보고서 정보
주관연구기관 |
충북대학교 산학협력단 Chungbuk National University |
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 |
한국어
|
발행년월 | 2015-02 |
과제시작연도 |
2014 |
주관부처 |
농촌진흥청 Rural Development Administration(RDA) |
등록번호 |
TRKO201600010868 |
과제고유번호 |
1395038533 |
사업명 |
지역전략작목산학연협력사업(보조,광특+제주계정) |
DB 구축일자 |
2016-11-05
|
DOI |
https://doi.org/10.23000/TRKO201600010868 |
초록
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Ⅳ. 연구개발결과
제 1세부과제 : 환경휴면기 중의 복숭아 품종별 내한성 구명
탈순화 전·후의 품종별 내한성과 저온요구도 측정, 탈순화 처리에 따른 발아시기 조사 결과 복숭아에서 탈순화 전의 내한성이 탈순화기의 개화시기와 관련이 있음을 확인하였으며 ‘대월’, ‘미스홍’, ‘미홍’, ‘아이카와나카지마’, ‘이즈미백도’가 탈순화 저항성이 강하며 저온요구도가 긴 품종들로 확인되어 최근 동해 피해에 적응할 수 있는 품종들로 선발하였다.
환경휴면기 중의 내한성 변화와 관련하여 탄수화물과 proline 함량 분석 결과
Ⅳ. 연구개발결과
제 1세부과제 : 환경휴면기 중의 복숭아 품종별 내한성 구명
탈순화 전·후의 품종별 내한성과 저온요구도 측정, 탈순화 처리에 따른 발아시기 조사 결과 복숭아에서 탈순화 전의 내한성이 탈순화기의 개화시기와 관련이 있음을 확인하였으며 ‘대월’, ‘미스홍’, ‘미홍’, ‘아이카와나카지마’, ‘이즈미백도’가 탈순화 저항성이 강하며 저온요구도가 긴 품종들로 확인되어 최근 동해 피해에 적응할 수 있는 품종들로 선발하였다.
환경휴면기 중의 내한성 변화와 관련하여 탄수화물과 proline 함량 분석 결과 10품종 모두 탈순화기에 glucose와 fructose의 함량이 감소하였으며 sorbitol 함량 또한 대부분의 품종에서 감소하였다. 지속적인 탈순화 처리에 따른 복숭아 10품종의 proline 함량은 탈순화 처리 후에 증가하는 경향을 보였다. Proline 생합성과 관련된 P5CS, P5CR 유전자의 발현은 P5CS는 대부분의 품종에서 탈순화 처리 후에 감소하였고, P5CR은 증가하였다.
제 1협동과제 : 노린재류에 의한 복숭아 피해 경감 기술 개발
썩덩나무노린재와 톱다리개미허리노린재 모두 기주에 약액을 처리했을 때 보다 충체에 약액을 직접 분무했을 때 살충효과가 높았다.
포장실험 결과 Etofenprox가 기피반응을 보였고 Buprofezin+Dinotefuran, Etofenprox+Flubendiamide는 무반응을 보였으며 살충률은 낮게 나타났다.
유인이나 무반응 보다는 Etofenprox와 같이 기피를 나타내는 약제를 이용하면 약제살포 후 유입되는 노린재에 의한 피해를 막을 수 있다.
제 2협동과제 : 이산화염소수 및 열풍처리 방법을 이용한 복숭아 저장성 증진
이산화염소수 처리에 따른 복숭아의 저장 중 품질 특성 검토 결과, 이산화염소수 30ppm을 10∼15분 처리 시 부패율, 경도 및 기호도가 우수하였다. 또한 기존 연구 결과, 3∼6시간 열풍(46℃)처리시 부패율, 무게변화, 저장성, 경도 및 기호도가 우수하여 이산화염소수 처리와 열풍(46℃) 병용처리 후 저장 중 품질 특성 검토 하였다. 그 결과 이산화염소수 단독 또는 병용처리시에는 3시간 내외에 부패율, 경도 및 기호도가 우수하였다.
Abstract
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제 1세부과제 : 환경휴면기 중의 복숭아 품종별 내한성 구명
Recent temperature patterns have become irregular because of global warming. Erratic warm spells in spring followed by freezing temperature can cause freezing injury in deacclimated temperate fruit trees. Thus, recent required cultivars should be not only ver
제 1세부과제 : 환경휴면기 중의 복숭아 품종별 내한성 구명
Recent temperature patterns have become irregular because of global warming. Erratic warm spells in spring followed by freezing temperature can cause freezing injury in deacclimated temperate fruit trees. Thus, recent required cultivars should be not only very hardy to extremely low temperatures, but also adaptable to unexpected changes of temperature or capable of avoiding erratic temperature patterns through deeper and longer dormancy. This study was conducted to investigate differences in cold hardiness and floral bud opening of 10 peach cultivars (‘Aikawanakajima’, ‘Daewol’, ‘Izumi Hakuto’, ‘Janghowon Hwangdo’, ‘Kiraranokiwami’, ‘Mihong’, ‘Misshong’, ‘Soomee’, ‘Suhong’, and ‘Sun Gold’) during an experimental deacclimation. Furthermore, soluble carbohydrates, proline (Pro) and expression of related genes (P5CS and P5CR) were analyzed during the experimental deacclimation.
The percent injury data based on electrolyte leakage analysis under various temperature conditions were transformed into LT50 values representing cold hardiness. Branch cuttings from 10 cultivars were placed in a greenhouse to force floral bud break. Chilling requirement was measured using Chill days model. Carbohydrates were analyzed using a HPLC. Pro was analyzed using the ninhydrin method and its related gene expressions were examined using quantitative real-time RT-PCR.
Floral bud opening was correlated with cold hardiness before the deacclimation treatment. Deacclimation-tolerance in response to constant warm temperature did not parallel with cold hardiness. During deacclimation, glucose, fructose and sorbitol contents decreased. While cold hardiness of 10 peach cultivars decreased during deacclimation, Pro conent of those increased. Notably, at the same time, expression of P5CS was positively correlated with cold hardiness declined in 10 peach cultivars, whereas expression of P5CR was negatively correlated with cold hardiness.
제 1협동과제 : 노린재류에 의한 복숭아 피해 경감 기술 개발
Peach is economically important fruit, which is next to many cultivation of apples, persimmon, citrus, and grape in Korea. Hemipteran is one of the strong mobility pests in peach. They go away to the other fileds or weeds when the insecticide sprayed. So, it is difficult to control given the damage by moving to the field again. Black-brown spots on the fruit that will decrease the quality of the products are appeared by sucking of Hemipteran, Therefore, development of reduction technique for damaging is necessary. If we have a insecticide that is no-response, non-repellent or attractive effect during spray treatment, there are believed to be effective for controlling. This study was performed using the insecticides for their effects.
We investigated the forecasting occurrence and development characteristics of Hemipteran in peach orchards. Adults of Riptortus clavatus and Halyomorpha halys were examined behavioral responses to host plants for repellency, attractant and unresponsive insecticides selection. The experiment was performed using insecticides that is registered with hemipteran in orchard. Behavioral responses were examined as a mortality of selected insecticides for the adults and nymphs in semi-field.
Both Riptortus clavatus and Halyomorpha halys were higher insecticidal activity when sprayed directly on the insect body than host plant.
The results of field experiment, Etofenprox showed repellent effect and Buprofezin+Dinotefuran did not respond. However, mortalities of both insecticides was very low.
If you use repellent pesticide such as Etofenprox will be able to prevent the damage of a Hemipteran that is better than attraction or no-response.
These results are to be effective for hemipteran control and will be provided as the basic study for development of hemipteran behavior management agents.
Damage reduction technique by using behavioral analysis from Hemipteran is expected to
제 2협동과제 : 이산화염소수 및 열풍처리 방법을 이용한 복숭아 저장성 증진
To prolong the shelf-life of domestic peach, samples were treated with aqueous chlorine dioxide(ClO2) of 30 ppm for 0~15 minute and the spoilage rate, changes in physico-chemical and sensory properties of treated samples were investigated. The control showed spoilage at day 4, and then 31.25% of control showed spoilage at day 8. However, samples treated with aqueous ClO2 were not spoilage at day 4. And 6.25% of samples treated with aqueous chlorine dioxide for 5 min showed spoilage at day 6. After 8 days, spoilage rate of aqueous ClO2 treated for 10 and 15 min were not spoilage. Weight change of control was higher than that of aqueous ClO2 treated samples for 8 days storage. There were no difference in pH of samples among treatments but they were increased by storage time. There were no difference in firmness of samples among treatments but firmness of control sample was decreased faster than those of the aqueous ClO2 treated samples for 8 days storage. No significant changes in lightness, redness and yellowness of the samples by aqueous ClO2 treatment were observed during storage. The sensory parameters including taste, flavor, color, texture and overall acceptance at initial period were not different among treatments. The scores for taste, flavor, texture and overall acceptance of control were decreased faster than those of aqueous ClO2 treated samples during storage.
To improve the shelf-life of the domestic peach, samples were treated aqueous chlorine dioxide(ClO2) of 30 ppm for 10 minute and then treated with 46℃ hot air for 0~9 hours. And the spoilage rate, changes in physico-chemical and sensory properties of treated samples were investigated. The spoilage rate of control was 25.00% at day 4, and then 45.31% of control showed spoilage at day 6. However, the spoilage rate of samples treated with aqueous ClO2 were showed 11.25% at day 4 and aqueous ClO2-hot air combination treatment were showed 12.50, 12.50 and 13.75% at day 4, respectively. The spoilage rate of control was higher than those of aqueous ClO2 or combination treatment. Weight change was not different among the control and aqueous ClO2 or combination treatment and gradually increased during the storage period in all treatment. No significant changes in lightness, redness and yellowness of the samples by control, aqueous ClO2 or combination treatment were observed during storage. There were no difference in firmness of samples among treatments, but firmness of the control sample was decreased faster than those of the aqueous ClO2 or 3 hour hot air combination treated samples at day 4. And then firmness of all treatments were rapidly decreased after day 4. The sensory parameters including taste, flavor, color, texture and overall acceptance at initial period were not different among treatments. But the scores for texture and overall acceptance of control were decreased faster than those of aqueous ClO2 and 3 hour hot air combination treated samples at day 2. And then sensory parameters of all treatments were decreased after day 4.
목차 Contents
- 표지 ... 1
- 제 출 문 ... 2
- 요 약 문 ... 3
- S U M M A R Y ... 7
- 목차 ... 10
- 제 1 장 서 론 ... 11
- 제 2 장 국내외 기술개발 현황 ... 13
- 제 3 장 연구개발수행 내용 및 결과 ... 15
- 제 4 장 연구개발목표 달성도 및 대외기여도 ... 51
- 1절 목표대비 달성도 ... 51
- 2절 정량적 성과 ... 52
- 제 5 장 연구개발결과의 활용계획 ... 55
- 제 6 장 연구개발과정에서 수집한 해외과학기술정보 ... 55
- 제 7 장 기타 중요 변동사항 ... 56
- 제 8 장 국가과학기술종합정보시스템에 등록한 연구장비 현황 ... 56
- 제 9 장 참고문헌 ... 57
- 끝페이지 ... 64
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