Abstract ▼

As part of Korean Epigenome Study, we need to produce a large sample size of epigenetic data and analyze epigenome-wide association to identify chronic disease markers. For this purpose, we performed Illumina MethylationEPIC BeadChip scanning of a 400 Korean cohort.

To get DNA resources from

As part of Korean Epigenome Study, we need to produce a large sample size of epigenetic data and analyze epigenome-wide association to identify chronic disease markers. For this purpose, we performed Illumina MethylationEPIC BeadChip scanning of a 400 Korean cohort.

To get DNA resources from Korea Biobank Network, the study plan was reviewed by institutional review board, and 400 DNAs were transferred. Then DNAs were bisulfite-converted using Zymo Research's EZ Methylation^TM Kit, and the conversion efficiency was checked by using sanger sequencing traces.

Illumina's MethylationEPIC BeadChip (850K) was used for epigenome data production. All 400 DNAs fulfill detection P-value < 0.01 in 99% of markers, and sample call rate > 98%. Raw signal intensities were quantile normalized, and then we performed beta mixture quantile dilation using BMIQ, and background correction using ENmix. The adjusted beta-values were used for further analyze; eg. principal component analysis and epigenome-wide association study. In addition, we validated 10 CpG sites of 400 DNAs using EpiTyper^TM platform.

Finally, we delivered raw idat data and Beta-value to department of management.

In this study, we produce Korean epigenome data, and this data will be used for the discovery of epigenetic marker for chronic disease. Large cohort epigenome data is essential for development of disease marker and treatment method.
Therefore this project will be helpful for chronic disease prognosis and diagnosis.
Also it will contribute to improving the quality of life.

( 출처 : SUMMARY )

목차 Contents

• 표지 ... 1
• 학술연구개발용역과제 연구결과점검보고서 ... 2
• 목차 ... 3
• 연구결과점검보고서 요약문 ... 4
• Summary ... 5
• 학술연구개발용역과제 연구결과 ... 6
• 제1장 최종 목표 ... 6
• 제2장 국내외 기술 현황 ... 7
• 제3장 최종 연구 내용 및 방법 ... 8
• 제4장 최종 연구 결과 ... 15
• 제5장 연구결과 고찰 및 결론 ... 41
• 제6장 연구성과 및 활용계획 ... 42
• 제7장 연구용역과제 진행과정에서 수집한 해외과학기술정보 ... 44
• 제8장 기타 중요변경사항 ... 44
• 제9장 연구비 사용 내역(당해년도) 및 연구원 분담표 ... 44
• 제10장 참고문헌 ... 45
• 제11장 첨부서류 ... 46
• 끝페이지 ... 66