보고서 정보
주관연구기관 |
세종대학교 Sejone university |
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 |
한국어
|
발행년월 | 2015-02 |
과제시작연도 |
2013 |
주관부처 |
농촌진흥청 Rural Development Administration(RDA) |
등록번호 |
TRKO201500010581 |
과제고유번호 |
1395031049 |
사업명 |
차세대바이오그린21 |
DB 구축일자 |
2015-07-11
|
DOI |
https://doi.org/10.23000/TRKO201500010581 |
초록
Ⅳ. 연구개발결과
◇ 설프라펜 고함유 F1(대양 1호) 품종보호출원
◇ 육질이 치밀하고 당함량이 높은 F1(탑동) 품종 보호출원
◇ 품종보호출원 2품종 기술이전으로 농가 소득증대 예상
◇ 4.0±0.5mm 길이의 화뢰에서 소포자 유래 배 발생 효율이 가장 높음
◇ 품종 및 계통간 F1의 설프라펜 성분량이 현격한 차이가 있음을 확인
Abstract
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[1세부 : 기능성무 품종육성 및 소포자 유래 계통 이용 품종 육성]
Radishes (Raphanus sativus L.), a brassica family together with Chinese cabbage, are widely grown in Korea and Japan. It contains various vitamins, inorganic materials and sulfur compounds as alkali vegetable. Moreover, it also contains protein, fat, sugar,
[1세부 : 기능성무 품종육성 및 소포자 유래 계통 이용 품종 육성]
Radishes (Raphanus sativus L.), a brassica family together with Chinese cabbage, are widely grown in Korea and Japan. It contains various vitamins, inorganic materials and sulfur compounds as alkali vegetable. Moreover, it also contains protein, fat, sugar, fiber, vitamin, iron, magnesium, potassium, and much more of functional materials. It has high commercial value which is one of 7th top selling and producing vegetable crop in Korea. Domestic production is about 35,000ha, which is 10% of total vegetable production area, and it is grown in 45,000ha in Japan. All together in China, India and South East Asia, these are grown in 1,500,00ha. Therefore, these important crops need to be improved in their use not only for Kimchi ingredient, but also high functional materials for higher cash value.
To breed new radish with high functional materials, the parental lines were developed by 5~7 times of selections, and crossings were made by using these fixed lines. F1 hybrids were further developed in the field test crossing. Those F1 hybrids which have high sulphoraphene were selected and further named DaeYang-1, and applied for PVP.
To shorten the breeding periods in radish, microspore culture was applied for seed production, which were later field tested and further used in crossing for F1. Moreover, to develop anthocyanin-rich radish, new F1 hybrids were made by crossing with high anthocyanin-containing parental lines.
Therefore it was proven that using microspore culture has shortened the breeding periods. Also, it showed that development of anthocyanin rich radish can be delivered bycrossing high anthocyanin containing parental lines.
[1협동 : 육종연한 단축을 위한 무 소포자유래 식물체 유기]
Composition of nutrient media, flower bud size, sucrose concentration, heat shock stress, and ethylene inhibitor could have marked effects on microspore embryogenesis. No microspore-derived embryos (MDE) were formed when microspores were isolated from radish (Raphanus sativus L.) flower buds of 2.0 mm in size, whereas MDE were formed with microspores isolated from 4.0 and 6.0 mm flower buds. The microspores isolated from 4.0 mm flower buds showed high embryo yields. MDE formation was highest when 150 g L-1sucrose was added to the Nitsch & Nitsch (NLN) liquid medium, but at sucrose concentrations less than 100 g L-1there was no MDE formation. Microspores cultured on half strength NLN liquid medium containing 0.05 mg L-1silver nitrate (AgNO3) produced the most MDE, showing a more than two-fold increase in yield compared to those cultured on medium without AgNO3. A heat shock pretreatment of microspores at 32°C for 24 hours gave high-frequency production of MDE when compare to higher or lower temperatures; no MDE were formed at 42.5°C. The highest yield of MDE was observed when microspores were derived from 4.0 mm flower buds cultured on half strength NLN medium containing 150 g L-1 sucrose, 0.05 mg L-1 AgNO3, and precultured with heat shock pretreatment of microspores at 32°C for 24 hours. The yield of MDE increased significantly when microspore cultured for 15 days at 25℃ in dark condition followed by heat shock pretreatment. MDE were browned and lost vitality when dark treatment period extended longer than 15 days. This is caused by an insufficient oxygen and light for growing embryo which already formed during dark treatment period.
This shows the possibility of getting a certain period of storage for a suitable flower bud in MDE formation. The yield of MDE was most effective when isolated microspore was washed with MS medium compared to B-5 and NLN medium and also showed most effective result with sucrose 130 gㆍL 1 in additional sucrose concentration. Investigation of flowing time, flower structure, microspore density, microspore vitality and microspore derived embryo formation rate according to the light quality treatment on radish donor plant was accomplished. The donor plant was cultivated with light quality treatment of red LED light, red+blue+white LED light and fluorescent light. The light intensity was 50 μㆍmolㆍm-1ㆍs-1and photoperiod was 16/8 hours (light/dark). There was no the flower structural difference according to light quality. The flowering time was fastest at red LED light treatment compared to the other light treatment condition. The microspore density, microspore vitality and MDE production rate per single flower bud was highest at Red LED light. Suitable flower bud and high MDE production rate could be achieved in a short period if using LED light to radish donor plant cultivation. The above result is thought to be very useful for the development of a new cultivar of broccoli and other many crops including brassica using haploid breeding technology.
[2협동 : 기능성무 함량증가를 위한 재배법 개발 및 성분 분석]
1. Effects of different colored mulching on radish growing and soil temperature
The effects of mulching are known as soil temperature control, weed control, soil moisture management, soil nutrition holding and protection from insects. By increasing the root development and nutrition use, the mulching methods increase the crop yield. This research will show the yield difference and effect for soil temperature, germination and bolting by applying various mulching film colors such as black, green, transparent and no-mulching.
1-1. The effects of black, green and transparent mulching film on autumn - and overwinter-type radish growing
Oriental radish (OR) are grown throughout the year, and mulching methods are widely used. However, the effects of mulching in different growing stage are unknown. Therefore, this research delivers the effect of various mulching film use on autumn and verwinter oriental radish growing. In this research, the autumn oriental radishes were grown in Syngenta RandD, Icheon, Gyeonggi-do, and overwinter overwinter-type radish were grown in Seoguipo, Jeju-si. Each group consists of green, black, transparent film mulching with non-mulching plots. Sowing date for 14 autumn-typel radish hybrids was in Aug.28.2013 and for 10 overwinter-type radish was in Sep.12.2013. Data collection for root length, root diameter (upper, middle, lower), root weight, leaf length, leaf numbers, and leaf weight of 10 varieties with 2 replications were conducted in Nov.13.2013 for autumn and Jan.14.2014 for overwinter-type radish. Data were analyzed with SPSS12.0 and the result shows distinguishable effects on mulching. The effect on root diameter (middle), leaf length, leaf numbers, root weight, leaf weight were increased in autumn-type radish, and root weight, leaf weight were increased yet root length and weight showed no relation in overwinter-type radish compare to non-mulching methods.
The transparent and green film color increased the root diameter (middle) and root weight in autumn-type radish. Leaf numbers were higher in non-mulching yet black film mulching decreased leaf weight. Therefore, the effect of mulching film colors are depends on growing stages, leaf and root parts.
1-2. The mulching effect on soil temperature, germination and bolding in Oriental radish
Mulching methods are effective on temperature and water contents in soil. Depends on mulching film color, it reflects or absorbs the radiant heat. In the night, the mulching reduces heat and water loss, so it helps the consistency of water and heat in the soil. This research delivers the effects of soil temperature, germination and bolting rate by applying green, black transparent and no mulching methods. 6 Oriental radish varieties were directly sown at the field of Syngenta RandD, Icheon, Gyeonggi-do in April. 11. 2014. Analysis on germination rate was delivered in April. 30, and 10 entries with 3 replications in each variety of bolting, root length, root diameter (upper, middle, lower), leaf length, leaf numbers, root weight and leaf weight were collected on harvesting in Jun. 20. To monitor the soil and air temperature, digital thermometer (TR-71UI, TandD Corporation, Japan) were placed in the field at the sowing. The average soil temperature was high with transparent>black>green>no-mulching film in early stage (20 days after sowing). In middle stage (20 days to 40 days after sowing), the temperature was high with transparent>green>black>no-mulching film. In late stage (40 days to 70 days after sowing), there were no big differences. The highest daily temperature was high with transparent and black film in early stage. However, the effects on latest age were not augment because the radish leaves covers the surface of mulching film. This may cause the prevention of radiation reaching the soil and hold the accumulated heat in the night.
The germination rate was increased in non-mulching. The bolting time by different film color was fast in non-mulching > black ≥ green > transparent. Soil temperature and bolting time showed negative correlation.
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