초록

Ⅳ. 연구개발결과
-전통육종에 분자마커를 활용한 선발 기술 등의 첨단 기술을 활용한 분자육종의접목이 이루어졌으며, 보다 많은 분자마커를 저비용, 고효율로 분석할 수 있는 시스템의 개발을 수행함.
- 고추와 토마토에서 저항성 Multiplex 유전형 분석 키트 각각 개발함.

Abstract ▼

Next-generation sequencing (NGS) technology and fast improvement in plant genetics have elevated to massive development of molecular genetic markers through fast analysis of huge molecular biological data. Furthermore, in domestic commercial breeding of horticultural crops, the application of marker

Next-generation sequencing (NGS) technology and fast improvement in plant genetics have elevated to massive development of molecular genetic markers through fast analysis of huge molecular biological data. Furthermore, in domestic commercial breeding of horticultural crops, the application of marker assisted breeding (MAB) has been introduced recently. For effective improvement of cultivar breeding, in this research, transcriptome analysis and single nucleotide polymorphism (SNP) comparison with high density pepper map in UC-DAVIS were performed using four lines of Capsicum annuum and C. chinense. For rapid analysis of MAB of tolerant pepper lines, 412 Fluidigm probes were newly designed in this study. These designed probes and SSR and COSII markers were applied for background selection through the MAB program. In addition, powdery mildew (PM), tomato spot wilt virus (TSWV) resistance related markers were subjected to foreground selection of BC1, BC2, and BC3 progenies. The MAB system using Fluidigm probes, and trait-related and common markers was introduced into domestic pepper breeding, which will rapidly approach to a new elite line and a commercial tolerant cultivar.
Recent breeding technology is using molecular markers for genotyping of useful gene. Many breeders are generally using the MAS (Marker assisted selection) marker for selecting objective characters. On the other hand, the MAB (marker assisted backcross) marker that using SNP (Single nucleotide polymorphism), is not commonly used. SNP marker is reported that related with metabolism, function, characteristics, pathogen resistance, according to its location. Therefore, scientists are research to find the new SNP location with function. Based on the SNP information that has various pathogen resistances for pepper and tomato, we developed the test kit with multiplex PCR. In case of the kit for pepper, the SNP information that has pathogen resistance of tobacco mosaic virus (TMV), Bacterial spot (BS), Chilli venial mottle virus (ChiVMV), Phytophthora blight (Phyto) were used. And for tomato, the SNP information that has pathogen resistance of meloidogyne incognita (Mi), tomato mosaic virus (ToMV), tomato yellow leaf curl virus (TYLCV), leaf mold (Cf9), fusarium wilt (I2) were used.
The test kit is appropriate to be applied for small-sized nursery company and private breeders who cannot afford MBA analysis that needs expensive machine and reagents. And furthermore, by reducing breeding time, early promoted superior cultivar would help to grow national competitiveness.