[보고서]방사선 복합 생물반응 연구

김진홍

[국가R&D연구보고서] 방사선 복합 생물반응 연구
Biological Responses to Combined Action of Radiation 원문보기

보고서 정보
주관연구기관	한국원자력연구원 Korea Atomic Energy Research Institute
연구책임자	김진홍
참여연구자	김진규 , 김동호 , 노창현 , 정동민 , 강미영 , 류태호 , 장은영
보고서유형	최종보고서
발행국가	대한민국
언어	한국어
발행년월	2014-12
과제시작연도	2014
주관부처	미래창조과학부 Ministry of Science, ICT and Future Planning
연구관리전문기관	한국원자력연구원 Korea Atomic Energy Research Institute
등록번호	TRKO201700007574
과제고유번호	1711017452
사업명	한국원자력연구원연구운영비지원
DB 구축일자	2017-10-12
키워드	이온화 방사선.복합작용.생물방호.세포반응.복합작용 세포신호.p53.방사선방어.커큐민.ionizing radiation.biodosimetry.combined effects radiation protection.cell signals.p53.radiomodification.curcumin.
DOI	https://doi.org/10.23000/TRKO201700007574

초록 ▼

방사선 연구개발의 국가 연구주체인 한국원자력연구원 첨단방사선연구소는 인체를 포함한 방사선 생물방호의 근간이 되는 방사선 생물선량 평가 및 반응검출 기술개발의 임무를 가지고 있다. 원자력 사고 또는 방사선 이용의 증가에 따른 대비책으로 방사선 생물방호 원천기술의 개발과 기술도약을 이루기 위한 노력을 기울여왔으며 특히, 방사선을 과학, 의학, 산업 분야 등에 이용함에 있어 생물학적 효용성을 증대시키기 위한 기술근거로서 방사선과 물리 • 화학적 요인의 복합작용 심화연구를 수행하여 국제적 기술우위를 확보하였다. 방사선과 다양한 물리·화학적 요인의 복합 생물반응을 검출하고 이를 응용할 수 있는 기술을 개발함으로써 국내 방사선 분야 기술우위와 국격 향상에 기여함과 동시에 국제사회의 방사선 연구 패러다임 변화와 생물방호 신개념에 부합하는 생물학적 선량평가 및 생물방호 원천기술을 확립하기 위한 연구를 수행하였다. 생물방호 및 생물선량 평가 분야에서 국제적으로 인정받는 방사선생물학 연구실로 발돋움하는데 기여하고 방사선의 과학적 이용 효율을 증대시키는 데 필요한 기술근거를 제공할 것이다.

(출처: 서지정보양식 초록 p.124)

Abstract ▼

The responsibility of KAERI ARTI is to research and develop radiation technology. Strong motivation and RT-based biotechnologies necessary for the future R&D plans can be provided from the results of this research. An advanced analytical method was suggested for the combined action of ionizing radiation with another factor. Using the method, it is possible to predict in advance the maximum value of synergistic interaction and the conditions to achieve the maximum. By elaborating the present research results, new technologies will be established for analysing the combined effects of radiation with another factor and radiosensitivity of organisms. Biodosimetry techniques implemented in this research can play an important role for ARTI to be an internationally recognized radiation biology and biodosimetry laboratory. The results of this study give a clues for establishment of important technology associated with enhancing positive efficacy of radiation applications.

(출처:BIBLIOGRAPHIC INFORMATION SHEET Abstract p.125)

목차 Contents

표지 ... 1
제출문 ... 2
요약문 ... 3
SUMMARY ... 10
CONTENTS ... 17
목차 ... 19
표목차 ... 20
그림목차 ... 21
제1장 서론 ... 24
제2장 본론 ... 28
제1절 방사선유발 염색체 이상 (dicentrics)을 이용한 생물선량 평가 ... 30
제2절 분자지표 (γ-H2AX)를 이용한 분자선량 평가 기반연구 ... 41
제3절 방사선조사에 따른 세포신호전달 및 세포사멸 과정 분석연구 ... 51
제4절 방사선 노출에 따른 세포생존율 측정 검증 ... 67
제5절 커큐민의 방사선 방어효과 ... 75
제6절 세포에 대한 고 LET 및 저 LET 방사선의 복합작용 ... 88
제3장 결론 및 건의사항 ... 102
제4장 참고문헌 ... 106
서지정보양식 ... 124
BIBLIOGRAPHIC INFORMATION SHEET ... 125
끝페이지 ... 125

표/그림 (27)

표 Results of chromosomal aberrations following in vitro irradiation of human peripheral blood from five donors
표 Pooled data of chromosomal aberrations after in vitro irradiation of human peripheral blood from five donors
표 Yield and intercellular distribution of dicentric chromosomes after in vitro irradiation of human peripheral blood from five donors
표 Dose-response curve for chromosomal aberrations (dicentric chromosomes and centric ring chromosomes) induced by gamma rays in lymphocytes derived from five donors. The fitted values of the coefficients of the linear quadratic function y = c + αD + βD2: c = 0.0149 ± 0.0611; α = 0.2165 ± 0.0574; β = 0.0274 ± 0.0110. Error bars represent the standard error.
표 Dose-response curve for dicentric chromosomal aberrations induced by gamma rays in lymphocytes derived from five donors. The fitted values of the coefficients of the linear quadratic function y = c + αD + βD2: c = 0.0123 ± 0.0578; α = 0.2106 ± 0.0544; β = 0.0249 ± 0.0104. Error bars represent the standard error. Dotted lines are the upper and lower 95% confidence intervals. Error bars represent the standard error.
표 The representative pictures of metaphase lymphocytes after irradiated with 0 - 5 Gy gamma rays at 1,000 magnification. Solid arrows are dicentric chromosomes and dotted arrows are acentric fragments.
표 Repregentative image of MCF-7 cells irradiated with 10 Gy gamma-rays at 1,000 fold magnification: (A) Untreated control, (B) γ-H2AX foci formation at 2 h post-irradiation. Relative H2AX phosphorylation intensity was calculated by ImagePartner 4.0 software.
표 γ-H2AX foci formation in MCF-7 cell after 1 Gy gamma-rays irradiation: (A) 0, (B) 15 min, (C) 1 h, (D) 2 h and (E) 24 h after irradiation.
표 The plot for the linear dynamic range of γ-H2AX foci per cell following irradiation with 1 Gy gamma rays.
표 γ-H2AX evaluation in MCF-7 cell at 15 min post-irradiation: (A) 0, (B) 1 Gy, (C) 5 Gy and (D) 10 Gy.
표 Dose-dependence of γ-H2AX foci formation in MCF-7 cell at 15 min post-irradiation.
표 p53 activates p21 but not proapoptotic targets at 24 h post-irradiation. Western blot analysis of HeLa cells. HeLa cells were isolated and analyzed for the expression of proteins related to cell cycle arrest and apoptosis at the indicated incubation time. β-actin was used as a loading control.
$The induction of p21 by p53 influences cell cycle distribution. (A) Repregentitive histograms analyzed by flow cytometry showing G1 arrest 24h post treatment and apoptotic sub G1 fraction at 72h. (B) Cell cycle distribution at 24h post-irradiation. (C) Percentage of apoptosis in HeLa cells 72h post treatment. Any sub-G1 populations were counted as apoptotic cells.$ 표 The induction of p21 by p53 influences cell cycle distribution. (A) Repregentitive histograms analyzed by flow cytometry showing G1 arrest 24h post treatment and apoptotic sub G1 fraction at 72h. (B) Cell cycle distribution at 24h post-irradiation. (C) Percentage of apoptosis in HeLa cells 72h post treatment. Any sub-G1 populations were counted as apoptotic cells.
표 HeLa cells show no evidence of p53-dependent cellular senescence. Images of Senescence Associated β-galactosidase staining of HeLa cells.
표 Low levels of p53 trigger autophagy. (A) Detection of radiation-induced appearance of autophagic organelles by vital staining with lysosomotropic agents, acridine orange. (B and C) Effects of inhibitors for autophagy, 3-MA, on acridine orange positive (+) cells and the conversion of LC3-I to LC3-II, respectively.
표 High levels of p53 trigger apoptosis. (A) Caspase-3 activity as measured by caspase-3 colorimetric assay kit at the indicated time incubation. Z-VAD-fmk ompletely blocked caspase-3 activity at 72h post-irradiation. (B) Apoptosis (%) was abrogated by z-VAD-fmk. (C) Cleaved PARP expression induced by apoptotic cell death was reduced by z-VAD-fmk treatment.
표 Radiation-induced cell viability on HepG2 cell by using Trypan blue assay (A) and MTT assay (B). Cells were plated on 96 well plates (1 x 104cells/well) for 1 day and then irradiated with 0– 100 Gy. After incubation for 1, 2 and 3 days, cell viability was measured by both assays, respectively. Data are mean±SD of triplicate determinations.
표 Radiation-induced cell survival on HepG2 cell. Cells were plated on 96 well plates (1 x 104 cells/well) for 1 day and then irradiated with 0– 100Gy. After incubation for 1, 2 and 3 days, the Clonogneic assay was carried out. Data are mean±SD of triplicate determinations.
표 Parameters of DNA damage induced by IR in HepG2 cells
표 Chemical structure of curcumin.
표 Cytotoxicity of curcumin by using MTT assay. Data are represented as mean ± SD (n=3). (n=3). *p < 0.05, **p < 0.01 compared with control group.
표 Effect of curcumin against IR-induced cytotoxicity in HepG2 cells as determined by the Clonogenic survival assay. Data are represented as mean ± SD . (n=3). *p < 0.05, **p < 0.01 compared with control group.
표 Comet images of the IR alone and curcumin plus IR group in HepG2 cells.
표 Effect of curcumin against IR-induced genotoxicity in HepG2 cells as measured by the Comet assay. HepG2 cells irradiated with 5 Gy. Data are represented as mean ± SD . (n=3). *p < 0.05, **p < 0.01 compared with control group.
표 Survival curves ((A)-(C), left panel) obtained immediately after γ-irradiation (closed circles) and after complete post-irradiation recovery (open circles); cell survival((A)-(C), right panel); (A).the first irradiation; (B).the second exposure; (C).the third exposure. Experiments were performed with diploid S. cerevisiae yeast cells, strainXS800.
표 As in Fig. 6-1, but after exposure to α-particles.
표 The parameters characterizing the process of radiation recovery

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[국가R&D연구보고서] 방사선 복합 생물반응 연구
Biological Responses to Combined Action of Radiation 원문보기