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Kafe 바로가기주관연구기관 | 경상대학교 GyeongSang National University |
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보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 | 한국어 |
발행년월 | 2012-02 |
과제시작연도 | 2010 |
주관부처 | 농림축산식품부 Ministry of Agriculture, Food and Rural Affairs(MAFRA) |
등록번호 | TRKO201400026337 |
과제고유번호 | 1545002043 |
사업명 | 농림기술개발 |
DB 구축일자 | 2014-11-10 |
DOI | https://doi.org/10.23000/TRKO201400026337 |
○ 연구결과
1)돼지 혈청에 대한 사료 첨가제의 영향을 조사하기 위해 혈청에 대한 proteome:Sarcolemmal membrane associated protein, cholesterol 대사 관련 지단백질인 Apolipoprotein A-IV, unnamed protein product, apolipoprotein 전구 물질로 작용하는 단백질인 apolipoprotein A-I preproprotein 등이 Up-regulation 되었으며, hypothetical protein isoform 4 는 Down-regul
○ 연구결과
1)돼지 혈청에 대한 사료 첨가제의 영향을 조사하기 위해 혈청에 대한 proteome:Sarcolemmal membrane associated protein, cholesterol 대사 관련 지단백질인 Apolipoprotein A-IV, unnamed protein product, apolipoprotein 전구 물질로 작용하는 단백질인 apolipoprotein A-I preproprotein 등이 Up-regulation 되었으며, hypothetical protein isoform 4 는 Down-regulation
2)대조군에 비하여 조지방의 감소(6.63±0.48▶4.01±0.19),토탈 cholesterol의감소(62.29±0.28▶52.18±0.27) , 사료요구율(3.28▶2.57)의 감소, 체중(98.1▶100.12)의 증가
3)IgY가 함유된 사료첨가제가 돼지 체내 안전성 평가:Alkaline phosphate, SGOT, SGPT, Amylase, glucose, bilir ubin, LDH,PK등이 정상범위
4)사료첨가제를 급여한 군 (1%)이 포화지방산의 감소,불포화지방산의 증가
5)천연소재 약용자원과 IgY의 사료 급여를 통한 돼지 소화기성 질병에 대한 항질병효과가 규명 됨
6)천연소재 약용자원과 IgY의 사료 급여를 통한 돼지 정상 세균총에 대한 안전성이 확보됨
7)천연소재 약용자원과 IgY의 대식세포 활성 및 사료 급여를 통한 돼지 체내 면역 활성이 확인됨었으며, 6개월 후에는 5배 가량 적은 것으로 나타났음
8)사료첨가제 사용시 MSY(15.2▶21)증가등으로 소득증대 향상이 예측됨
4 Results of Research
The effect of the natural herb additive on the serum GOT/GPT levels was examined by monitoring the pigs daily. On the 1st day, the GOT/GPT level was 67:4 3:1=69:65 7:56 IU/L (control group), 56:87 10:09=69:76 4:12 IU/L (0.5% additive group) and 102:81 7:13=86:46 10:28 IU/L (
4 Results of Research
The effect of the natural herb additive on the serum GOT/GPT levels was examined by monitoring the pigs daily. On the 1st day, the GOT/GPT level was 67:4 3:1=69:65 7:56 IU/L (control group), 56:87 10:09=69:76 4:12 IU/L (0.5% additive group) and 102:81 7:13=86:46 10:28 IU/L (1% additive group). After eight weeks, the GOT/GPT level was 73:07 7:08=80:29 1:37 IU/L (control group), 46:29 4:28=81:44 3:36 IU/L (0.5% additive group) and 42:25 4:63=76:66 1:18 IU/L (1% additive group).
On the 1st day, the body weight gain showed a similar course in the control and 0.5% and 1% additive groups. After eight weeks, the body weight gain was 39:9 1:25 kg (control group), 45:1 1:10 kg (0.5% additive group) and 42:5 1:27 kg (1% additive group). No impairment in the animal health status was observed.The proteins from the spleens of the control and eight weeks additive supplemented pigs (0.1%) were extracted and resolved by 2DE using pH 3–-10 IPG strips loaded with 100 g of the total proteins. The molecular weights of the spots ranged from 7 to 175 kDa.
The differences in the spot intensity were identified as quantitative changes. A total of 31 differentially expressed protein spots were identified (more than two-fold was considered significant), and finally 19 were detected by MALDI-TOF/MS. Among the 19 protein spots, 7 and 12 proteins were significantly up-regulated and down-regulated, respectively.Thirty seven percent of the proteins identified were involved in the cellular components and cells (GO: 0005575 and GO: 0005623 respectively). Moreover, there were 17% intracellular proteins (GO: 0005623), 13% cytoplasm (GO: 0005634) and organelle involved proteins (GO: 0005737). For the biological process, the strongest associations were with the metabolic process (26%) (GO: 0008152). Another 22% associations were with the biological process (GO: 0008150), whereas 8% were associated with transport (GO: 0006810) and 7% with the carbohydrate metabolic process and the generation of precursor metabolites and energy (GO: 0005975 and GO: 0006091).From this study, we found that the antibacterial effects of Saururus Chinensis and Scutellariae Radix extract against E.coli and Salmonella typhimurium in vitro and IgY which is producted by E. coli and Salmonella typhimurium Ag immunization showed the inhibitory effect bacterial internalization and intracellular replication within phagocytes. In vivo test, swine group which is fed with natural herb, IgY antibody and bio-balance materials composing feed showed a lower virulence expression than those of the fed with normal feed.
Moreovere, swine group which is fed with natural herb, IgY antibody and bio-balance materials composing feed did not affect the normal flora in the intestine and showed increasing WBC in whole blood comparing to untreated control groups. Conclusionly, natural herb and IgY showed an antibacterial effect against pathogenic bacterial but not normal intestinal bacteria, and natural herb, IgY antibody and bio-balance materials composing feed showed immune activation and diarrheal disease controlling in swine.
From this study, we found that the immunospecific IgY against E. coli and Salmonella typhimurium and IgY treatment inhibited the bacterial internalization and adherence on epitherial cells. Moreovere, swine group which is fed with natural herb, IgY antibody and bio-balance materials composing feed showed a higher white blood cells in whole blood than those of control groups, suggesting that natural herb, IgY antibody and bio-balance materials composing feed may increase immunostimulatory effect in swine.
In addition, we found that quercetin, isolated from O. humifusa Raf, showed potent antioxidant and anti-inflammatory activities. Furthermore, these data suggest that quercetin has potent antioxidant and anti-inflammatory effect in vitro. Data of reporter gene luciferase assay suggest that anti-inflammatory effects might be mediated in the levels of transcription. Platelet aggregation was induced either by ADP, a potent agonist to platelet G protein-coupled P2Y receptor, by collagen, a potent ligand that activates platelet integrin α2β1 and glycoprotein VI, or thrombin, a platelet protease-activated receptors subtype I and IV. This preliminary result suggests that O.humifusa may be taken as a candidate lead natural compound to be considered in the search for natural products with beneficial effects on aberrant platelet activation mediated cardiovascular disorders. Anti-platelet activities of CE6 were studied using rat platelets. We examined the effect of CE6 on intracellular Ca2+ mobilization using fura-2/AM, and determined ATP release and cyclic nucleotides production. These results suggested that cAMP elevating effect of CE6 might be due to the PDE inhibiting activity rather than AC activation.
In conclusion, results of the experiments suggest that Natural Herb Additive Containing Immunoglobulin Yolk sac (IgY) were effective in improving in pig on growth performance, physico-chemical properties and prevention of various digestive diseases and may increase economic return.
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