보고서 정보
주관연구기관 |
바이오컬쳐(주) |
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 |
한국어
|
발행년월 | 2012-04 |
과제시작연도 |
2011 |
주관부처 |
농림축산식품부 Ministry of Agriculture, Food and Rural Affairs(MAFRA) |
등록번호 |
TRKO201400026453 |
과제고유번호 |
1545002629 |
사업명 |
첨단생산기술개발 |
DB 구축일자 |
2014-11-14
|
DOI |
https://doi.org/10.23000/TRKO201400026453 |
초록
▼
■ 연구결과
-생체 삽입형 바이오센서 설계 :4종(링타입,T자형 및 코일형)
-무구속 장착(생체 삽입형)을 위한 바이오센서 내장 삽입 장치 개발
-바이오센서(Temperature,pH)내장 및 센서 인터페이스 회로 설계
-실시간 모니터링 시스템 모듈(ASIC)설계
-생체 내장 바이오센서의 실시간 무선 전송 시스템 확립
-시제품 제작 및 멀티 바이오센서의 생체 적용하여 센싱 data분석
-생체 삽입형 바이오 온도 및 pH 센싱에 의한 번식관리 지표 설정 및 알림 기능 확립
*발정,수정적기(임
■ 연구결과
-생체 삽입형 바이오센서 설계 :4종(링타입,T자형 및 코일형)
-무구속 장착(생체 삽입형)을 위한 바이오센서 내장 삽입 장치 개발
-바이오센서(Temperature,pH)내장 및 센서 인터페이스 회로 설계
-실시간 모니터링 시스템 모듈(ASIC)설계
-생체 내장 바이오센서의 실시간 무선 전송 시스템 확립
-시제품 제작 및 멀티 바이오센서의 생체 적용하여 센싱 data분석
-생체 삽입형 바이오 온도 및 pH 센싱에 의한 번식관리 지표 설정 및 알림 기능 확립
*발정,수정적기(임신진단)및 분만의 시기의 자동 통보(알림)장치(기구)개발
-완제품의 상품화 추진(전시회 또는 박람회 참관,홍보)
Abstract
▼
The livestock industry in Korea urgently needs the improvement of productivity and reliability through better working conditions, better price competitiveness and scientific breeding. This study attempts to develop ubiquitous breeding system for farms to monitor index at real-time basis and control
The livestock industry in Korea urgently needs the improvement of productivity and reliability through better working conditions, better price competitiveness and scientific breeding. This study attempts to develop ubiquitous breeding system for farms to monitor index at real-time basis and control and measure it using bio-sensing. As convergence technology with IT and BT under USN(Ubiquitous Sensor Network) circumstance, it may contribute to produce highly reliable information for better productivity and quality improvement.
It is global trend to transfer livestock industry to underdeveloped countries due to environmental pollution or to transfer full-time farming or commercialized farming which requires more advanced control due to the increase of number of livestock, and for environmental condition improvement. Furthermore, as there is a risk of huge economic loss and social problem by infectious diseases, real-time health and disease monitoring is urgent to prevent endemic disease. Increase of productivity through effective breeding including sound nurturing, estrous detection via bio-signal, breeding time control and pregnant and delivery are utmost important. A system development to protect workers from any unpredictable acts by the livestock and to make a ubiquitous control outside the cattle shed is necessary. Also a technology to supply necessary information for livestock industry policy making and research project such as livestock product record system and gene resource preservation, protection and productivity. BT is one of national core projects with high demand of IT and BT convergence. Furthermore, animal bio-injection biosensor is a global trend of development. Therefore, it is very time to study interdisciplinary study with biology, livestock, veterinary science, biosensor and resources and to preoccupy a global market with original technology.
This study explored biosensor for bio information collection of livestock (pig), applied biosensor after designing and manufacturing ASCI for real-time wireless transfer and achieved real-time monitoring.
The following results are drawn as reproduction control index.
1. Temperature change during the estrous time of sows was 36.9±0.4℃ right after ablactation (Day -2), which reached the maximum on the Day 2 with 37.8±0.2℃ after two days of estrous start (Average 37.6±0.2℃). Then, it continued to decrease down to the same level before estrous start.
This result indicates the estrous start of sows is the point of 0.7℃ increase of temperature and the optimal temperature of fertilization is the highest temperature with 37.6~38.0℃ range.
2. Temperature change during the estrous time of gilts was 36.9±0.4℃ right after removing Ring-type (Day -2), which reached the maximum on the Day 2 with 37.8±0.2℃ after two days of estrous start (37.6±0.2℃). Then, it continued to decrease down to the same level before estrous start.
This result indicates the estrous start of gilts is the point of 0.7℃ increase of temperature and the optimal temperature of fertilization is 37.7~38.1℃ range.
3. PH change during the estrous time of sows was 6.77 right after ablactation (Day -2), which reached the maximum on the Day 2 and Day 3 with 7.4 after two days of estrous start (Average 7.1). Then, pH continued to decrease down to the same level before estrous start. As pH sensor insert was difficult to gilts, the size was needed to be shortened. Thus it did not take as existing pH sensor could not be inserted. Meanwhile, pregnant diagnosis index failed due to high stress of biosensor insertion, artificial fertilization and re-insertion. However, data oscillation device had MSP430(MCU) built-in system with Tiny-OS, and it determined regular transfer condition and time by programming with NesC. Data receiving device was programmed with NesC on Tiny-OS and interlocked to database which enabled SMS sending using JAVA program. The SMS sending was made possible by registering mobile number in LG-Dacom.
To develop pregnant diagnosis index, biosensor (temperature) was inserted inside the vagina before starting estrous. If estrous start and optimal time of fertilization are notified, it removed the sensor (remove it and re-insert after fertilization) and attempted fertilization. An attempt to develop pregnant diagnosis index was carried out based on an hypothesis that if the process was properly done, it developed to pregnant and the womb temperature would be kept rather higher by activating the womb (See fig. 53). But it failed to develop the index as the stress of test stock was too high due to biosensor insertion, artificial fertilization and re-insertion.
The principle of pregnant detection device by temperature is that if livestock (pig) starts delivery, the allantois is destroyed to release lumbar segment, which is followed by the breakdown of the amnion and release of amniotic fluid. Here, biosensor (tempo-sensor: 3 types) in the vagina is released together with lumbar segment and amniotic fluid. If tempo-sensor exists in the vagina, it keeps 37.
5℃, and it is decreased down to the room temperature (20~25℃) when it is released. At this time, if the temperature becomes lower than 30℃, the tempo-sensor causes wireless LAN module interface to notify it to mobile or internet of the manager on the deliver condition. Such delivery detection principle is designed by recognizing the temperature differences between body temperature and external temperature. The scope of temperature difference is highly related to the temperature of cattle shed. Thus, it does not affect much during winter, but the temperature must be set rather higher than that in cattle shed during the high temperature period in summer. For delivery detection by tempo-sensor, wireless LAN interface operation is essential and low power battery is to be used.
The following conclusions are drawn from the livestock test result by trial goods.
1. More complementation of communication distance and power device operating time (lifespan) of body insertion biosensor (temperature and pH) is required.
2. The reduction of body insertion pH sensor must be needed, pH measurement per use (reuse) is required and it was difficult to maintain reliability.
3. Insertion biosensor for real-time monitoring caused hygiene inconveniences and stress to target livestock.
4. Large size stock is more advantageous in insertion treatment than medium or small size livestock for applying real-time breeding index and health control system by biosensor.
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