보고서 정보
주관연구기관 |
성균관대학교 SungKyunKwan University |
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 |
한국어
|
발행년월 | 2002-11 |
주관부처 |
농림부 Ministry of Agriculture and Forestry |
등록번호 |
TRKO201400024017 |
DB 구축일자 |
2014-11-29
|
초록
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○ 연구개발 목표 및 내용
1) 히어리의 자생지 특성
2) 히어리의 신품종육성
3) 히어리의 번식방법 및 분재활용
4) 조직배양을 이용한 대량증식법 개발
○ 연구성과
1) 히어리의 자생지 특성 - 韓國 特産 히어리의 自生 群落地는 경남 산청군 지리산 지역, 경남 남해군 상주면 금산지역, 경기도 포천군 백운산 지역에서 각각 발견되었다. 韓國 特産 히어리의 自生地는 계곡을 중심으로 넓게 분포하고 있었으며 아교목층과 灌木層에 주로 분포하였다. 또한 히어리 群落地는 히어리만 집단적으로 발생하였고 맹아지가 많
○ 연구개발 목표 및 내용
1) 히어리의 자생지 특성
2) 히어리의 신품종육성
3) 히어리의 번식방법 및 분재활용
4) 조직배양을 이용한 대량증식법 개발
○ 연구성과
1) 히어리의 자생지 특성 - 韓國 特産 히어리의 自生 群落地는 경남 산청군 지리산 지역, 경남 남해군 상주면 금산지역, 경기도 포천군 백운산 지역에서 각각 발견되었다. 韓國 特産 히어리의 自生地는 계곡을 중심으로 넓게 분포하고 있었으며 아교목층과 灌木層에 주로 분포하였다. 또한 히어리 群落地는 히어리만 집단적으로 발생하였고 맹아지가 많이 발달하였다.
2) 히어리의 신품종육성 - 히어리의 자생지에서 생육기간중 잎이 자주색인 新品種으로 C. coreana 'SKK 1', 'SKK 2', 'SKK 3', 그리고 葉色이 노란색인 C. coreana 'SKK 4'가 각각 選拔되었다. 종자파종된 실생묘에서 단풍색이 아름다운 新品種으로 노란색의 단풍이 드는 C. coreana 'SKK 5'와 빨간 색의 단풍이 드는 C. coreana 'SKK 6'이 각각 選拔되었다. 또한 自生 히어리의 種子 파종된 1年生 實生苗중 조기 開花가 유도되는 早生種 品種 C. coreana 'SKK 11', 'SKK 12', 'SKK 13', 'SKK 14'가 각각 選拔되었으며, C. coreana 'SKK 13'은 꽃에서 향기가 나는 특성이 있었다. 선발된 계통들은 接木을 통한 後代檢定이 실시되었으며 後代에서도 開花가 유도되어 母本의 특성이 後代에도 나타나는 것을 증명하였다. 자연방임수분된 中國히어리 實生苗에서 꽃에서 향기가 나는 系統 C. sinensis X 'SKK 31', 'SKK 32', 'SKK 33', 'SKK 34', 'SKK 35', 'SKK 36'이 選拔되었다.
3) 히어리의 번식방법 및 분재활용 - 자생 히어리의 종자 번식은 종자 채취 후 건조시키지 않고 3개월 저온 처리를 실시한 처리구에서 80% 이상의 높은 발아율을 보여 가장 효과적이었다. 히어리속식물의 삽목번식은 경지삽목과 녹지삽목 공히 저농도의 IBA에서 발근이 효과적이었으며 뿌리의 생장 역시 양호하였다. 히어리의 분재는 C. spicata를 이용하여 크게 대작품과 중작품으로 구분되었으며 분재수형은 곧은 줄기형과 쌍간 줄기형으로 구분되었다.
4) 조직배양을 이용한 대량증식법 개발 - 히어리나무의 액아배양으로 유시, 성숙목의 대량번식이 가능함을 보여주었으나 선발개체의 효율적인 기내번식을 위해서는 재유령화의 기술개발과 토양순화율을 증진시켜야 하는 것으로 나타났다.
○ 연구성과 활용계획(실적)
- 논문발표 실적 : 1) 2002년 : “Genetic resources for new cultivar breeding of selected Asian ornamentals” - 26회 국제 원예학회 발표, Acta Horticulturae에 게재예정
2) 2001년 : 한국 특산 히어리(Colylopsis coreana)와 히어리속 식물(Colylopsis spp.)의 형태적 특성 비교 - 한국 조경학회지 추계학술발표회 118-120
Abstract
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1. Ecological Characteristics and Development of New cultivars of Korean Native Corylopsis coreana
This study was carried out to investigate the natural distribution, growth characteristics, and propagation methods of Corylopsis coreana and develop new varieties with yellow or red leaf color, ear
1. Ecological Characteristics and Development of New cultivars of Korean Native Corylopsis coreana
This study was carried out to investigate the natural distribution, growth characteristics, and propagation methods of Corylopsis coreana and develop new varieties with yellow or red leaf color, early flowering, beautiful fall leaf color of C. coreana for landscape woody plants. Korean native Corylopsis coreana is found from Mt. Ji-ri in Sanchung of Kyungnam, Mt. Kum-san in Namhae of Kyungnam, and Mt. Baek-un in Pochun of Kyunggi. They are widely distributed in the sub-tree layer and shrub layer around the ravine. In the root diameter class distribution, Mt. Kum-san widely distributed trees with 2∼3cm root diameter. many adult trees with 7cm diameter widely planted in the Mt. Ji-ri of San-chung. In addition, there are planted trees with 3∼5cm of root diameter, 4∼6m of tree height in Mt. Baek-un, Kyunggi. In the morphological characteristics such as leaf, flower, flowering, and winter bud of Korean native C. coreana according to native sites were not different from those of 2-year-old grated plants from native sites, San-chung, Kyungnam, Nam-hae, Kyungnam, and Mt. Baek-un, Kyunggi. In the Corylopsis species, C. coreana, C. willmottiae, and C. vetchiana had larger flowers, longer flower cluster, more flowers than other species. C. sinensis and C. veitchiana had fragrant flowers. In addition, species with beautiful leaf color were C. spicata 'Yellow spring' with yellow color and C. willmottiae 'Spring purple' with purple color, which eventually changed to green. The winter bud of C. sinensis, C. willomottiae, C. willmottie 'Spring Purple', and C. vechiana was oval and then different from that of other species. Comparing of the morphological characteristics among C coreana, C. spicata, and C. sinensis, C. coreana had earlier flowering dates and brighter yellow flowers than those of other species. Flower cluster of C. sinensis was shortest, but it had most number of flowers and fragnance. In addition, winter bud of C. coreana and C. spicata with elliptical shape were different from that of C. sinensis with oval shape. New cultivars with red leaves, C. coreana 'SKK 1', C. coreana 'SKK 2', and C. coreana 'SKK 3' were selected from seedlings of Korean native C. coreana, which had purple shoots that eventually changed to green. And C. coreana 'SKK 4' had yellow leaves during summer. New selected cultivars of C. coreana showed successful union by grafting and inherited mother characteristics. New cultivars with beautiful fall leaf color, C. coreana 'SKK 5' with yellow color and C. coreana 'SKK 6' with red color were selected from seedlings of Korean native C. coreana. And then this useful characters were still inherited after cutting. In the open pollinated seedlings of Korean native C. coreana, C. coreana 'SKK 11', C. coreana 'SKK 12', C. coreana 'SKK 13', and C. coreana 'SKK 14' were selected as new cultivar with early flowering, which flowered on the 1-year-old tree. New cultivar with early flowering, C. coreana 'SKK 13', had also fragrant flowers. New selected cultivars of C. coreana showed successful union by grafting and inherited mother characteristics. In addition, strains with fragrance, C. sinensis X 'SKK 31', C. sinensis X 'SKK 32', C. sinensis X 'SKK 33', C. sinensis X 'SKK 34', C. sinensis X 'SKK 35', and C. sinensis X 'SKK 36', were selected from open pollinated seedlings of C. sinensis.
2. Propagation and Utilization of Bonsai of Korean Native C. coreana.
Freshly collected seeds of Korean native C. coreana was over 80% of germination in the 3 month cold stratification period and had vigorous shoot length. In the hardwood cutting, rooting percentage of Korean native C. coreana was over 70% in the all treatments, especially highest at 1,000ppm. However, the percentage of softwood cutting was highest as 60% in the 5,000ppm of IBA. Therefore, the most effective method for rooting of C. coreana was treatment with 1,000ppm IBA by hardwood cutting, which showed rooting rate of over 90%. Flowering of Korean native C. coreana in the 3-year-old seedlings was over 90%. However, 1-year-old cuttings showed 70% of flowering rates and 2-year-old cuttings had 95% flowering.
3. Mass Propagation of Korean Native C. coreana in Vitro.
We have developed an in vitro micropropagation system using nodal segments of Corylopsis coreana. Explants from both juvenile tree (one-year-old greenhouse stock seedlings) and mature tree (ten-year-old tree in nursery) were compared with regard to propagation efficiency. Combinations of BA and zeatin were effective on shoot proliferation since the best result was obtained on MS medium supplemented with 0.5∼3.0mg/L zeatin and 0.2mg/L BA. Generally, juvenile explants were better in both shoot proliferation and growth than mature explants. However, within six months after culture initiation the mature explants also produced three shoots per explant. Distinctive differences in rooting and adaptability to soil were observed by mother trees. Whereas shoots originated from juvenile explants rooted as high as 97%, those from adult explants showed 62% rooting. Similar result was also observed in soil acclimatization in that while the plantlets derived from juvenile plants survived 67%, only 48% of those from adult trees survived. The results showed a possibility of using tissue culture for the micropropagation of Corylopsis coreana. In addition, the advance of the research would be conducted for acclimatization in mature trees for practical application.
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