초록 ▼

■ 미래창조과학부의 해외생물소재 확보 및 활용 사업은 국제공동연구사업의 연구 기초가 되는 핵심기반 과학기술국제화 사업으로서, 해외생물 소재의 원천 자원 확보를 목적하는 중요한 사업임
■ 본 연구는 해외생물소재 확보 및 활용 방안을 제시하기 위한 목적으로 아래에 제시된 연구 목표 및 방법으로 수행함
○ 1차년도에는 한국생명공학연구원에서 제공한 해외생물자원 소재의 생물활성 및 약리・효능 데이터를 참고하여 평가된 해외생물자원 소재의 생물활성 검정 결과들을 활용하여 과산화수소를 유발시킨 신경세포주 치매모델에서 해외생물자원 소재의

■ 미래창조과학부의 해외생물소재 확보 및 활용 사업은 국제공동연구사업의 연구 기초가 되는 핵심기반 과학기술국제화 사업으로서, 해외생물 소재의 원천 자원 확보를 목적하는 중요한 사업임
■ 본 연구는 해외생물소재 확보 및 활용 방안을 제시하기 위한 목적으로 아래에 제시된 연구 목표 및 방법으로 수행함
○ 1차년도에는 한국생명공학연구원에서 제공한 해외생물자원 소재의 생물활성 및 약리・효능 데이터를 참고하여 평가된 해외생물자원 소재의 생물활성 검정 결과들을 활용하여 과산화수소를 유발시킨 신경세포주 치매모델에서 해외생물자원 소재의 신경세포 보호, 치매 예방 및 치료효과를 위한 약리・효능 평가를 완료함
○ 2차년도에는 선정된 해외생물소재에서 in vitro 실험을 이용하여 추출물/분획물에서 치매예방 및 치료효과 평가를 완료함
○ 3차년도에는 해외생물소재의 활성성분 분리/분석을 완료하여 in vivo 동물모델에서의 치매예방 및 치료효과를 수행함
○ 4차년도에는 1, 2차년도의 수행된 연구결과를 바탕으로 해외생물소재 추출물/분획물들의 안전성을 평가하고 기준 및 규격 표준화 확립함
○ 5차년도에는 해외생물소재의 in vivo early PK를 평가하고 최적화된 추출물을 개발함

Abstract ▼

Results
○ In 1^st year, we have performed the selection of candidated extracts of foreign biological resources, as well as evaluated the neuroprotective and/or therapeutic effects of extracts/fractions of foreign biological resources in vitro cellular models
• We have assessed the ne

Results
○ In 1^st year, we have performed the selection of candidated extracts of foreign biological resources, as well as evaluated the neuroprotective and/or therapeutic effects of extracts/fractions of foreign biological resources in vitro cellular models
• We have assessed the neuroprotective effect of 2,675 kinds of foreign biological resources on oxidative stress-induced neuronal cell death based on the database of 12,000 kinds of foreign biological resources by KRIBB using in vitro cellular models

○ In 2^nd year, we have evaluated the therapeutic and/or pharmacological effects of extracts/fractions of foreign biological resources in vitro cellular models
• We have selected 70 foreign biological resources which has the neuroprotection activity over 90% out of 2,675 kinds of foreign biological resources
• We have selected 3 foreign biological resources which the bulk samples can be supplied for pre-clinical trial study

○ In 3^rd year, we have completed the separation/analysis of the active components of foreign biological resources, as well as carried out the neuroprotective and/or therapeutic effects of it’s components using in vivo mice models
• We have determined the effects of extracts of Portulaca grandiflora Hook. and Vaccinium bracteatum Thunb. on neuroprotection and therapeutic activities, as well as, it’s target mechanism studies using in vivo mice models
• We have completed the two domestic/PCT patent applications using foreign biological resources of Portulaca grandiflora Hook. and Vaccinium bracteatum Thunb.

○ In 4^th year, we have assessed safety of extracts/fractions of foreign biological resources carried out on the basis of the results of previous years, and we have established standardized criteria and standards of the extracts
• We have also confirmed the bulk supply agreement of Portulaca grandiflora Hook. and Vaccinium bracteatum Thunb. for a stable supply of materials
• We have identified the seven major components of Portulaca grandiflora Hook. and two major components of Vaccinium bracteatum Thunb.

○ In 5^th year, we have evaluated the early PK of foreign biological resources and have studied the development of optimized extract
• We have evaluated the early PK of Vaccinium bracteatum Thunb., and isoorientin, it’s components
• We have demonstrated the effects of isoorientin on neuroprotection, anti-dementia, and/or prevention/therapeutic activities using in vitro cellular and in vivo mice models
• We have found two novel components and five major components of Portulaca grandiflora Hook. and have developed the optimized extract/fractions.

○ Finally, we have completed candidated sources to developed food or treatment for neurodegenerative diseases including cognitive dysfunction and dementia from foreign biological resources