초록 ▼

▷ 한국인 집단에서 다양성이 높은 국산 Multiplex STR 시스템 및 miniSTR multiplex system(10-plex miniSTR system) 개발하여 유용성을 검토하였음.
▷ 고감도로 human genomic DNA를 검출하기 위하여 Rubpy 형광체 도핑 실리카 나노입자를 역 마이크로에멀젼 시스템 방법을 이용, 합성하여 배수희석시킨 genomic DNA에 biotinylated Alu probe를 붙이고 Rubpy 형광체 도핑 실리카 나노입자를 반응시킨 후 Rubpy 형광체 도핑 실리카 나노입자의 형광을

▷ 한국인 집단에서 다양성이 높은 국산 Multiplex STR 시스템 및 miniSTR multiplex system(10-plex miniSTR system) 개발하여 유용성을 검토하였음.
▷ 고감도로 human genomic DNA를 검출하기 위하여 Rubpy 형광체 도핑 실리카 나노입자를 역 마이크로에멀젼 시스템 방법을 이용, 합성하여 배수희석시킨 genomic DNA에 biotinylated Alu probe를 붙이고 Rubpy 형광체 도핑 실리카 나노입자를 반응시킨 후 Rubpy 형광체 도핑 실리카 나노입자의 형광을 측정해 본 결과 R²= 0.9879인 calibration curve를 얻었고 검출한계는 0.1ng/ul 였음.
▷ Affymetrix SNP array 데이터를 토대로 Y 염색체 SNP 마커 996개, X 염색체 SNP 마커 37607개를 데이터 베이스화 했으며, 미토콘드리아 SNP 마커 783개를 확보하였고, 개인식별에 최적인 상염색체 마커 50개, X, Y염색체 마커 각각 15개, 미토콘드리아 마커 16종을 선정하고, 이를 동시검출 가능한 방법을 고안, 도출된 결과들이 개인식별에 이용 가능함을 확인.

( 출처 : 요약서 3p )

Abstract ▼

• Interspersed short tandem repeat (STR) loci consist of tandem arrays ranging from 3 to 5 base pairs in length, which represent a significant fraction of eukaryote genomes.
The STRs have features of polymorphic markers useful for the gene mapping of the human genome, disease diagnosis, and perso

• Interspersed short tandem repeat (STR) loci consist of tandem arrays ranging from 3 to 5 base pairs in length, which represent a significant fraction of eukaryote genomes.
The STRs have features of polymorphic markers useful for the gene mapping of the human genome, disease diagnosis, and personal identification in the medical and forensic sciences. International standardization system construction in forensic science through improvement of Domestic production of DNA analysis Kit in association with and commercial markers.

• In the forensic community, due to the short PCR amplicons (<145 bp), mini STR systems can effectively be used in forensic analysis with highly degraded DNAs.
Currently, miniSTR markers can be identified by fluorescence-based automatic multiplex systems using commercial products by goods imported (e.g., ABI Co., USA).
However, these systems are not only expensive, but also not so suitable in the Korean population samples. Thus, We were developed the 10-plex miniSTR multiplex system using 9 CODIS markers (D18S51, D8S1179, FGA, D21S11, vWA, D16S539,D3S1358, THO1, TPOX) with amelogenin. We were also evaluated the 10-plex miniSTR multiplex system to degraded DNA and can provide improved signal from degraded DNA than commercial kit (PowerPlex 16, Promega, USA). The Exact Test demonstrated that all loci surveyed here were found to be no deviation from Hardy-Weinberg equilibrium. The combined probability of match calculated from 9 miniSTR loci was 1.123x10-10, which is high degree of polymorphism. Thus, this 10-plex miniSTR system may be suitable for recovering useful information in analyzing degraded DNA samples from Korean population and it will provide economic advantage in the field of forensic study.

• Identification and quantification of human genomic DNA isolated from a spot of blood is performed before multiplex short tandem repeats (STRs) PCR is performed. Highly sensitive detection of human genomic DNA is an important issue because the amount of human genomic DNA acquried from a spot of blood is limited and the genomic DNA is apt to be easily degraded. The present study suggests a powerful method to surpass detection sensitivity of conventional human genomic DNA quantitation based on Alu probes and nanoparticles such as quantum dot. The silica nanoparticles fabricated by reverse microemulsion capsulate a large number of converntional dyes and reduce oxidation of conventional dyes. A variety of surface chemistry has been developed to bind biological functional groups to the surface of silica nanoparticles.
Based on the conjugation chemistry, silica nanoparticle can be conjugated to the Alu probes that specifically bind to human genomic DNA. This conjugation also contributes to the enhancement of detection sensitivity in the human genomic DNA. Gold nanoparticle is a promising material to provide another enhancement of detection sensitivity in the human genomic DNA. Particularly, gold nanoparticle has high affinity to proteins. This arises from hydrophobic, electrostatic interaction and dative bonding interaction between proteins and gold nanoparticles.

• Human identification technology first began in 1985 when DNA profiling and DNA fingerprinting was discovered. Since then, this technology has gradually improved to the current widespread method of analyzing STR (Short Tandem Repeat) markers. Despite the high accuracy and advanced analysis methods that are available today for human identification, confirming familial relationships is still impeded by the possibility of false positives due to the high rate of genetic mutations.

• In order to overcome such obstacles, new analysis techniques that utilize SNP (single nucleotide polymorphism) information have emerged. The SNP markers were selected from database based on their MAF (minor allele frequency), which were around 0.4 to 0.5. We have completed development of SNP gentotyping tools which consists of 96 markers for SNPs that are ideal for human identification.

( 출처 : SUMMARY 8p )

목차 Contents

• 표지 ... 1제 출 문 ... 2보고서 요약서 ... 3요 약 문 ... 4SUMMARY ... 8CONTENTS ... 10목차 ... 11제1장 연구개발과제의 개요 ... 12제2장 국내외 기술개발 현황 ... 14제3장 연구개발수행 내용 및 결과 ... 18 제1절 : STR 분석 기술 및 국산 키트 개발 ... 18 제2절 : 실리카 나노입자 및 Alu probe에 기초한 고감도 Human genomic DNA 검출 ... 35 제3절 : 개인식별 및 혈연관계 예측용 SNP 마커 다중 판별 tool 개발 ... 41제4장 목표달성도 및 관련분야에의 기여도 ... 58제5장 연구개발결과의 활용계획 ... 65제6장 연구개발과정에서 수집한 해외과학기술정보 ... 68제7장 연구시설ㆍ장비 현황 ... 75제8장 참고문헌 ... 76끝페이지 ... 79