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Kafe 바로가기주관연구기관 | 서울대학교 Seoul National University |
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연구책임자 | 박충모 |
참여연구자 | 류재용 , 이상민 , 윤주 , 정은영 , 박미정 , 이재형 , 그외 다수 |
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 | 한국어 |
발행년월 | 2015-02 |
과제시작연도 | 2014 |
주관부처 | 농촌진흥청 |
사업 관리 기관 | 농촌진흥청 Rural Development Administration |
등록번호 | TRKO201500010197 |
과제고유번호 | 1395035356 |
DB 구축일자 | 2015-07-11 |
Ⅳ. 연구개발결과
- FCA-SE module과 circadian clock 상호관계 연구/ brachypodium과 벼에서의 artificial siPEP 작용연구
- 저온 내성 관련 유전자 도입 형질전환 오이 식물체 제작/ 형질전환 식물체의 분자생물학적 특성 및 생리/생화학적 특성 분석
- SRD2 유전자 및 단백질 발현 위치 확인/ SRD1, SRD3 당근 형질전환체 제작
- RabG3bCA 형질전환 포플러의 포장시험 진행/ 포장에서 RabG3bCA 형질전환 포플러의 생장 관찰 및 측정
Plants have evolved an array of defense mechanisms that cope with abrupt changes in environmental conditions, among which plant responses to cold temperatures have been expensively studied. Under cold temperature conditions, plants adjust their physiology, such as induction of a series of cold-respo
Plants have evolved an array of defense mechanisms that cope with abrupt changes in environmental conditions, among which plant responses to cold temperatures have been expensively studied. Under cold temperature conditions, plants adjust their physiology, such as induction of a series of cold-responsive genes and production of osmoprotectants and anti-freeze proteins, and morphology through differential organogenesis. The best characterized cold signaling scheme is the ICE-CBF-COR module, in which the master transcription factor ICE proteins induce the expression of the CBF transcription factors that regulate the downstream COR genes.
In this work, we further extended the repertoire of the signaling components functioning through the ICE-CBF-COR module. Of particular interests is the close linkage between the circadian clock and cold response. One example is the cold-responsive alternative splicing of the CCA1 gene that is a critical component of the plant circadian clock. Upon exposure to cold temperatures, CCA1 alternative splicing is induced, producing two protein splice variants. The small form itself does not have transcriptional regulatory activity. However, it forms a nonfunctional heterodimers with the larger CCA1 forms, which are excluded from the promoters of the target genes. Interestingly, the induction of CCA1 alternative splicing interferes with the clock function. These observations indicat that whereas under normal conditions, the energy-consuming clock function is required to maintain normal plant growth and development, the clock function is suppressed to redistribute the metabolic energy for the induction of cold responses. We propose that the intimate linkage between the clock and cold response has been evolved to provide an adaptive strategy under fluctuating temperature conditions.
Our findings will provide a novel way of improving plant resistance to cold temperatures by including the clock component into the genetic engineering studies on plant cold response. One good aspect of the colck-based engineering of cold response is that plants that have altered clock function do not exhibit any visible malformation of plant architecture, such as reduced growth and disrupted organogenesis, which are frequently observed when sold-responsive genes are overexpressed.
과제명(ProjectTitle) : | - |
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연구책임자(Manager) : | - |
과제기간(DetailSeriesProject) : | - |
총연구비 (DetailSeriesProject) : | - |
키워드(keyword) : | - |
과제수행기간(LeadAgency) : | - |
연구목표(Goal) : | - |
연구내용(Abstract) : | - |
기대효과(Effect) : | - |
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