초록 ▼

Ⅴ. 연구개발결과
제 1 세부과제
○ 양배추 및 다양한 근연 배추속 식물들의 유전체 정보 수집 및 활용체계 구축
○ 국내 육성계통 간에 약 800개 이상의 다형성 마커 개발
○ 국내 elite 육성 계통인 C1184와 C1234 간 354개 마커로 구성된 유전지도 작성
○ 개발된 마커를 이용한 91개의 전세계 상업적 양배추 품종의 유전적 다양성 분석
○ 양배추에서 발현된 여러 유전자들의 정보가 포함된 EST 정보 생산
○ 4계통에 대한 WGS 정보 생산 및 양질의 SNP 지역을 발굴할 수 있는

Ⅴ. 연구개발결과
제 1 세부과제
○ 양배추 및 다양한 근연 배추속 식물들의 유전체 정보 수집 및 활용체계 구축
○ 국내 육성계통 간에 약 800개 이상의 다형성 마커 개발
○ 국내 elite 육성 계통인 C1184와 C1234 간 354개 마커로 구성된 유전지도 작성
○ 개발된 마커를 이용한 91개의 전세계 상업적 양배추 품종의 유전적 다양성 분석
○ 양배추에서 발현된 여러 유전자들의 정보가 포함된 EST 정보 생산
○ 4계통에 대한 WGS 정보 생산 및 양질의 SNP 지역을 발굴할 수 있는 pipepline 구축
○ 흑부병 저항성 후보 QTL 지역 동정
○ 양배추를 포함한 여러 Brassica species내 여러 MITE family들의 동정 및 이를 이용한 마커 개발

제 1 협동과제
○ 수출용 고품질 흑부병 저항성 품종 육성
(총 11 품종 개발 - 품종보호출원 5 품종, 생산판매신고 6 품종)
- 중국용 원형 극조생계 품종 육성
1) 패스트볼(Fast Ball) - 품종보호출원번호 : 출원 2012-89
2) 에이스볼(Ace Ball) - 품종보호출원번호 : 출원 2014-106
- 인도용 원형 조생계 저항성 품종 육성
3) 포르테 9 (Forte 9) - 품종보호출원번호 : 출원 2014-107
4) 비너스 11 (Venus 11) - 생산판매신고 필증번호 : 02-0003-2014-7
- 인도, 중국용 원형 조생계, 중생계 흑부병 저항성 품종 육성
5) 스마트(Smart) - 생산판매신고 필증번호 : 02-0003-2009-10
6) 조은에이스(Joeun Ace) - 품종보호출원번호 : 출원 2012-90
7) 엘로라 (Ellora) - 생산판매신고 필증번호 : 02-0003-2014-7
- 동남아시아 및 중국용 편형계 흑부병 저항성 품종 육성
8) 오션 99 (Ocean 99) 생산판매신고 필증번호 : 02-0003-2014-5
9) 트로픽 에이스(Tropic Ace) - 생산판매신고 필증번호 : 02-0003-2012-1
10) 그린스페이스(Green Space) - 품종보호출원번호 : 출원 2012-88
- 자색 양배추 품종 육성
11) 자색양배추 베로나(VERONA) - 생산판매신고 필증번호 : 02-0003-2014-2
- 고순도의 종자 생산과 해외 채종시 원종 유출 방지를 위한 세포질 웅성불임성을 도입한 채종 체계 구축
○ 흑부병(검은썩음병) 및 뿌리혹병 저항성 검정 및 검정 방법 확립
- 국립 화학연구원에 의뢰하여 국내외 수집 균주를 이용하여 효율적이고 정확한 병리 검정 방법 확립 및 저항성 검정
- 흑부병 및 뿌리혹병 저항성 소재의 저항성 유전 인자를 확인하고 유전 양식 연구를 통해 저항성 품종 개발을 위한 기초 연구

Abstract ▼

In response to our dependent on foreign market demand of Brassica oleracea species the development cabbage seed with black rot resistance as well as high quality, high value, and market preference products that causes the problem during growing and also favorable within these region is necessary to

In response to our dependent on foreign market demand of Brassica oleracea species the development cabbage seed with black rot resistance as well as high quality, high value, and market preference products that causes the problem during growing and also favorable within these region is necessary to expand the business as seed exporting market. In addition, development of resistance marker for black rot and club root while constructing high resolution molecular genetic map to create molecular breeding system for cabbage’s breeding efficiency enhancement would in the future contribute our Korean cabbage varieties towards global competitiveness and export market expansion in overseas markets.
The application and the genetic information of B.oleracea and it’s closely related Brassica species, was established by sharing information from International Brassica rapa genome projects and joint projects. It also allowed to developed varieties of markers on domestic elite breeding line using the markers obtained from genetic map and the EST and WGS(Whole Genome Sequencing) information using NGS(Next Generation Sequencing) technology of B. oleracea and B. napus. Utilizing the markers of domestic elite breeding line, molecular genetic map formed by 354 markers related to black rot resistance was created. Moreover, black rot pathology test targeting F₃ individual was tested about 3 times repeatedly. By linking both result of vaccination of black rot and the genetic result map, the candidate of black rot resistance QTL regions were identified.
Within combination of club root related, about 78 of F₂ individuals have been genotyped around 15,000 SNP regions following GBS(Genotyping by Sequencing) method.
Within 35 developed markers the genotyping of F₂ individuals have been completed and the high-density genetic map using F₂ genotyping results is still under process. The result of club root disease pathology test, for race 9 strain by repeating 2 times each while race 2 and 5 strains has each gave clean result just by performing only one time. As the genetic map completes by linking result from pathology test we could candidate the club root resistance QTL regions by each race strains.
Among many markers developed during the course of research, 184 SSR markers were used for genetic diversity analysis with worldwide 91 commercial varieties of B. oleracea, and we could obtain 16 cultivar specific markers. As the result of this experiment, various consume species could each be identified and developed for species varieties of distinctness, uniformity, stability to utilize as a useful test. We also performed research on MITE(Miniature Inverted-repeat Transposable Element) family’s identification and characterization of brassica species including cabbage. Utilizing it could further developed 36 inter-species classification polymorphic marker and 90 intra-species classification polymorphic marker.
Total 11 commercial varieties were developed for China, India and South East Asia markets. Fast Ball and Ace Ball were developed for China market and they have round head shape and green head color and extra early maturity and stronger cracking tolerance.
Forte 9 was developed for India market and its maturity is 2∼3 days earlier, more compact and greener than check varieties. Venus 11 is 5∼6 days earlier, its plant frame is more compact, greener and stronger cracking tolerance than check varieties. Smart was developed for heat tolerant, round and mid-early maturity group market in India, China and S.E. Asia. It has more compact, more stable core, stronger cracking tolerance and black rot resistance than check varieties. Joeun Ace was developed for China, India and Pakistan market. It has about one week earlier, more green color and stable head shape than check varieties and it is suitable for high density transplanting and it has more green head color, stronger transportation ability, cracking tolerance and better taste. Joeun Ace showed stronger black rot resistance than check varieties in India local trials and in vivo test.
Ellora was developed to replace 'Green Express' from Sakata, 'T-621' from Takii, Zennith and Millennium 111 from Seminis in India market. It has one week earlier than check varieties and its heat tolerance, black rot resistance and cracking tolerance level was increased up to heat tolerant type varieties. Ocean 99 was developed to replace ‘KK-Cross' from Takii and ‘KS-Cross' from Syngenta in South China market, West Bengal market in India and S.E. Asia market. Ocean 99 is 4∼5 days earlier and it has stronger heading ability under hot temperature condition, stronger cracking tolerance and stronger black resistance than check varieties. Tropic Ace was developed to replace 'KK-Cross' and Grand KK from Takii. Its head shape is more stable and stronger heading ability under hot temperature condition. Green Space was developed to replace 'New Star Cross(NSX)' from Tokita in Vietnam market. It has earlier maturity, heavier head weight, stronger cracking tolerance and black rot resistance. Verona was developed for purple cabbage market and it has 75 day maturity, round head shape and excellent internal purple color and good results in China, India and Europe market.
The segregation ratio in F₂ population derived from the cross between black rot resistant line and susceptible line(C1184 x C1234) was R(resistant) : MR(intermediate) : S(susceptible) = 63 : 45 : 34( 1.9 : 1.3 : 1) and R+MR(resistant + intermediate) : S(susceptible) = 3.2 : 1. The black rot resistance of F₃ population was tested during 2013 and 2014 and it was used for development of marker in Seoul National University.
The segregation ratio in F₂ population derived from the cross between clubroot resistant line and susceptible line (C1220 x C1176) was R:MR:S=62:62:68(1:1:1) and the ratio of C1176 x C1220 F₂ population was 159:32:3 and the ration of C1220 x C1126 F₂ population was R:MR:S=147:30:21 and the ration of C1126 x C1220 F₂ population was R:MR:S=67:77:53. The clubroot resistance of F₃ population was tested during 2013 and 2014 and it was used for development of marker in Seoul National University.

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 요 약 문 ... 3
• SUMMARY ... 7
• CONTENTS ... 9
• 목차 ... 10
• 제 1 장 프로젝트의 개요 및 성과목표 ... 11
• 1. 연구개발의 필요성 ... 11
• 2. 연구개발의 최종 목표 ... 11
• 3. 연구성과 목표 대비 실적 ... 11
• 제 2 장 국내외 기술개발 현황 ... 12
• 1. 국내외 관련분야 기술개발 현황 ... 12
• 2. 연구 결과가 국내외 기술 개발 현황에서 차지하는 위치 ... 13
• 제 3 장 연구개발수행 내용 및 결과 ... 14
• 1절 양배추 분자유전지도 작성 및 흑부병과 뿌리혹병 저항성 연관 마커 개발 (제1 세부과제) ... 14
• 2절 수출용 고품질 흑부병 저항성 양배추 품종육성 (제1 협동과제) ... 64
• 제 4 장 목표달성도 및 관련분야에의 기여도 ... 99
• 제 5 장 연구개발 성과 및 성과활용 계획 ... 101
• 1. 연구성과 목표 ... 101
• 2. 연구 성과 활용 계획 ... 107
• 제 6 장 연구개발과정에서 수집한 해외과학기술 정보 ... 109
• 1. 양배추 표준유전체 염기서열 확보 ... 109
• 제 7 장 참고문헌 ... 110
• 끝페이지 ... 112