초록 ▼

○ 본 연구는 물렁증원인체 동원핵편모충(Neobodo 목)의 생활사 구명 및 멍게 양식방법 개선으로 멍게 양식생산량 증대를 위하여 수행되었다.

○ 건강종묘 및 조기종묘생산을 위해 어미관리시 필요한 적정먹이, 먹이효율, 먹이공급횟수 등을 조사하여 멍게양식 어업인에게 기술 보급하였다.

○ 외해어장 개발을 위해 경남 통영시 욕지면해역에 연구교습어장을 승인받아 건강종묘 및 덩이식 채묘를 시험양식하였으며, 그 결과 종묘의 생존율은 40∼80%를 나타내었으며, 물렁증 발생은 기존어장(술역)과 외해어장에서 모두 관찰되지

○ 본 연구는 물렁증원인체 동원핵편모충(Neobodo 목)의 생활사 구명 및 멍게 양식방법 개선으로 멍게 양식생산량 증대를 위하여 수행되었다.

○ 건강종묘 및 조기종묘생산을 위해 어미관리시 필요한 적정먹이, 먹이효율, 먹이공급횟수 등을 조사하여 멍게양식 어업인에게 기술 보급하였다.

○ 외해어장 개발을 위해 경남 통영시 욕지면해역에 연구교습어장을 승인받아 건강종묘 및 덩이식 채묘를 시험양식하였으며, 그 결과 종묘의 생존율은 40∼80%를 나타내었으며, 물렁증 발생은 기존어장(술역)과 외해어장에서 모두 관찰되지 않았다.

○ 조기종묘는 2014년 10월 6일 부터 12월 1일까지 생식소 인위성숙 조절을 통하여 100연을 채묘하였으며, 통영시 추원해역에 가이식하여 관리하였다.

○ 물렁증원인체인 동원핵편모충의 생활사를 파악하기 위하여 동원핵편모충의 계절적 이동 경로 추적, 탈낭유발요인 및 동원핵편모충의 진단용 특이항체를 개발하여 생활사를 분석하였다.

○ 동원핵편모충의 계절적 감염조사 결과 오직 척삭동물에서만 감염이 나타나 피낭류가 숙주인 것으로 조사되었다.

○ 국내 남해안 멍게 양식장에서는 봄철 물렁증이 발생한 후, 여름철이 되어 수온이 상승하면 물렁증이 소멸하는 특성을 나타내고 다시 가을철 수온이 내려가면서 서서히 발생하는 패턴을 보인다. 여름철 물렁증 소멸은 물렁증에 감염된 개체는 모두 죽고, 고수온으로 인해 A. hoyaumushi의 증식이 일어나지 않으므로 물렁증 감염이 숙주인 피낭류에 잠복되어 있는 것으로 추정되었다

(출처:보고서 요약 p.3)

Abstract ▼

Ⅵ. Results of R&D

1. Improvement of culturing method

○ Development of technology for production of healthy seedlings
- Seedlings were collected after the management of the mother for 2 weeks through indoor cultivation.

○ Development of technology for early stage production of

Ⅵ. Results of R&D

1. Improvement of culturing method

○ Development of technology for production of healthy seedlings
- Seedlings were collected after the management of the mother for 2 weeks through indoor cultivation.

○ Development of technology for early stage production of seedlings
- For the early stage production of seedlings, 100 strings of seedlings were collected through the artificial maturation control of the gonad over the period of October 6 ~ December 1, 2014,
- The produced seeds were transplanted at the Chuwon area in Tongyeong city.

○ Development of clumping type of culture method
- Seedling collection was onto the oyster shells
- 500 strings of the seeds were collected.

○ Development of open sea culturing technology
- Research and training fishing farm (2 locations)
• Area : 20 ha at the Sangnodae waters in Yokji-myeon, Tongyeong City, Gyeongnam
• Area :10 ha at the Cheongsa waters in Yokji-myeon, Tongyeong City, Gyeongnam
- The survival rate during the cultivation was 100% with no observation of the occurrence of soft tunic syndrome.

2. Investigation of the life cycle and development of the treatment agent for the pathogenic agent of soft tunic syndrome, A. hoyamusi (Neobodo )

○ Development of technology for the optimization of diagnosis of A. hoyamusi within sea squirt aquaculture farms
- For the survey on the occurrence rate of A. hoyamushi, all organisms thriving in the areas around the sea squirt culture farm were collected in May, August and November for the analysis of the presence of infection by A. hoyamushi. The infection rate by A. hoyamushi was low in the summer and infection was observed in the chordates regardless of the season.

○ Investigation of the life cycle of A. hoyamusi
- For the purpose of investigation of the life cycle of the pathogenic agent for soft tunic syndrome. A. hoyamusi, has been pure cultured over a period of 7 days. As the results of the culturing, circular shaped cysts were observed. Cyst was generated under the hypoxic condition of 5mg/l (DO).

○ Study on the applying the treatment agent for the tunic-softness syndrome onto the culture farm sites
- For the purpose of seeking the environment-friendly treatment agent for soft tunic syndrome. The killing power of 8 types of organic acids on A. hoyamushi was conducted by in vitro test.
- As the results of the test, it is deemed that the treating of the soft tunic syndrome by using organic acid would not be possible due to the lack of the ability of organic acid to penetrate through the tunic of the sea squirts.

3. Investigation of the infection route and life cycle of the pathogenic agent for the soft tunic syndrome

○ Investigation of the life cycle through the study on the factors that induce excystation of A. hoyamusi
- Cyst of A. hoyamusi is divided into suspended and adhesive cysts.
- The conditions of excystation of the suspended cysts were not confirmed. However, the adhesive cysts, flagella are generated again and conversion cysts into flagellate when the pH is lowered.

○ Development of specific antibody for the diagnosis of A. hoyamusi
- A. hoyamusi specific antibody was developed in order to examine whether it exists in the form of A. hoyamusi or cyst within the live body of the sea squirt.

○ Investigation of the life cycle of pathogenic agent of soft tunic syndrome through the tracking of the infection route of A. hoyamusi with the changes in seasons
- Infection was observed only in chordates among the benthic organisms.

○ Investigation of the mechanism of the occurrence of the soft tunic syndrome
- The sea squirts aquaculture farms in the South Sea in Korea display occurrence of soft tunic syndrome in the spring with the extinction of the soft tunic syndrome with the increase in the water temperature in the summer. However, as the water temperature drops in the autumn, the manifestation of the soft tunic syndrome increases again.
- The extinction of soft tunic syndrome in the summer is presumed to be the results of the perishing of the sea squirts infected with soft tunic syndrome and the latency of the infection of soft tunic syndrome in the cysts, which is the host, due to the failure of the proliferation of A. hoyamusi to occur due to high water temperature.
- Recurrence of the soft tunic syndrome in the autumn that follows with the drop in water temperature is due to the mass proliferation of A. hoyamusi remaining in the sea squirts and other Ascidian in the lower portions of the culture farm as the water temperature drops. It is presumed that this proliferated A. hoyamusi then infects animal with degraded physiological immunity, thereby generating mass occurrence of tunic-softness syndrome

(출처:SUMMARY p.11)

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 보고서 요약 ... 3
• 요약문 ... 4
• SUMMARY ... 9
• 목차 ... 15
• CONTENTS ... 17
• 그림목차 ... 19
• FIGURE LISTS ... 21
• 표목차 ... 23
• TABLE LISTS ... 24
• 제 1 장 연구개발과제의 개요 ... 25
• 제 2 장 국내외 기술개발 현황 ... 26
• 제 3 장 연구개발수행 내용 및 결과 ... 27
• 제 1절 연구개발 수행 방법 ... 27
• 1. 양식방법 개선 연구 ... 27
• 2. 물렁증 원인체 동원핵편모충(Neobodo 목)의 이동경로 및 생활사 특성 구명(위탁과제) ... 28
• 제 2절 연구개발 수행 내용 및 결과 ... 32
• 1. 양식방법 개선 연구 ... 32
• 2. 물렁증원인체 동원핵편모충(Neobodo 목)의 생활사 구명 및 치료제 개발 ... 41
• 3. 멍게 물렁증원인체 이동 경로 및 생활사 특성 구명 ... 45
• 제 4 장 목표달성도 및 관련분야에의 기여도 ... 54
• 제 5 장 연구개발결과의 활용계획 ... 54
• 제 6 장 참고문헌 ... 55
• 끝페이지 ... 56