Abstract ▼

□ Purpose
Many researchers want to use plant hydrolysates for fermentative purposes since they are a renewable source of sugars. However, hydrolysis of lignin results in numerous phenolic acids being produced, which are toxic to the bacteria. The purpose of this study is to answer both of these i

□ Purpose
Many researchers want to use plant hydrolysates for fermentative purposes since they are a renewable source of sugars. However, hydrolysis of lignin results in numerous phenolic acids being produced, which are toxic to the bacteria. The purpose of this study is to answer both of these issues so that this study will use toxicogenomics and microfluidics to develop novel reactors and study the effects of the hydrolysate compounds on several bacterial strains, respectively. Furthermore, genetically-modified strains will be constructed to provide enhanced butanol yields and productivities (>20 g/L).

□ Contents
This study involves several projects with a common goal – imiproved usage of hydrolysates for higher butanol yields and productivities.
○ Enhancement of butanol production utilizing biomass
• Toxicogenomic study of bacterial strains (e.g. E. coli and C. beijerinckii) exposed to lignin-hydrolysates and development of hydrolysate-tolerant strains with fermentation system for high-yield butanol production
• Hydrolysate compound mineralization with natural isolates and its optimization, and high-yield butanol production within toxic enviroment
○ Microfluidic study for optimization of fermentation processes
• Development and characterization of a novel light-inducible gene expression method
• Development of microfluidics platform for Toxicogenomic study and cell patterning techniques for optimal butanol production parameters
• Design of novel micro-reactor systems and its application

□ Results
• Devleopment of quantitative methods using bioluminescent strains to detect hydrolysate compounds
• Toxicogenomic study of E. coli and C. beijerinckii exposed to ferulic acid and identification of responsive functional gene sets. Develpment of tolerant strain based on this study
• Design of In-situ recovery fermentation systme and optimization and obtain of 25 g/L of butanol
• Development of microfluidic screening device and its application to toxicogenomics
• Desgin of light switchable gene expression system and mL scale microbioreactor
• Minimization of uL scale bioreactor for adpatation and HTS application
• Optimization of microbial metabolite using microfluidic devices

□ Future plan
novel strains and optimized parameters from microfluidics developed for enhanced biofuel production will be expanded and applied to commercial fermentation processes. Also, the novel fermentative strains developed by mutated and recombinated for hyper-butanol producing can be provided and utilized in domestic/international parts. This interdisciplinary study will contribute to development of bio-energy technology.
(출처:SUMMARY 5p)

목차 Contents

• 표지 ... 1제출문 ... 2보고서 요약서 ... 3요약문 ... 4SUMMARY ... 5CONTENTS ... 6목차 ... 7제1장 연구개발과제의 개요 ... 8 제1절 연구개발의 필요성 ... 8 제2절 연구개발의 목표 및 범위 ... 8제2장 국내외 기술개발 현황 ... 10 제1절 세계적 수준 ... 10 제2절 국내 수준 ... 10 제3절 국내외 연구현황 및 전망 ... 10제3장 연구개발수행 내용 및 결과 ... 12 제1절 연구범위 및 연구수행 방법 ... 12 제2절 연구 내용 및 연구 결과 ... 15제4장 목표달성도 및 관련 분야에의 기여도 ... 96 제1절 세부과제의 목표 달성도 ... 96 제2절 평가의 착안점에 따른 관련 분야에의 기여도 ... 99제5장 연구개발성과의 활용계획 ... 103 제1절 연구결과의 활용방안 ... 103 제2절 기대성과 ... 104제6장 연구개발과정에서 수집한 해외 과학기술 정보 ... 105제7장 연구시설 및 장비현황 ... 106제8장 참고 문헌 ... 107끝페이지 ... 110