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Kafe 바로가기주관연구기관 | 국립농업과학원 National Institute of Agricultural Sciences |
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보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 | 한국어 |
발행년월 | 2015-02 |
과제시작연도 | 2014 |
주관부처 | 농촌진흥청 Rural Development Administration(RDA) |
등록번호 | TRKO201500010598 |
과제고유번호 | 1395038059 |
사업명 | 친환경안전농축산물생산기술 |
DB 구축일자 | 2015-07-11 |
DOI | https://doi.org/10.23000/TRKO201500010598 |
Ⅳ. 연구개발결과
1. 선정 유용미생물의 최적 배양 조건 구명하여 일반적으로 사용하는 배지와 대등한 성장을 보이는 저가의 실용배지 4종을 개발함(BSM, PSM, YSM, LSM).
2. 유용미생물 배양액의 농도를 현장에서 쉽게 측정할 수 있는 간이 밀도 측정법을 개발하여 영농활용에 등록함
3. 유용미생물의 지역별, 작목별 최적 활용조건을 분석하여 현장에서 활용할 수 있도록 정리하였으며 이를 책으로 발간하여 150여 개 시군농업기술센터에 보급함
4. 참외, 고추에서 미생물의 농도, 처리주기, 처리량에 따른 생육
Ⅳ. 연구개발결과
1. 선정 유용미생물의 최적 배양 조건 구명하여 일반적으로 사용하는 배지와 대등한 성장을 보이는 저가의 실용배지 4종을 개발함(BSM, PSM, YSM, LSM).
2. 유용미생물 배양액의 농도를 현장에서 쉽게 측정할 수 있는 간이 밀도 측정법을 개발하여 영농활용에 등록함
3. 유용미생물의 지역별, 작목별 최적 활용조건을 분석하여 현장에서 활용할 수 있도록 정리하였으며 이를 책으로 발간하여 150여 개 시군농업기술센터에 보급함
4. 참외, 고추에서 미생물의 농도, 처리주기, 처리량에 따른 생육과 수량
5. 미생물 처리에 따른 흰가루병 등 병해억제효과와 축사에서 악취 감소효과
6. 양파 생육기인 3월 상순부터 7일 간격으로 Pseudomonas sp.와 Kluyvera sp.를 100배액 (109 cfu/mL기준)으로 희석하여3톤/10a씩 5회 토양에 관주하였다.
○ 수량은 무처리에 비해 8∼11% 증가
7. 고추 탄저병 방제에 30%∼57.9% 방제효과 있으며, B. amyloliquefaciens CC178 (KACC91292)가 Bacillus sp.(KACC91195)에 비해 방제효과 높음
8. 파프리카 흰가루병 방제에 Bacillus sp.(KACC91195)는 무처리 대비 88.4%효과가 있으며, Bacillus amyloliquefaciens CC178(KACC91292)는 무처리 대비 20.7% 효과가 있음
9. 딸기 잿빛곰팡이병 방제에 KACC91292 처리시 31.3% 방제효과를 나타냄
10. 고추생육촉진 효과는 KACC91281 처리시 고추 생육촉진 효과가 있었으며, 관주처리방법이 가장 효율적임
11. 양상추 생육촉진 효과는Bacillus valliemorits(KACC11991) 100배액 3회 처리시 무처리 대비 6% 증수
12. 토양 중 특성변화는 농업미생물 처리구 토양 인산화 가용화율 증가함
13. 미생물 처리구(KACC91282, KACC91283) 29.6〜32.5% 수량 증가, 비상품과 수량을 제외한 건고추 수량, 홍고추 수분함량 81.53%
14. 재래식 및 슬러지 돈사에서 미생물 처리후 암모니아와 아민류 가스의 감소
15. 슬러지 돈사에서 미생물 처리후 가스변화는 NH3 5, R-NH2 16 ppm 감소
16. 유용미생물을 이용한 고추탄저병 방제 활용 기술 개발
17. P. stutzeri NIST-1 균주를 원액으로 살포한 경우 약 30.8%의 저감효과를 보였음
18. Bacillus subtilis S37-2 균주는 유효인산의 집적이 심한 시설 연작재배지
Many beneficial microorganisms have been developed and used as eco-friendly agriculture materials for biocontrol against plant diseases, growth promotion of crops, and improvement of agricultural environment such as phosphate-accumulating soils and livestock facilities. We developed the low-cost mic
Many beneficial microorganisms have been developed and used as eco-friendly agriculture materials for biocontrol against plant diseases, growth promotion of crops, and improvement of agricultural environment such as phosphate-accumulating soils and livestock facilities. We developed the low-cost microbiological media to cultivate efficiently them in the field. Simple methods for measurement of density of microorganisms in the culture broth were made by using spectrophotometer. we published the protocol for practical application of agricultural microorganisms. We expected that this book would give many helps to persons in charge of distribution of microorganisms and farmers.
We conducted identification for the effect of promoting the plant growth of microorganism of lettuce and tomato. Lettuce growing in the spring, yield is greater at KACC 91283 than non-control treatment, and commercial yield is greater at treatment of KACC 91283 than the others microorganism. In the autumn culture of lettuce, yield is greater than non-control treatment at KACC 91283, and they similar to the spring culture. In the tomato cultivation under structure, yield is greater than control treatment at KACC 91282, KACC 91283, and commercial yield is greater than the others microorganism at KACC 91283 and commercial yield is greater than control at KACC 91283.
To search the soaking effect of microorganism suspension on several kinds of seeds, lettuce, chinese cabbage, cucumber, and tomato seeds were immersed on suspension for 2 days, and they were seeded on pot in plastic house. The sprouting and growth of every seed that microorganism suspension were soaked were markedly suppressed indicating not proper method. For the study of disease protection effect of microorganism, No. 91281 isolate was cultured in potato dextrose broth for 3 days, and diluted to 1×106, 5×106, 1×107 per ml. When diluted solutions were sprayed at 3, 6, 9 days intervals to oriental melon, 1×107 concentration was effective to powdery mildew showing around 62% control efficacy at 3 and 6 days interval spraying. But there was no effect by treatment of lower concentration than 1×107 per ml. So, it is expected that KACC 91281 isolate is useful to control of powdery mildew in organic cultivation of oriental melon. To reduce odor using microorganism in the livestock raising, KACC 91343 isolate were sprayed to raising facility of cow and pig, and ammonia gas concentration was checked. Ammonia concentration was significantly reduced by spraying of 91343 suspension compared to untreated area. It must be effective to reduce order but required more experiment to be used.
Three species of microorganisms, Bacillus subtilis(KACC 91281), Pseudomonas sp.(KACC 91282) and Kluyverasp.(KACC 91283), growth-promoting effects such as phosphate solubilization were drenched in soil six times at 7-day intervals in Chinese cabbage growing season. Yield of Chinese cabbage increased 9-13% in the microbial agent treatments compared to control.
In organic onion farming, it is necessary to develop a technology to improve low production compared to yield of practice cultivation. Pseudomonas sp and Kluyvera sp. were drenched in soil 5 times at 7-day intervals from early March in onion growing season. Microbial agents were irrigated 3 tons per 10a at a concentration of 107 cfu/mL. Yield of onion increased 8-11% in the microbial agent treatments compared to control. Application of agricultural microorganism should be considered as a useful way to increase production of vegetables such as chinese cabbage and onion crops.
We have performed experiment for checking of effect on microorganism such as plant disease control efficiency, plant growth promoting efficiency, and livestock manure odor removal efficiency ect. In plant disease control efficiency, the microorganism including Bacillus amyloliquefaciens CC178(KACC 91292) and Bacillus sp.(KACC 91195) have effected for control of plant disease. In plant growth promoting efficiency, Bacillus subtillius S37-2 (KACC 91281) have effected on pepper with irrigating of microorganism, and Bacillus valliemorits(KACC11991) with 3 times of 100 diluent have more effected growth promoting efficiency on lettuce. In soil phosphate solubilizing efficiency, the soil phosphorylation solubilization rate was increased as the treated microorganisms and Biomass C and dehydrogenase activity was also high. In livestock manure odor removal efficiency, microbial treatment after the gas changed in the conventional nursery as R-NH2 4.9 ppm decrease. In the microbial treatment after the gas changed in the piggery sludge, NH3 and R-NH2 was decreased 5, 16 ppm, respectively.
To investigate the growth promotion of strawberries, the S37-2 culture solution was drenched in eight times and surveyed twice in one month intervals. As a result, the best treatment for the growth promote of plant height and petiole was appropriate amount plot. Anthracnose caused by Colletotrichum acutatum was reported to be severe disease on chili pepper (Capsicum annuum). Disease progress of anthracnose was investigated during the growing season of 2010 to 2013 at Yesan area. The anthracnose disease on chili pepper fruits began to occur to late May and peaked in late August with 15.3~93.1%. The antifungal bacteria, Bacillus sp. YJH-051 isolate suppress the anthracnose with 60.9% seven times sprayed application of bacterial cultures control efficacy, while that of three and five times spray was 39.1 and 52.2% respectively.
Effect of phosphate solubilizing bacteria(Kluyvera sp. CL2) application on tomato in greenhouse were as follows. Phosphate solubilization rate of tomato was highest in the concentration 1.0×107cfu/g(73.3%). Yield of tomato fruits was highest in the concentration 1.0×107cfu/g(7,611kg/10a). Soil treatment with Kluyvera sp. CL2 resulted into up to 9% increment in yield and 7% increment in soluble solids during 4 month period.
Control effect of three bacteria, Bacillus amyloliquefaciens CC178, Bacillus sp. YJH-051 and Pseudomonas stutzeri NIST-1, against powdery mildew of pepper was examined in seedling test. Under plastic house, control effect against powdery mildew of pepper was examined. The antifungal bacteria were sprayed at a concentration of 106 and 109cell/ml with intervals of 7 days. Bacillus amyloliquefaciens CC178 and Bacillus sp. YJH-051 were more effective in controlling powdery mildew of pepper when the spray began before disease occur. Especially in treatment of Bacillus sp. YJH-051 with a concentration of both 106, powdery mildew was significantly reduced which was similar in chemical, tetraconazole.
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