초록 ▼

○ 연구결과
1-23조합의 교잡 후대계통에 대한 계통육성 계속
-육성 계통 중 최우수 6개 계통은 선발
-육성과정 중에 발견한 돌연변이 계통은 우수성이 인정되어 계속하여 육성중
-기존 아가콩과 육성중인 계통을 이용하여 수종의 기능성 제품을 개발
2-아가콩 3호를 이용한 미생물(Aspergillus sojae)을 이용한 경우 이소플라본을 ~10,500ppm까지 증대시켰으며, 첨가제(피니톨)를 사용한 경우 ~12,500ppm정도로 30%이상의 이소플라본 증대조건을 확립하였음
-특히 배당체 이소플라본을 비

○ 연구결과
1-23조합의 교잡 후대계통에 대한 계통육성 계속
-육성 계통 중 최우수 6개 계통은 선발
-육성과정 중에 발견한 돌연변이 계통은 우수성이 인정되어 계속하여 육성중
-기존 아가콩과 육성중인 계통을 이용하여 수종의 기능성 제품을 개발
2-아가콩 3호를 이용한 미생물(Aspergillus sojae)을 이용한 경우 이소플라본을 ~10,500ppm까지 증대시켰으며, 첨가제(피니톨)를 사용한 경우 ~12,500ppm정도로 30%이상의 이소플라본 증대조건을 확립하였음
-특히 배당체 이소플라본을 비배당체 이소플라본으로 전환하는 실험을 통하여, genistein 및 deidzein의 함량을 9,000ppm까지 증대 시켰으며 이는 배당체로 환산할 경우 14,400ppm이 되는 높은 함량 증대효과를 거두었으며, 이는 생체 흡수율을 고려하면 엄청난 효과를 거둘수 있음
-스트레스 조건하에서 daidzein의 이차대사 산물인 pterocarpan을 prep LC 및 컬럼 크로마토그래피를 이용하여 순수하게 분리하였음
-위에 얻어진 pterocarpan의 다양한 활용도를 찾는 목적으로 이들의 미백 기능성을 확인하였으며 향후 이를 이용한 다양한 기능성 화장품제조에 이용할 것임
3-콩 유전자원 519종의 검정을 통해 고지방 자원 3종, 고 oleic 4자원 및 저 linoleic 10종의 양질지방산 자원 14종 발굴
-CS27002 조합합 등 15조합은 F1세대에서 60개체를, CS27001 등 5조합은 우량계통 113개체와 SSD방법에 의하여 1492립을 선발하였음
-고지방자원인 동농46(25.5%), 고 oleic 자원인 KLG12073(56.0%) 및 12074(56.0%) 및 저linolenic자원 KLG12073(3.4%) 및 MS382 (2.9%)는 환경변이가 작고 안정적임
-고지방, 양질 지방산 자원 20종의 종실, 두유 및 두부의 지방, 지방산, 단백질, 11S/7S 비율, 무기성분 및 phytate 함량의 분석을 통하여 가공적성을 평가하였음
4-아가콩의 isoflavone 함량은 재배지역, 시비 수준, 대사조절물질 등에 의해 크게 차이가 나타났다. 따라서 아가콩의 isoflavone 함량이 높은 기능성 콩을 생산하기 위해서는 특산지 조성과 재배기술의 개발이 필요하며 이를 이용한 기능성 콩 제품의 개발에도 응용할 수 있을 것으로 사료됨.
-친환경적 아가콩 재배를 위해서는 답전윤환을 통해 잡초군락을 줄이고 이랑에 검정비닐피복과 고랑에 피복작물의 부산물을 피복함으로써 제초제의 사용을 피할 수 있으며, 고랑의 잡초방제를 위해 잡초억제물질 생성균류를 선발하여 피복작물 부산물과 혼합처리하는 것이 효과적일 것으로 사료됨.
5-아가콩에서는 세균성점무늬병(Pseudomonas savastanoi pv. glycinea)은 8월 이후에, 점무늬병(Ascochyta glycines)과 자주무늬병(Cercospora kikuchii)은 9월 이후에 심하게 발생되었으며 아가콩의 세균병 및 바이러스병에 대한 저항성은 청자 및 대원콩과 같은 수준인 것으로 생각됨.
-이들의 방제를 위해 고시된 3종의 살균제 생물농약을 이용하여 아가콩 포장에서의 방제력을 시험한 결과 처리별 통계적인 유의차는 인정되지 않았다. 다만 푸른세상(활성미생물: Trichoderma harzianum)을 처리한 구에서는 아가콩의 증수효과가 2,220g이었으며, 무처리구에서는 1,980g이었다. 아가콩에서 발견된 해충으로는 노린재류의 발생이 가장 많았으며 그중에서도 풀색노린재(Nezara antennata)와 톱다리개미허리노린재(Riptortus pedestris)의 밀도가 가장 높았다. 청자콩과 대원콩과의 비교에서는 아가콩에서 노린재류의 발생이 상대적으로 적었음.
-톱다리개미허리노린재 천적의 증식을 위해 기주알의 저온저장 기술을 이용하였고 약 2°C에서의 30일 동안 냉장 보관 후에는 노린재알이 부화하지 않았으며, 이들 알에서 천적인 노린재깡충좀벌(Ooencyrtus nezarae)과 노린재검정알벌(Gryon japonicum) 모두 부정적인 영향을 받지 않고 성공적으로 발육 및 성장을 완료하였다. 또한 두 기생벌 개체군의 상호작용에도 영향이 없었다. 또한 저온저장한 톱다리개미허리노린재의 알 방사가 노린재의 기생률 향상 및 밀도 억제에 효과가 있는 지를 경북 안동시 송천동 소재의 아가콩 재배지(45m×26m)를 조성하여 시험한 결과 노린재의 알,약뭉 및 성충의 밀도 억제효과는 미미하였으나, 노린재검정알벌의 기생율 증가 효과는 있었음.
-따라서 노린재류의 천적의 보존을 통한 생물적 방제를 위해 노린재의 저온저장 알 방사법이 유효할 것임.
6-Glyceollin I은 골아세포에서 Runx2 단백질의 분해를 촉진시키고, Rankl의 mRNA 수준을 감소시켜 파골세포의 분화를 억제하고, 고용량의 비타민 D의 투여에 의해 유도된 골다공증 동물모델에서 골다공증 증상을 크게 완화시킴.
-Genistein, daidzein과 glyceollin I은 여러 가지 수축제로 알려진 KCl, NaF, U46619,phenylephrine, 그리고 PDBu에 반응하는 혈관 수축을 낮추었고, 이러한 혈관 수축 효과는 RhoA/Rho kinase 신호 전달 경로를 억제하여 혈관 수축을 약화시켰음.
7-아가콩은 기존의 콩보다 isoflavone의 함량이 약 4배 이상 높았고 항산화 활성, 암예방 활성, 항암활성 모두 우수한 활성을 보여주었음.
-특히 항산화 활성이 강하게 나타났고, 항산화 활성물질 구명연구에서 genistin과 daidzin이 활성물질로 분리, 동정되었다. 또한 4종의 활성분획물을 제조하였다. 활성분획물 Bu3은 단일화합물보다 높은 활성을 보여주는 synergy effect가 있었다. 또한 패턴분석 결과 아가콩에는 주요 이소플라본뿐만 아니라 타 품종에서는 검출되지 않는 미량 성분들도 많이 검출되어 추후 미량성분에 대한 연구가 필요할 것으로 사료됨.
8-아가3호, 4호 품종과 일반 콩으로 제조한 청국장의 항산화 활성을 비교하였을 때, 아가 4호가활성이 높았으며, 사염화탄소로 유도되는 간독성 및 신장독성 완화효과도 높은 것으로 나타났음.
9-글라이세오린을 이용하여 항암 및 항아토피 활성을 검증한 결과 wound assay를 이용하여 암세포의 운동성을 저해하는 것을 확인 할 수 있었으며, 마우스를 이용한 아토피 동물 모델을 이용하여 항아토피 활성이 있는 것을 알 수 있었음.
-콩의 주요한 유효성분인 피니톨과 이노시톨을 이용한 생리황성 물질의 확인 결과 피니톨은 세포의 운동성을 저하시키고, 항암분자마커인 ICAM-1, CD44, MMP17, MMP14, ARF2의 발현을 감소시키는 것으로 나타남.
-산업화와 관련된 콩의 유효물질에 대한 연구는 지속적으로 이루어지고 있으며, 특히 피니톨은 상품의 가치를 인정받아 다수 생산되고 있음.
10-GC-MS, LC-MSMS를 이용한 콩의 기능성 물질 분리
-산업화 가능한 기능성 물질의 품종별 평가
-1차년도에서 평가된 물질을 중심으로 대량 분리 및 정제, 농축기술 기발
-공장형 분리, 정제 농축 기술 개발
-기능성 물질을 이용한 레시틴 제품 개발
-기능성 물질을 이용한 두유 제품 개발
11-콩으로부터 루나신강화추출물(LES)을 분리하고, 이에 대해 항산화 및 세포손상억제 효능 분석함.
-루나신 처리에 의해 산화적 DNA 손상억제, elastase의 활성 억제, DPPH free radical 소거능 및 화학적 발암물질에 의해 야기되는 세포의 비정상적인 생육을 억제함.
-Lunasin은 UVB에 의해 야기되는 elastin의 degradation을 억제함으로써 photo-aging을 억제하고, 알레르기 접촉피부염을 유도한 동물실험에서 LES 피부도포시 표피두께의 증가가 억제되는 것을 확인함.
-콩의 대표적 이소플라본인 Genistein은 농도 의존적으로 암세포 HCT116의 생존율을 감소시켰으나, daidzain은 세포생존율에 큰 변화가 없었다. Genistein과 Gaidzein의 병합처리에 의한 상승적인 세포 독성효과가 발견되었으며, 이는 항암유전자인 NAG-1 유전자의 과대발현과 부분적으로 관련이 있다고 생각된다. Genistein은 마우스 전지방세포 3T3-L1 세포주의 생존율 및 분화를 억제하였으며, Sirt-1 유전자의 발현이 억제됨이 관찰되었음.
12-본 연구에서는 콩을 원료로 하여 다양한 제품 및 그 기능성을 규명하였다. 혈전용해효소를 분비하는 균주를 선별하여 기능성 육종콩을 발효하여 발효한 콩추출물을 이용하여 항당뇨, 항암, 항산화 등 다양한 기능성을 검정한 결과 기존의 콩 발효물에 비하여 높은 활성을 나타내었음.
-콩에 함유된 아이소플라본, 안토시아닌, 제니스틴을 이용하여 면역활성, 항암등의 활성을 확인해 본 결과 높은 활성을 나타냄.
-콩에 함유된 사포닌 성분을 분리 정제하여 이를 이용한 비만억제 식이음료를 개발하여 항비만활성을 살펴보는 등의 많은 기능성을 확인함.
13-공복혈당, GOT, GPT, 중성지방, 콜레스테롤 등 유의적 감소를 보였음
-시제품 디자인 및 제품 개발 완료

Abstract ▼

Ⅳ. Results and suggestions
○ To improve the demerits of Aga lines such as easy lodging, low yield, and some viny habit and develop better lines in use than Agakong, 23 cross combination were made. Those crosses are under generation advancement by breeding method of SSD and conventional pedigree o

Ⅳ. Results and suggestions
○ To improve the demerits of Aga lines such as easy lodging, low yield, and some viny habit and develop better lines in use than Agakong, 23 cross combination were made. Those crosses are under generation advancement by breeding method of SSD and conventional pedigree one.
○ Two thousands NILs of Aga 3, and 4 were screened for isoflavone content including of major agronomic characteristics in the last three years and total of six promising lines were selected. Total of six lines, two in 2009 and 4 in 2010, were applied for the registration of "certificate on grant of plant variety right".
○ Out of applied lines, Aga 5 is known to have the highest isoflavone content among all black soybean varieties ever known in the world; Aga 6 is to be believed to have better lodging resistance and high yielding compared with Aga 3; Aga 7 and 9 are known to have the highest daidzein(-in) contents; and Aga 9 and 10 are known to have the highest genistein(-in) content out of those lines of yellow and black seed coat, respectively.
○ A new Agakong population having tawny pubescence and black seed coat was developed from Aga population of gray pubescence and yellow, green, and greenish yellow seed coat. Those lines believed to be resulted by reverse mutation in seed coat showed extremely high isoflavone content. In general, lines of black seed coat show more or less 60% of isoflavone content of yellow and green ones. It is assumed that the newly formed black seed coat lines were developed from the population developed by pedigree method which screened isoflavone content every generation. We hope that several lines having more than 10 times of isoflavone content than current leading black soybean varieties can be selected from the newly developed population within a few years.
○ When we use microorganism(Aspergillus sojae) with Agar Ⅲ, we could increase isoflavone up to ～10,500 ppm, in case of using additional materials(e.g. pinitol), we secured 30% above of increase condition of isoflavone up to ～12,500 ppm.
○ Through the test that isoflavone(glycoside) especially changed into non-glycoside, we could increase genisein and deidzein contents up to 9,000ppm. When we converse them into glycoside rate, they showed up to 14.400ppm.
○ We separated pterocarpan, which is a secondary metabolite of daidzein, by Prep LC and column chromatography under the stress condition.
○ We verified whitening function while confirming of various application of pterocarpan.
○ As pre-conditioning of bean roasting, roasting at 250℃ for 30 minutes showed good flavor and taste.
○ The masking using of 2nd additional materials were not necessary in making of soybean tea by the use of roasted Aga soy-extracts( solvent ratio of 1:100), because the extract itself showed very good penal scores.
○ The highest daidzein content, 11.64 μg/ml, was obtained at 99.55℃, 106.19 minutes while 9.61 μg/ml was obtained at extraction temperature of 99.50℃ and extraction time of 103.23 minutes for genistein.
○ The selling products of 340ml bottle contained more or less 30mg isoflavone content which is evaluated as very good functional drink.
○ Agakong powder was made by roasting of 30 minutes and sieving with 30 mesh. The best Macgori was made when 0.3-0.4% Agakong powder of all source materials was added at the stage of 2nd adding one during the fermentation.
○ The optimum amount of Saesongee(mushroom) powder for enhancing of the first feeling of drinking was about 2% of all source materials and the best adding stage of mushroom powder was the first adding one.
○ The selling product(Takjoo) of 740ml bottle contained about 30ml isoflavone content with good drinking feeling.
○ The developed cosmetics in the project have basic concepts containing of especially much genistein which is known to have the effect of anti-oxidant and improving of wrinkles by protecting of DNA damage. At the same time, they are known to be pro-human products via natural emulsification, low stimulator, and affinity with membrane of living cells by use of soy-lecithin.
○ The experimental products would be tested for collagen synthesis assay and improvement of wrinkles by the use of CCD-986sk, HS68, Detroit 5116 cell. At the same time, more experiment would be done such as collagenase inhibition assay for improving of wrinkles.
○ A total of 379 soybean genetic resources including 128 Korean, 214 Chinese and 37 American varieties were used to evaluate oil content and fatty acid composition in 2007. Seed oil content of 379 genetic resources ranged from 12.7% to 26.0% with an average of 19.1%, and oil content was mostly distributed in range of 18.0% and 20.0%. Three resources were higher than 24% of oil content.
○ Palmitic and Stearic acid contents of 379 soybean genetic resources ranged 4.1∼18.1% and 1.9∼12.7% with an average of 10.6% and 2.9%, respectively. Oleic, linoleic and linolenic acid contents ranged from 15.3 to 56.0%, from 24.4 to 68.1% and from 3.4 to 11.1% with an average of 28.2%, 51.2% and 7.1%, respectively. And saturated and unsaturated fatty acid contents ranged from 6.7 to 21.7% and from 78.3 to 93.4% with an average of 13.5% and 86.5%, respectively.
○ A total of 140 Japanese genetic resources were used to evaluate oil content and fatty acid composition in 2009. An average oil content was 17.2%.
Oleic, linoleic and linolenic acid contents ranged from 16.6 to 55.2%, from 25.8 to 59.1% and from 3.0 to 12.0% with an average of 26.1%, 51.9% and 8.9%, respectively. Among Japanese genetic resources, two resources, 1-116 and 1-112, contained higher oil content than 22%.
○ Three Chinese resources, Shuinong 14(26.0%), Dongnong 434(25.7%) and Dongnong 46(25.0%) were considered to be prominent resources for breeding of new soybean with high oil content. And six resources containing high oleic acid content, KLG12073(56.0%), KLG12074(56.0%), Jilin 14(52.2%), M23(55.2%), Kwangankong (55.9%) and Bongeui(50.9%), and 9 resources including KLG12093(3.4%), KLG12096 (3.5%) and MS382(2.9%) containing low linoleic acid content were considered to be prominent resources for the breeding of new soybean varieties with fatty acid composition of high quality.
○ A total of 20 combinations were crossed in 2007, and five combinations were selected and developed breeding lines for F1 and F2 generations in 2008 and 2009, respectively. 113 prominent lines were selected in five combinations of F2 generation, and also, a total of 1,492 seeds including 252 seeds of CS27001 combination, 352 seeds of CS27004 combination, 140 seeds of CS27006 combination, 196 seeds of CS27114 combination and 552 seeds of CS27017 combination were selected by single seed descent (SSD) breeding method for using bulk breeding.
○ The other 15 cross combinations were advanced for F1 generation in 2009, and 60 lines were selected in the same year. Generation advance of breeding lines developed in this study will be carried out continually for the selection of prominent lines.
○ Experimental resources showed an average oil content of 19.1% and 20.7% in 2007 and 2008, respectively. Palmitic acid contents in both 2007 and 2008 were not significantly different. Oleic and linolenic acid contents were higher in 2007 than in 2008, but stearic and linoleic acid contents were higher in 2008 than in 2007.
○ An average oil content of Dongnong 46 showed the highest content in two years (2007 and 2008). KLG12073 showed the highest content of oleic acid with an average of 51.0%, and KLG12080 showed the highest content of linoleic acid with an average of 66.2% in two years. KLG12093 and KLG12096 showed the lowest content of linolenic acid with an average of 3.4% and 3.5% in two years, respectively.
○ Oil, palmitic and stearic acid contents were not significantly different by different planting dates. However, oleic, linoleic and linolenic acid contents were different by planting dates.
○ Oleic acid content of KLG12074 and Daepungkong showed the highest and the lowest, regardless of planting dates, respectively.
○ According to the difference of cultivation region, the highest and the lowest oil contents were recorded in Muan and Yeoncheon, respectively.
Oleic acid content showed the highest in Cheongju and the lowest in Daegu. Linoleic acid content showed no significant difference by cultivation regions. However, linolenic acid showed higher content in Daegu and Yeoncheon than in Cheongju and Muan.
○ Oleic acid contents of KLG12074 and KLG01397 showed the highest by different cultivation regions. KLG12081 showed the highest content of linoleic acid and the lowest content of linolenic acid.
○ Oil content of soy milk and tofu in 20 varieties ranged from 17.1 to 24.6% and from 0.6 to 1.9%, respectively. Varieties with high oil content showed positive correlation with oil content of soy milk, but showed negative correlation with oil content of tofu. Varieties with high fatty acid content showed positive correlation with oil contents of soy milk and tofu.
○ Dongnong 98-14 and KLG12088 showed the highest and the lowest oil content of seed, respectively. Bongeui and KLG12081 showed the highest and the lowest oleic acid content of seed, respectively. KLG12081 and KLG12073 showed the highest and the lowest linoleic acid content of seed, respectively. KLG12073 and KLG12096 showed the highest and the lowest linolenic acid content of seed, respectively.
○ Hefeng 95-873 and Danbaegkong showed the highest and the lowest oil content of soy milk, respectively. KLG12074 and KLG12081 showed the highest and the lowest oleic acid content of soy milk, respectively.
KLG12081 and KLG12073 showed the highest and the lowest linoleic acid content of soy milk, respectively. Heinong 44 and KLG12096 showed the highest and the lowest linolenic acid content of soy milk, respectively.
○ Shihoudadou and Danbaegkong showed the highest and the lowest oil content of tofu, respectively. KLG12074 and KLG12088 showed the highest and the lowest oleic acid content of tofu, respectively. KLG12088 and KLG12073 showed the highest and the lowest linoleic acid content of tofu, respectively. Bongeui and KLG12081 showed the highest and the lowest linolenic acid content of tofu, respectively.
○ The isoflavone contents of Agakong were greatly affected by growing areas, fertilizer levels, and metabolism regulating elicitor such as SA, ASA, Chitosan. Therefore, in order to produce highly functional soybean a specific area should be selected and cultivation techniques developed to increase isoflavone content could be applied for production of functional soybean.
○ For the environment friendly cultivation of Agakong, weed population should be reduced by paddy field-upland rotation system and black plastic cover and by-products of cover crop could be used in the ridge and the furrow to avoid the use of herbicides. For weed management in the furrows, endophytic fungi with an inhibiting activity selected from rhizosphere of weeds could be applied with by-products of cover crop to reduce weed occurrence.
○ Riptortus pedestris and Nezara antennata was the most abundant stink bug species on all the tested soybean varieties. The occurrence of R. pedestris eggs and nymphs were not significantly different among the three varieties. However, the seasonal density of R. pedestris adults was lower on Agakong than on Cheongjakong.
○ Agakong was infected by Pseudomonas savastanoi pv. glycinea, Ascochyta glycines, and Cercospora kikuchii and found to be susceptible in similar degree with the other two varieties. Three environmentally sound disease management agents, i. e., Trichoderma harzianum, Bacillus subtilis, and Paenibacillus polymyxa, were evaluated for the control efficacy in Songcheon, Andong in 2008. There was no treatment effect, although Trichoderma harzianum treated plot produce 2,220g meanwhile untreated control plot produced 1,980g seeds.
○ Host egg refrigeration did not affect host acceptance behavior of Gryon japonicum and Ooencyrtus nezarae on preparasitized eggs or larval competition between the two parasitoids in multiparasitized refrigerated host eggs. Therefore, host egg refrigeration may not interrupt biological attributes of the parasitoids.
○ we conducted an experiment of cage-exclusion design to verify the former results that release of non-viable refrigerated eggs of R. pedestris is known to enhance natural parasitism in soybean fields in more manipulative approach in a Agakong field located at Songcheon, Andong in 2008. We found significantly higher parasitism by G. japonicum on the eggs collected from treated plots compared to the control plots. It is verified that releasing non-viable eggs of R. pedestris help to enhance natural parasitism in soybean field.
○ Glyceollin inhibited Runx2 protein stability, alkaline phosphatase activity and Alizarin Red S staining. Glyceollin also inhibited osteoclastogenesis by inhibition of TRAP staining.
○ In vivo osteoporosis mouse model, glyceollin showed a protective effects by injection of calvaria compared to wild type. Glyceollin strongly inhibited RANKL expression without big fluctuation of osteoprotegerin. Therefore, RANKL/Opg ratio was strongly inhibited by glyceollin, which makes a antiresorptive effect on bone resroption in mouse model. Glyceollin has a strong anti-osteoporotic effect by inhibition of bone turnover rate.
○ Genistein, daidzein or glyceollin I reduced vascular contraction induced by KCl, NaF, U46619, phenylephrine, or PDBu. They exert vascular relaxation, at least in part, through inhibition of the RhoA/Rho kinase pathway. K+ channels were not involved in vascular relaxation induced by glyceollin I.
○ Genistein, daidzein or glyceollin is a good candidate to develop as a therapeutic drug for osteoporosis and cardiovascular diseases.
○ Biologically active compounds of Aga bean were isolated by continuous column chromatography and were identified as genistin and daidzin, respectively using FAB-MS, 1H-NMR, and 13C-NMR analysis. Among active fractions, EA3 has showed the most potent anticancer effects in HepG2 and MCF7 cells. Active fractions, Bu-3 showed synergy effect.
○ One of the objectives of the study was to develop cheonggukjang (Korean natto) using Aga 3 or 4 variety of soybean which are extraordinary high in isoflavone content. As a first step toward functional cheonggukjang development, six kinds of Bacillus strains were evaluated for the properties of isoflavone metabolism and antioxidant activity of the product. Bacillus liqueniformis 86-1 turned out to be one of best strains for preparing cheonggukjang. Cheongukjang prepared with Aga 4 showed the highest protective effect against carbon tetrachloride-induced hepatotoxicity in mouse model, suggesting that cheonggukjang made of Aga 4 had good antioxidant capacity and deserves further endeavor for commercialization. It was also demonstrated that the functional cheonggukjang could successfully be applied to formulation of salad dressing and spread within 1% of total solid.
○ Glyceollin using antitumor activity by wound assay and have anti-atopic activity by mouse animal models. The main effective components of soybean were pinitol and inositol that identification of bioactive compounds inhibited cell motility. Also anti cancer marker such as ICAM-1, CD44, MMP17, MMP14 and ARF2 decreased expression in B16 cells. Inositol inhibited MMP14 and VEGF-A expression. Anti-inflammatory effects associated with atopy in animal models showed, in atopic derived model of the ear thickening (thickness) and reduces the movement of immune cells to sites of inflammation were reduced.
○ In a first experiment, 12 soybean varieties (Dae-mang, Dae-won, Dae-poong, Seok-ryang-put, Seon-yu, Shin-gi, Aga, IL-pum-geomjung, Chung-ja, Keun-ol, Tae-kwang, Poong-san herb hefor the extraction of -jaithin was the best solvent extraction De-gumm Tae-nd hydrate extraction using supercritical extraction methods were extracted due to lecithin.
○ Results, De-gumming and hydration through a method to extract and produce large amounts of lecithin was best suitable. Using solvent extraction yield but high due to the use of organic solvents cause environmental pollution problems are likely to have been determined to be unsuitable for supercritical extraction of lecithin, you get the quality of lecithin, but due to the extraction solvent extraction or hydration separation unit is very expensive compared to the bulk separation of systems was considered unsuitable.
○ Soybean isoflavone present the main ingredient of Lecithin and Genistein a prototype was developed Essence Cream. Genistein also has antioxidant properties that reduce UV damage to DNA through the action's primary effect of massage cream took the concept and Lecithin was extracted from the beans as a natural emulsifier for cosmetic formulations designed Paid (Oil Phase) in the main material composition used.
○ Emulsion stability of this chemical emulsifiers in addition to promoting the use of natural materials was compared to the benefits derived from soybean and two kinds of graft material in the manufacture of cosmetics Emulsion forms the essence of the prototype has been developed.
○ Genistein and lecithin from soybeans Isoflavone to present the main ingredient of a prototype essence cream was developed. Genistein also has antioxidant properties that reduce UV damage to DNA through the action's primary effect of massage cream took the concept and Lecithin was extracted from the beans as a natural emulsifier for cosmetic formulations designed Oil Phase on the composition as the main material used. Emulsion stability of this chemical emulsifiers in addition to promoting the use of natural materials was compared to the benefits derived from this are two kinds of beans in the manufacture of cosmetic ingredients by combining the essence of Emulsion forms a prototype was developed.
○ In the 2nd research, isolated from the previous experiment 12 cultivar of crude soybean oil Lecithin content and Phosphatidylcholine (PPC) content of Colorimetric determination assay were determined. Functional compounds, lecithin, and PPC at a large separation of these compounds to evaluate the varieties were the highest and Results, PPC Seon-Yu the lecithin content in the most representative was selected as the best cultivar Aga soybeans with higher than average respectively. PPC Lecithin extracted from the fat by enzymes extracted from soybean Natural Cosmetics industry by integrating concept Slimming Gel (tentative title) in the form of cosmetics and get a great response when the development is considered.
○ Soybean production technology in the past on the basis of experiments with very high isoflavone content of Aga-3 as a main manufacturer of soy milk and fermented soy milk with lactic acid bacteria inoculated manufacture of prototypes and functional analysis was performed accordingly. The last time developed a prototype fermented soymilk was vitamin D, folic acid, GABA and other products that do not have to contain useful physiologically active substances were found. Fermentation strains in the immune enhancement effect of Leuconostoc kimchii and improve bowel function and represents the antioxidant activity of Leuconostoc citreum and cholesterol lowering efficacy in cardiovascular disorders such as Lactobacillus plantarum was used in sensory evaluation because of severe superior functionality compared to the smell of Many people were reluctant, but overall progress is promising possibilities were evaluated.
○ In the 3rd research, This is the main material for manufacturing soy milk experiment Aga-3 based on the manufacture of fermented milk have not been functional for being tough as compared to fermentation and 5 cultivar except for Aga soybean varieties (Tae-kwang, Seo-mok-tae, Baek-tae, Sok-cheong, Hwa-tae) were also analyzed. Among the several cultivar of the functional and sensory evaluation by the panel preferred the soy drink cultivar and comprehensive development of superior varieties are meant to choose. First, a functional effect on whether the comparison is as follows.
Affects the fermentation of soybean oil that was about the first to investigate the effect of antioxidant effect in the analysis of a product that dominates the overall result was to inhibit the absorption of nutrients Phytate content analysis and high blood pressure causing increased blood pressure ACE inhibition is also fermented soymilk is higher than non fermented the activity showed the results.
○ Aga soybeans compared with other varieties and analysis were as follows, Analysis of antioxidant ABTS radical scavenging activity and DPPH radical scavenging activity showed the Seo-mok was best results. In the SOD-like activity of the Aga was the most excellent results. Analysis of functional materials, the Total Polyphenol and Total Flavonoid, Phytate content, ACE Inhibition, GABA content, determination of free amino acids, such as antidiabetic in experiments with both Aga most outstanding feature was found. Aga-3 in sensory evaluation of all varieties were superior rating also increases the useful functionality and panel manufacturing soy beverages will very likely emerge as a material, as well as increased domestic consumption of soybeans and is expected to contribute to farm income.
○ Effects of Lunasin enriched source(LES), fractionated from soybean extract, on the recovering against inflammation in the skin of mice and on the expression of the pro-inflammatory factors, Cox-2 and iNOS, were investigated after treatment LES in inflammation-introduced mice skin with DNCB in vivo, by scanning electron microscopy and immunoblot analysis. Also anti-oxidation effect and protecting effect against UV were detected with cultured celll and mice. Treatment with LES showed that recovering effect from tissues suffered skin inflammation and downregulatory role in expression of iNOS and Cox-2 genes. Moreover lunasin peptide was detected in nuceous after treatment with LES on the skin by confocal microscopy analysis. Taken together, LES from soybean extract is effective anti-inflammation agent and is able to use for cosmetic and/or therapeutic substances.
○ Genistein decreased cancer cell viability in a dose-dependent manner, whereas daidzein did not show dramatic cytotoxic effects. The results of RT-PCR and real-time PCR indicate that a combination of genistein and daidzein can synergistically induce NAG-1 expression in HCT116 cells.
This result implies that NAG-1 induction is highly associated with synergistic cytotoxic effect induced by a combination treatment of genistein and daidzein. The extraction yield of phytic acid was 0.32% when extracted with citric acid solution. The extract showed a good antioxidant activity as well as antimicrobial activity against S. aureus, P. aeruginosa , B. subtilis and E. coli. When the 3T3-L1 preadipocyte cells were treated with soy extract, their differentiation into adipocytes were slightly inhibited and the lipid accumulation in the cell was also diminished. In the animal trial for anti obesity experiment, animal group fed with fermented soybean showed a significant decrease in weight gain rate and cholesterol level in blood.
○ The superior strains generating fibrinolytic enzymes were selected and the optimum fermentation condition by the use of Agabean were established. The extract from the fermentation of Agabean exhibited a variety of physiological activities including anti-oxidation, anti-diabetes and anticancer. In addition, its preference assessment through the organic examination gave the high valuation among the bean products .
○ Blood glucose-improving effect, blood glucose regulation, and fatty liver-improving effect by the extract of Agabean and fermented Agabean were higher than those by the extract of soybean. The extract of the fermented Agabean showed the high inflammation and anticancer activity. In addition, the effective constituent of the bean showed the high activity in the suppression of tumor metastasis and anticancer.
○ The substances showing the anti-obesity activity were purified from the bean and analyzed for the activity suppressing obesity. The anti-obesity beverage was developed by the use of the substances, and the beverage was superior to other products in anti-obesity.
○ Pedigree of the high quality black bean was performed and anthocyanin from the bean shell was analyzed to the physiological activity related to the inflammatory stress. As a result, the pigment had a crucial effect on arteriosclerosis, diabetes, anti-oxidation and aging suppression.
○ Manufacturing process of soybean protein was developed. Antihypertensive peptide was manufactured from soybean protein using protease. Soybean protein isolate was hydrolysed by Alcalase 2.4 L, ACE inhibitory activity of soybean protein isolate hydrolysates were investigated. IC50 value of ACE inhibitory activity of soybean protein isolate hydrolysate was 79.94 ㎍ /mL. Antihypertensive products(Tablet, capsule, pill) was manufactured using soybean peptide.
○ In Function analysis of total phenol content of OD 765ml, the higher concentration of proteolytic enzyme is, the phenol rate in whole-soy oil is higher. In whole-soy oil made by processing proteolytic enzyme of 0.5% degree, phenol was 750ug/ml. DDPH free radical absorbance capacity was slightly increased in processing proteolytic enzyme. In analysis of blood circulating function, glucose in blood, GOT, neutral fat, and cholesterol have been significantly decreased.
○ Whole-soy oil production : Preparing and designing of sample products by OEM is completed.

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 요약문 ... 4
• SUMMARY ... 22
• CONTENTS ... 44
• 목 차 ... 46
• 제 1 장 연구개발과제의 개요 ... 48
• 1. 연구개발대상 기술의 배경 및 연구개발의 필요성 ... 48
• 2. 연구 목표 ... 54
• 제 2 장 국내외 기술개발 현황 ... 57
• 제 3 장 연구개발수행 내용 및 결과 ... 62
• 제 1 절 내도복 다수성 고 기능성(isoflavone) 콩 품종육성과 기능성 콩 분말제품 개발 및 산업화 ... 62
• (시험 1) 내도복 다수성 고 기능성 콩 품종육성(황영현) ... 69
• (시험 2) 아가콩을 이용한 기능성 제품 산업화(위탁-정연신:소이벤처) ... 85
• 제 2 절 양질 지방산 함유 콩 품종개발 ... 129
• 1. 재료 및 방법 ... 129
• 2. 연구결과 및 고찰 ... 136
• 제 3 절 신품종의 친환경 재배시스템 개발 ... 179
• 1. 신품종의 친환경 재배시스템 개발 ... 179
• 2. 품종별 친환경 병충해방제 기술개발(위탁) ... 196
• 제 4 절 신품종 육종공의 의학적 약리효능구명 ... 224
• 1. 신품종 육종콩의 의학적 약리효능 구명 ... 224
• 2. 신품종 육종콩의 기능성 탐색 및 기능성분 구명 ... 238
• 3. 육성품종 콩을 이용한 기능성 청국장의 개발 ... 250
• 4. 콩의 주요 성분을 이용한 산업화 연구 ... 272
• 제 5 절 기능성 물질의 대량 분리 생산 및 산업화 ... 289
• 1. 콩의 레시틴 분리 ... 289
• 2. 콩 유래의 이소플라본과 레시틴이 함유된 에센스시제품 개발 ... 305
• 3. 발효 두유 시제품 개발 ... 318
• 4. 발효 여부에 따른 두유의 기능성 비교․분석 ... 322
• 5. 콩 품종간 두유의 기능성 비교․분석 ... 332
• 제 6 절 콩 기능성 물질을 이용한 건강기능식품 및 미용제품개발 ... 356
• 1. 콩 저분자 단백질을 활용한 피부개선 효능 분석 및 제품 개발 ... 356
• 2. 콩 기능성 물질의 효능 검정 및 건강식품 개발 ... 385
• 제 7 절 생활습관성 질환개선제품 개발 ... 424
• 1. 생활습관성 질환 개선제품 개발 ... 424
• 2. 신품종 콩을 이용한 고혈압 개선 제품개발 ... 527
• 제 8 절 대량수요를 창출하는 제품개발 ... 563
• 1. 실험방법 ... 563
• 2. 연구결과 ... 568
• 제 4 장 목표달성도 및 관련분야에의 기여도 ... 587
• 제 5 장 연구개발 성과 및 성과활용 계획 ... 590
• 제 6 장 연구개발과정에서 수집한 해외과학기술정보 ... 594
• 제 7 장 참고문헌 ... 596
• <부록>. 한국, 중국, 미국 및 일본 콩 유전자원의 지방 및 지방산 함량 ... 635
• 끝페이지 ... 647