초록 ▼

IV. 연구개발결과
1. 발효사료 첨가제 위탁생산 체제 구축
1) 종균관리시스템 관련, 동결건조 공정 개선 (적정 호제 종류 및 능도 결정
2) 소량다품목 대량 위탁생산설비 보완 : 액상배양기 및 드럼형 발효기/발효실 설비
3) 각종 부산물을 이용한 고체발효 배합비 규명
4) 3종 미생물에 대한 고효율 액상배양 조건 규명 및 적합 범용 배지 조성
5) 맞춤형 고체 발효공정 완비
6) 위탁생산 실시 : 축협, 영능법인, 조합, 기업 (2014년 시작, 2015년 4곳)
2. 유용미생물을 이용

IV. 연구개발결과
1. 발효사료 첨가제 위탁생산 체제 구축
1) 종균관리시스템 관련, 동결건조 공정 개선 (적정 호제 종류 및 능도 결정
2) 소량다품목 대량 위탁생산설비 보완 : 액상배양기 및 드럼형 발효기/발효실 설비
3) 각종 부산물을 이용한 고체발효 배합비 규명
4) 3종 미생물에 대한 고효율 액상배양 조건 규명 및 적합 범용 배지 조성
5) 맞춤형 고체 발효공정 완비
6) 위탁생산 실시 : 축협, 영능법인, 조합, 기업 (2014년 시작, 2015년 4곳)
2. 유용미생물을 이용한 발효 사료 첨가제 개발
1) 미생물 범용 배양배지 4종 업그레이드 국 자가배양능가 능업법인 둥에 공급
2) 가금티푸스 원인균 길항미생물 이용 가금용 생균제 개발 - 육계 사양실험 완료 → 특허등록
3) 효소활성, 병원균 길항미생물을 이용한 사료첨가제 개발
4) 복합발효 효소제 개발, 제품출시 및 중국내 사양실험 완료 극 중국 수출 추진중
5) 생물전환을 통한 생리활성물질의 사료첨가제 개발 - 육계 대상 효과검증 완료
: 항산화물질 둥의 생리 활성 발효사료 첨가제 개발 국 특허출원 및 사업화 계획중
6) 자가배양 또는 TMF용 발효종균제 업그레이드
3. 위탁생산을 위한 고효율 액상 및 고체발효 조건 규명
1) 대표적 유용미생물 3종에 대한 대량배양용 범용 배지 조성
2) 액상배양물의 생존 연장을 위한 적정 보존재 및 처리 능도 결정
3) 유용미생물 동결건조체의 재생를을 증진시키기 위한 적정 동결억저제 및 농도 결정
4. 유용미생물을 이용한 발효 사료첨가제 개발 및 효과 검증을 위한 지표 설정
1) 유용미생물 확립 및 조건 규명
2) 고효율 발효사료첨가제 제조 공정기슬 확립 :원료 종류별 제조 공정기슬 확립
3) 기존의 발효 사료첨가제의 효능 평가 지표로 생균수 분석외 추가 평가지표 설정
5. 개발된 발효 사료첨가제의 효과 검증과 급여체계 확립
1) 가금티푸스 길항미생물을 이용한 발효 사료첨가제의 육계에 대한 효과 검중
2) 유용미생물 혼합 발효를 이용한 발효사료첨가제의 한우에 대한 효과검증
- 어린 송아지, 육성우, 비육우,
3) 유용미생물 혼합 발효를 이용한 발효사료첨가제의 돼지에 대한 효과검증
- 이유자돈, 육성돈, 비육돈

Abstract ▼

Construction of Nongovernmental CMO oi iermented ieed adailives
In order to establish an effective CMO(Contract manufacturing organiszation) system achieved the four objectives, as follows；
1. Standardization of seed microbial control has been established.
2. New model case of contract manu

Construction of Nongovernmental CMO oi iermented ieed adailives
In order to establish an effective CMO(Contract manufacturing organiszation) system achieved the four objectives, as follows；
1. Standardization of seed microbial control has been established.
2. New model case of contract manufacturing system for fermented feed additives on-demand was presented.(Several types of small amounts / Mass production)
3. Mass production process was improved and standardized to maximize the efficacy of the product and reduce production costs of fermented feed additives.
4. In addition to existing analysis of number of probiotic cells, there was supposed new analytical indicater candidates for the quality assurance of fermented feed additives.
5. Basic data and experiences were accumulated to advice in public organization like as agricultural cooperation that wish to self-made fermented feed additives.
Separately, it developed three kinds of fermented feed additives. Among them, fermented multi enzyme complex is scheduled for commercialization are currently exported to China.
Research on developing fermented feed additives using useful microorganism and verifying the effect of fermented feed
The knowledge for the cultivation technologies, medium composition, culture conditions and quality evaluation methods for production in low cost are lacked for supplying useful microorganism to livestock farmers. The production for supply of liquid and solid state fermentation microbial agents requires the field technology. The following studies were conducted to establish efficient production method of a useful microorganism along with the development of various excipients and formulation easy to apply to the field.
1. Establishing the useful microbial and identifying the conditions to develop fermented feedstuff additives.
-Establishing beneficial microorganisms such as lactic acid bacteria, bacillus, yeast, and photosynthetic bacteria to ferment plants and natural resources.
-Determining the condition of the liquid and solid state fermentations to develop fermented feed additives utilizing natural resources.
The microorganism with strong activities of digestive enzymes and anti-microbial activity were isolated from Korean cattle gastric juice, silage and seaweed powder. Feed fermentation was characterized by using lactic acid bacteria and bacillus strain, singly and mixedly in order to determine the liquid and solid state fermentation conditions.
2. Establishing high efficiency process technology to produce fermented feed additives
-Establishing processing technology according to materials of fermented feed additive.
-Establishing cultivation method through PBD (P1acke11-Burman design) analysis
-Manufacturing of trial product and establishing the method for formulation
The impact on the cultivation results by the strain selected for each material of feedstuff was surveyed. The optimal mixing condition was examined based on the results. The design method for mixes, one of partial factor analysis method was used for the optimal mixing condition, and its effect was examined through variance analysis and response value was modeled using optimized tool, Finally the optimal medium composition of different features was surveyed.
3. Setting the indicator for efficacy evaluation in addition to method to identify the count of microbial in probiotic bacteria/fermented feed
-The water content, the count of microbial, pH, preservability
-Biological activity (Measurement of antioxidant activity, antimicrobial activity, and enzymatic activity)
The basic activity of various physiological activities were measured and compared on the prototype of fermented feed using single and mixed culture method. The activity of dehydrogenase which is used as indicator to determine living microorganism of soil was applied as a new evaluation method together with measuring the count of microbial which is a method to evaluate the quality of conventional feedstuff at storage temperature, 15°C and 30°C.
○ Various useful strains for the fermentation of feed material were obtained from the byproducts of agricultural foods and it may be useful for the fermentation agricultural byproducts.
○ The lactic acid bacteria and bacillus in mixing in a MRS medium were cultured, and antibiotic resistance spectrum which can identify the two bacteria for the quality control was established.
○ Soybean meal of 3.96%, wheat bran of 19.76% and corn powder of 76.2 % were derived for the optimal compounding conditions to ferment feedstuff efficiently using various strains through manufacturing process technology and PBD(Plackett-Burman design) analysis on different types of material.
○ The result of measuring a variety of biological activities of the trial product manufactured confirmed that the mixed culture had high survival rate of lactic acid bacteria compared to the single culture.
○ The activity of dehydrogenase, which is a new method to evaluate quality of fermented feed additives showed more active in the mixed culture compared to single culture.
Effects of dietary supplementation of Fermented feed additive on growth performance, blood biochemical profiles and meat quality in broiler cickens, pigs and Hanvoo.
1. The effect of fermented diets on growth performance and Fatty acid profile of broiler are as follows；
(1) Fermented diet supplemented groups had numerically higher feed intake, body weight gain, average daily gain and feed efficiency than the control.
(2) Fermented diet supplemented groups had higher myristic acid (C14：0) concentration than control, but lower pentadecanoic acid (C15:0) concentration.
(3) Oleic acid (C1 :ln-9) concentration increased with increasing levels of fermented diet. 2
2. The effect of fermented diet on growth performance and blood metabolites of weaning pig are as follows；
(1) Final weight, gain, average daily gain and feed efficiency were numerically highest in HP treatment.
(2) Insulin, IgF-1 and blood glucose were highest 1% fermented diet (MP) treatment.
(3) On 3 weeks, Salmonella and E, coli were not detected in all treatments.
3. The effect of fermented feed additives on growth performance, blood metabolites and meat quality of growing pig are as follows；
(1) In 1.0% fermented diet (HP), feed intake and gain was lowest, but average daily gain was highest,
(2) Blood urea nitrogen and blood glucose were increased with increasing levels of fermented diet.
(3) Overall acceptability was numerically increased with increasing levels of fermented diet in raw and cooked pork loin.
4. The effect of fermented diet on growth performance and blood metabolites of Hanwoo calve are as follows；
(1) Final weight increased with increasing levels of fermented diet.
(2) Average daily gain and feed efficiency were highest in 2% fermented diet (HP) treatment.
(3) Blood urea nitrogen increased with increasing levels of fermented diet.
5. The effect of fermented diet on growth performance, blood metabolites and meat quality of Hanwoo steer are as follows；
(1) Gain and average daily gain were increased with increasing levels of fermented feed additives.
(2) In final blood metabolites, Growth hormone, total cholesterol and blood urea nitrogen were highest in HP treatment.
(3) Aroma and marbling score were higher than 0.5% fermented diet (LP) and 1% fermented diet (MP) treatment compared to CON treatment of raw Longissimus muscle.
(4) Oleic acid (C1 :ln-9) concentration was highest in 1% fermented diet (MP) treatment.