activity, and sexual satisfaction were given. Libido, erection, ejaculation, sexual activity, and sexual satisfaction were 3.4±1.3, 14.0±2.5, 4.8±1.3, 8.8±2.1, 7.2±1.9, respectively at 0 weeks. After KRGE administration, libido, erection, ejaculation, sexual activity, and sexual satisfaction were 5.8±2.2, 18.0±4.2, 7.4±2.7, 12.0±4.0, 9.0±2.8, respectively. In placebo, Libido, erection, ejaculation, sexual activity, and sexual satisfaction were 3.7±2.1, 15.0±3.6, 5.1±1.5, 10.0±3.0, 6.8±2.3. KRGE group was increased significantly compared to the control group. It was significantly increased by 49.0%, 62.7%, 53.6%, 54.6% and 60.1%, respectively, compared to control group.
(5) Change in serum testosterone by KRGE : The differences in the serum testosterone of the treated with KRGE and control were not significant at 0 week. In control group, there is no the difference between before and after treatment. After 4 weeks, there was tendency of better efficacy as compared to the group treated with 0 weeks of KRGE. Serum testosterone levels were found to be significantly higher in the C200 than the control group.
(6) Changes in serum biochemical parameters by KRGE : No significant changes were observed between all groups for total cholesterol, HDL-cholesterol, triglyceride, GOT, GPT, BUN, creatinine and glucose levels.
(7) Changes in rigidity and PSV and EDV by KRGE : Thickness, rigidity and angle of penis were found to increase in the KRGE-treated group. At 0 week, peak systolic velocity(PSV) was 22.3±7.4(left) and 27.9±6.1(right) and end diastolic velocity(EDV) was 11.9±4.0(left) and 8.1±3.1(right). After 12 week, PSV was 32.3±0.4(left) and 34.9±1.7(right). It was increased significantly compared to the control group. EDV was decreased significantly compared to the control group.
목차 Contents
- 제출문 ... 1
- 요약문 ... 2
- SUMMARY ... 12
- CONTENTS ... 17
- 목차 ... 19
- 제 1 장 연구개발과제의 개요 ... 21
- 제 1 절 연구개발의 목적 ... 21
- 제 2 절 연구개발의 내용 및 범위 ... 22
- 1. 1차년도 ... 22
- 2. 2차년도 ... 23
- 제 3 절 연구개발의 필요성 ... 24
- 제 2 장 국내 외 기술개발 현황 ... 25
- 제 1 절 국내 기술개발 현황 ... 25
- 제 2 절 국외 기술개발 현황 ... 26
- 제 3 절 국내 외 기술개발현황에서 차지하는 위치 ... 26
- 제 3 장 연구개발수행 내용 및 결과 ... 29
- 제 1 절 홍삼의 성기능 효능성분(ginsenoside Rg3)의 함량증대를 위한 가공방법의 개발 ... 29
- 1. 서설 ... 29
- 2. 재료 및 방법 ... 32
- 3. 연구결과 및 고찰 ... 35
- 제 2 절 홍삼을 이용한 성기능 개선 효능을 갖는 기능성제품 제조기법의 개발 ... 48
- 1. 부소재 선정을 위한 한약 소재 등 관련자료 수집 및 분석 ... 48
- 2. 한방처방 부소재의 추출 및 농축 ... 53
- 3. 시제품 제조를 위한 배합성분 ... 53
- 4. 시제품 및 위약균의 식미 효과 증진 방법 개발 ... 54
- 5. 시제품 생산 및 안전성 검사 ... 55
- 제 3 절 홍삼 가공처리물의 지표성분 성기능 개선을 위한 효능확인 ... 57
- 1. 홍삼 성기능 관련 자료 수집 및 분석 ... 57
- 2. 재료 및 방법 ... 59
- 3. 결과 및 고찰 ... 63
- 제 4 장 목표달성도 및 관련분야에의 기여도 ... 76
- 제 1 절 연구개발목표의 달성도 ... 76
- 제 2 절 관련분야의 기술발전에의 기여도 ... 77
- 제 5 장 연구개발결과의 활용계획 ... 78
- 제 1 절 추가연구의 필요성 및 타 연구에의 응용 ... 78
- 제 2 절 기업화 추진방안 ... 78
- 제 6 장 연구개발과정에서 수집한 해외과학기술정보 ... 79
- 제 7 장 참고문헌 ... 81
- 끝페이지 ... 92
※ AI-Helper는 부적절한 답변을 할 수 있습니다.