초록 ▼

Ⅳ. 연구개발결과
- 국화, 양파, 배의 세포질 유전체인 14개의 신규 엽록체 완전 염기서열 NABIC에 등록함. 이들 원예작물의 근연종인 국화과, 장미과, 비짜루 목의 다수 작물에 대한 엽록체 유전체를 분석하였음.
- 원예작물인 국화, 양파, 배의 미토콘드리아 유전체의 90~100% 유전자 염기서열을 확보함
- 작목당 16개이상의 품종/근연종의 엽록체유전체의 79~80개의 단백질 코딩유전자를 이용하여 모계계통진화를 연구하였음
- 국화, 양파, 배를 포함하는 원예작물과 그 근연 20여 종류의 NGS data를

Ⅳ. 연구개발결과
- 국화, 양파, 배의 세포질 유전체인 14개의 신규 엽록체 완전 염기서열 NABIC에 등록함. 이들 원예작물의 근연종인 국화과, 장미과, 비짜루 목의 다수 작물에 대한 엽록체 유전체를 분석하였음.
- 원예작물인 국화, 양파, 배의 미토콘드리아 유전체의 90~100% 유전자 염기서열을 확보함
- 작목당 16개이상의 품종/근연종의 엽록체유전체의 79~80개의 단백질 코딩유전자를 이용하여 모계계통진화를 연구하였음
- 국화, 양파, 배를 포함하는 원예작물과 그 근연 20여 종류의 NGS data를 확보함

Abstract ▼

Cytoplasmic genomes of 48 horticultural crops and their wild relatives including Denranthema spp. (=Chrysanthemum) , Allium spp. and Pyrus spp. have been characterized as a research project supported by National Agricultural Genome Center. So far, more than 30 chloroplast genomes were completely seq

Cytoplasmic genomes of 48 horticultural crops and their wild relatives including Denranthema spp. (=Chrysanthemum) , Allium spp. and Pyrus spp. have been characterized as a research project supported by National Agricultural Genome Center. So far, more than 30 chloroplast genomes were completely sequenced, and fourteen plastid genome sequence were deposited in NABIC. Chloroplast genomes of Dendranthema spp. were 151,017~151,175-bp in size; LSC 82,821~82,886, IR 24,954~24971, and SSC 18,282~18,347. Chloroplast genomes of chrysanthemum cultivars (Chrysanthemum grandiflorum) were similar to that of Denranthema boreale, than that of other 4 Denranthema spp. Chloroplast genomes of Pyrus spp. were 159,884~159,992-bp in size; LSC 87,862~87,892, IR 26,392, and SSC 19,237~19,238. Pyrus cp genomes are 2~4kb bigger than other known Rosaceae. Chloroplast genome of Chinese pear, P. bretschneideri CV Yali, was similar to that of Pyrus pylifoia CV Hosui, by having same gene sequence and genome structure, except for variation in IGS regions and introns. Chloroplast genomes of Allium spp. were 153,216~151,586-bp in size; LSC 82,395~82,699, IR 26,466~26,478, and SSC 17,825~17,931. Among the 4 MS lines of A. cepa showed variation in genes, introns, and IGS. These include SNPs in genes, monomeric and large repeats in introns, and SNPs, large INDELs, inversions in IGS. For Allium, Dendranthema and Pyrus, 90% of mitochondrial genes were characetrized. Phylogenies of the three crops were investigated using all protein coding sequences. This research incorporated with cytological and nuclear genome researches will give us useful information in MAS and better understanding in crop evolution.