초록

온열 치료는 소규모의 후향적 연구는 있으나, 아직 임상적인 근거를 인정받지 못하고 있어 급여 항복이 되지 못하고 있으나, 본 연구를 통하여 온열 치료와 방사선의 병합치료 시, 항종양 효과가 있은 것으로 관찰되어, 자궁경부암 환자 중 일차 동시 화학방사선 요법을 받는 FIGO stage 2B 이상의 환자들에게 온열 치료를 병합하는 임상시험을 제안 할 수 있는 근거가 마련되었다고 할 수 있다.

(출처 : 보고서 요약서)

Abstract ▼

Purpose : To investigate the synergistic effect of radiation with oncothermia in xenograft mouse model using cervical cancer cells
Methods : We made a xenograft mouse model with 5wk balb/c nude mouse. For tumor growth delay experiment, we divided the mice into 4 group; radiation, oncothermia, com

Purpose : To investigate the synergistic effect of radiation with oncothermia in xenograft mouse model using cervical cancer cells
Methods : We made a xenograft mouse model with 5wk balb/c nude mouse. For tumor growth delay experiment, we divided the mice into 4 group; radiation, oncothermia, combined, control. We chose five weeks old nude mice to transplant ME-180 cells into right flank. Oncothermia was given when tumor mass grew up to 7~8mm as mean diameter. Radiation was given under anesthesia up to 9 Gy. The size of mass was measured with caliper with two or three days interval.
Results : We calculated tumor growth delay with the interval between mass volume of 150mm3 and 900mm3. Until the day #33, 1) mass grew up to 1480mm3 in control, 2) 1104mm3 in radiation group, 3) 1232mm3 in oncothermia group, 4) 902mm3 in combination group. The time interval between tumor mass volume of 150mm3 and 900mm3 were as follows ; 1) 21 days in control, 2) 28 days in radiation group, 3) 24 days in oncothermia group, 4) 33 days in combination group. Therefore tumor growth delay was measured as 7 days in radiation group, 3 days in oncothermia group, and 12 days in combination group.
Conclusion : The combination group showed better response compared to radiation alone or oncothermia alone. We are going to propose a phase three multi-institutional randomized controlled trial for locally advanced cervical cancer patients through Korea Gynecologic Oncology Group.

(출처 : SUMMARY)

목차 Contents

• 표지 ... 1제 출 문 ... 2보고서 요약서 ... 3요 약 문 ... 4SUMMARY ... 5CONTENTS ... 6목차 ... 7제1장 연구배경과 필요성 ... 8제2장 국내외 기술개발 현황 ... 10제3장 연구개발수행 내용 및 결과 ... 11 1) 실험동물 및 실험군 편성 ... 11 2) 종양 성장 지연 분석 ... 11 3) 폐전이 양상 관찰 ... 12 4) 면역화학염색 ... 12 5) RT-PCR ... 12 6) TUNEL assay and SA-β-gal staining ... 13제4장 목표달성도 및 관련분야에의 기여도 ... 13제5장 연구개발결과의 활용계획 ... 13제6장 참고문헌 ... 13끝페이지 ... 14