초록

Ⅳ. 연구개발결과
1) 단일세포 레벨의 DNA, RNA 분석이 가능한 실험 및 분석 파이프라인을 성공적으로 설치, 최적화시킴.
2) 단일세포 분석을 통해서 종양의 유전적 특징을 세포 단위로 분류하고 약물치료 저항성 및 감수성을 예측함
3) 임상 샘플 분석 노하우를 활용하여 단일세포 분리 기술과 유전체 분석 기술의 unmet needs를 파악하고 관련된 제품 디자인 완료

Abstract ▼

Contents and Results
1) Implementation of single cell capture and analysis pipelines
Single tumor cells were isolated and its DNA or RNA amplified in FACS-MDA and C1_Smartseq process. Single cell whole genome sequencing and RNA sequencing analysis pipelines are implemented.
2) Clinical appl

Contents and Results
1) Implementation of single cell capture and analysis pipelines
Single tumor cells were isolated and its DNA or RNA amplified in FACS-MDA and C1_Smartseq process. Single cell whole genome sequencing and RNA sequencing analysis pipelines are implemented.
2) Clinical application of the single cell analysis
-Single cells from diverse tumor specimens (Breast cancer, Lung adenocarcinoma, Renal cell carcinoma) were captured and subjected to RNA sequencing. Heterogeneity in drug resistance signatures and drug sensitive signatures were analyzed and matched to experimental data.
-Circulating tumor cells from lung adenocarcinoma patients were captured in the magnetic device for the feasibility test.
3) Design of single cell capture device
-Magnetic system was tested to capture the spike-in cell lines to mimic CTCs. The performance required improvement and we designed a new device using microfluidics.