초록 ▼

Ⅳ. 연구개발결과
T-DNA 삽입변이체 1,000 계통 분석하여 15개의 수량성 유망 유전자 확보하였으며 T-DNA 활성화 변이체 100 계통 분석하여 5개의 수량성 유망 유전자 확보하였다. 이삭 발달과 수량성과 연관이 있는 유전자 (SNB/OsIDS1, OsVIL2, OsJMJ703, SH5, OsTrx1) 의 기능을 연구하여 논문 5건 발표하였으며 총 3건의 관련 특허를 출원하였음. 또한 이삭발달 연구를 위한 키타케 T-DNA tagging line 분석하고 논문발표함. Bgl, Nal2/3, Rfs, Z2, Z3, Fgl

Ⅳ. 연구개발결과
T-DNA 삽입변이체 1,000 계통 분석하여 15개의 수량성 유망 유전자 확보하였으며 T-DNA 활성화 변이체 100 계통 분석하여 5개의 수량성 유망 유전자 확보하였다. 이삭 발달과 수량성과 연관이 있는 유전자 (SNB/OsIDS1, OsVIL2, OsJMJ703, SH5, OsTrx1) 의 기능을 연구하여 논문 5건 발표하였으며 총 3건의 관련 특허를 출원하였음. 또한 이삭발달 연구를 위한 키타케 T-DNA tagging line 분석하고 논문발표함. Bgl, Nal2/3, Rfs, Z2, Z3, Fgl, Spl2, Spl3, Spl4, Spl6, Chl4, Nal1 등 12 종의 유전자 지도기초분리 및 기능 연구 완료 후 논문 9 건 발표. 16종의 과발현 형질전환체 개발 후 형질전환체의 스트레스 저항성 및 수량성 테스트를 완료하였음.

Abstract ▼

Sub-project 1: Discovery and functional study on genes for increasing rice yield through analyzing panicle development-related genes
Rice is one of the world's three staple crops and that is preferentially cultivated in Asia. World population has been continuously increased but rice production ha

Sub-project 1: Discovery and functional study on genes for increasing rice yield through analyzing panicle development-related genes
Rice is one of the world's three staple crops and that is preferentially cultivated in Asia. World population has been continuously increased but rice production has been gradually declined by loss of agricultural land and unusual weather. Thus, study for increasing rice yield is very important. Rice production is determined by architecture of panicle, branch number and grain number. Thus, we hypothesized that genes involved in panicle development would be also concerned with increasing rice yield. In this study, we selected 1,100 genes, which are preferentially expressed in early panicle stage, using public database and Agilent 44K microarray. And then, we planted 1,000 T-DNA insertional lines and 100 activation tagging lines and made 6 overexpressing transgenic lines. Through analyzing the phenotypes and genotypes of mutant lines, we found seven yield increased lines and twelve yield decreased lines. miR172 and AP2 genes were involved in flowering inhibition and panicle branch development. OsJMJ703 encodes a histone demethylase for histone 3 lysine 4 (H3K4) and that positively controlled various developments such as plant height, panicle number, panicle length and seed size. SH5 encodes a BEL1-type homeobox transcription factor and that negatively controlled shattering by prompting lignin biosynthesis in abscission layer. OsVIL2 is a VIN3-like protein and that prompted floral transition by suppressing OsLFL1 with OsEMF2b. Especially, yield production of OsVIL2 OX plants were increased more than 20% and yield production of osvil4 were also increased more than 10%. Further study of OsVIL family would help increase rice production.
Collaborative-project 1: Map-based cloning and functional study of genes for increasing rice yield
In the paddy field, the bright green leaf (bgl) mutants of rice (Oryza sativa) exhibit a luminous green color that is clearly distinguishable from the normal green of wild-type plants. Transmission and scanning electron microscopy revealed that small cuticular papillae (or small papillae; SP), nipple-like structures, are absent on the adaxial and abaxial leaf surfaces of bgl mutants, leading to more direct reflection and less diffusion of green light. Map-based cloning revealed that the bgl locus encodes OsRopGEF10, one of eleven OsRopGEFs in rice. The zebra2 (z2) mutant in rice (Oryza sativa) produces leaves with transverse pale-green/yellow stripes. It was recently reported that ZEBRA2 encodes carotenoid isomerase (CRTISO) and that low levels of lutein, an essential carotenoid for non-photochemical quenching, cause leaf variegation in z2 mutants. However, we found that the z2 mutant phenotype was completely suppressed by growth under continuous light (CL; permissive) conditions, with concentrations of chlorophyll, carotenoids and chloroplast proteins at normal levels in z2 mutants under CL. NADPH:protochlorophyllide oxidoreductase (POR) catalyzes photoreduction of protochlorophyllide (Pchlide) to chlorophyllide in chlorophyll (Chl) synthesis, and is required for prolamellar body (PLB) formation in etioplasts. Rice faded green leaf (fgl) mutants develop yellow/white leaf variegation and necrotic lesions during leaf elongation in field-grown plants. Map-based cloning revealed that FGL encodes OsPORB, one of two rice POR isoforms. In fgl, etiolated seedlings contained smaller PLBs in etioplasts, and lower levels of total and photoactive Pchlide. In order to understand the molecular genetic mechanisms of rice (Oryza sativa) organ development, we studied the narrow leaf2 narrow leaf3 (nal2 nal3; hereafter nal2/3) double mutant, which produces narrow-curly leaves, more tillers, fewer lateral roots, opened spikelets and narrow-thin grains. We found that narrow-curly leaves resulted mainly from reduced lateral-axis outgrowth with fewer longitudinal veins and more, larger bulliform cells. Opened spikelets, possibly caused by marginal deformity in the lemma, gave rise to narrow-thin grains. Map-based cloning revealed that NAL2 and NAL3 are paralogs that encode an identical OsWOX3A (OsNS) transcriptional activator, homologous to NARROW SHEATH1 (NS1) and NS2 in maize and PRESSED FLOWER in Arabidopsis. OsWOX3A is involved in organ development in rice, lateral-axis outgrowth and vascular patterning in leaves, lemma and palea morphogenesis in spikelets, and development of tillers and lateral roots. Futhermore, we investigated narrow leaf1 (nal1), a pleiotropic mutant with short and narrow leaves, semi-dwarf stature, and fewer adventitious (or crown) roots. The narrow leaf5 (nal5) and nal1 mutants display similar defects in leaf and root development.
Sequence analysis and complementation tests showed that nal5 is allelic to nal1. NAL1 encodes a putative trypsin-like serine/cysteine protease; the coding region of nal5 contains a missense mutation and nal1 harbors a 30-bp deletion.

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 요 약 문 ... 4
• SUMMARY ... 5
• 목차 ... 7
• 제 1 장 서 론 ... 8
• 제1절 연구개발대상 기술의 경제적ㆍ산업적 중요성 및 연구개발의 필요성 ... 8
• 제 2 장 국내외 기술개발 현황 ... 12
• 제1절 국내 연구 현황 ... 12
• 제2절 국외 연구 현황 ... 13
• 제3절 국내외 연구현황 비교 및 필요 연구 분야 ... 14
• 제 3 장 연구개발수행 내용 및 결과 ... 16
• 제1절 이삭 발달 유전자 분석에 의한 수량성 증대 벼 유용 유전자 기능 연구 ... 16
• 제2절 벼 수량성 유전자 지도기초분리, 생체기능 구명 및 유용유전자 형질전환 ... 77
• 제 4 장 연구개발목표 달성도 및 대외기여도 ... 121
• 제1절 세부과제별 정성적 연구목표대비 달성도 ... 121
• 제2절 정량적 목표대비 달성도 ... 122
• 제 5 장 연구개발결과의 활용계획 ... 127
• 제1절 추가연구의 필요성, 타 연구에의 응용, 기업화 추진방안 ... 127
• 제2절 현재 추진 중인 추가적인 논문게재, 산업재산권 출원 사항 ... 128
• 제 6 장 연구개발과정에서 수집한 해외과학기술정보 ... 129
• 제 7 장 기타 중요 변동사항 ... 131
• 제 8 장 국가과학기술종합정보시스템에 등록한 연구장비현황 ... 132
• 제 9 장 참고문헌 ... 133
• 끝페이지 ... 138