초록

● 환자의 대장 종양 조직을 이용하여 human tumor organoid의 제작이 가능함을 전향적 임상실험을 통해 입증함.
● 환자 유래 organoid의 banking이 가능하도록 freezing, thawing 기술 및 adenovirus를 이용한 유전자도입 기술을 획득함.
● 쥐 소장 organoid를 이용하여 방사선장염시 줄기세포보호를 통해 회복을 빠르게 할 수 있음을 증명함.

(출처 : 보고서 요약서)

Abstract ▼

Ⅳ. Results
65 patients with colonic neoplasm (adenoma and adenocarcinoma) were enrolled prospectively under the approval of IRB. Paired tissues of normal and neoplastic lesion were procuredthrough the biopsy or endoscopic resection during colonoscopic evaluation.
After measuring weight of tiss

Ⅳ. Results
65 patients with colonic neoplasm (adenoma and adenocarcinoma) were enrolled prospectively under the approval of IRB. Paired tissues of normal and neoplastic lesion were procuredthrough the biopsy or endoscopic resection during colonoscopic evaluation.
After measuring weight of tissue, crypts were isolated by EDTA chelation, washing with keratinocyte basal medium gold, suspended in Matrigel and grown in culture media containing EGF, noggin, R-spondin 1, and Wnt-3a. We measured the success rate of tumor organoids, duration of viable organoid, and proliferation rate using EdU staining, and then analyzed this data with several clinical parameters. Of the 49 patients’ specimens (till Sep 2016), success rate of paired organoids was 81.63% (40/49), success rate of passage was 95% (38/40). Success rate of first half was 70.8% (7/24), but its second half was 92% (2/25). Causes of failure were the inadequate culture media (5/9), small amount of isolated crypts (2/9), and bacterial contamination (2/9). The required amount of tissue was at least 0.07g, equivalent to twice biopsies. There was no correlation between success rate of tumor organoids and clinical factors such as age, sex, body mass index, location of tumor, pathology of tumor, level of mutated p53, and ki-67 index.

(출처 : SUMMARY)

목차 Contents

• 표지 ... 1제 출 문 ... 2보고서 요약서 ... 3요 약 문 ... 4SUMMARY ... 6CONTENTS ... 8목차 ... 9제 1 장 연구개발과제의 개요 ... 10제 2 장 국내외 기술개발 현황 ... 14제 3 장 연구개발수행 내용 및 결과 ... 15 제 1 절 이론적 접근방법 ... 15 제 2 절 실험적 접근방법 ... 15 제 3 절. 연구내용 및 결과 ... 19제 4 장 목표달성도 및 관련분야에의 기여도 ... 24제 5 장 연구개발결과의 활용계획 ... 25제 6 장 연구개발과정에서 수집한 해외과학기술정보 ... 26제 7 장 참고문헌 ... 27끝페이지 ... 29