Abstract ▼

Purpose&contents
Ultimate Aim of the study
Augmentation of the proliferation and differentiation potentials of human bone marrow mesenchymal stem cells in vitro by using nicotinamide

Specific Aims
1. Confirmation of the potentiatial effect of NAM on BMSC proliferation and differentia

Purpose&contents
Ultimate Aim of the study
Augmentation of the proliferation and differentiation potentials of human bone marrow mesenchymal stem cells in vitro by using nicotinamide

Specific Aims
1. Confirmation of the potentiatial effect of NAM on BMSC proliferation and differentiation
2. Elucidation of the underlying mechanisms of the effects of NAM
3. Animal study for the efficacy evaluation of NAM-treated stem cell delivery in vivo and the effect of NAM on tissues

Research contents
1. Confirmation of the effect of NAM on BMSC proliferation extension.
2. Identifying the mechanisms underlying BMSC proliferation extension by NAM.
3. Analysis of the effect of NAM on BMSC differentiation (osteogenesis; adipogenesis; chondrogenesis; osteogenic to adipogenic shift upon cellular aging)
4. Identifying the changes in the expression of the factors involved in BMSC differentiation
5. Identifying the effect of NAM on BMSC osteogenesis efficacy in vivo
6. Identifying the effect of NAM on differentiated cell viability and functionality

Result
1. Confirmation of the positive effects of NAM on BMSC replicative lifespan and growth rate
① Confirmed NAM-induced attenuation of the proliferation decline in continued BMSC cultivation
② Confirmed NAM-induced attenuation of senescence imposition in aged BMSC

2. Confirmation of the association of mitochondria quality improvement and SIRT1 activation to the positive effects of NAM on BMSC proliferation extension

3. Confirmation of the positive effects of NAM on BMSC differentiation
① Observed the sustained differentiation of osteogenesis, adipogenesis, chondrogenesis in late passage BMSC upon NAM treatment
② Observed the sustained expression of the transcription factors and marker genes of osteogenesis and chondrogenesis in late passage BMSCs upon NAM treatment

4. Confirmation of the positive effect of NAM on osteogenesis of BMSC in vivo
① Confirmed osteogenic differentiation in mouse transplanted with NAM-treated BMSC without an osteogenic stimulation
② Waiting to observe the sustained osteogenesis in vivo of NAM-treated late passage BMSC

5. Confirmed the positive effect of NAM treatment on the viability of neurons
① Observed an increase in the viability of mouse embryonic neurons and mitochondria quality maintenance

Expected Contribution
1. Development of a new cell cultivation condition that allows MSC replicative life span extension for the use in cell therapies.
2. Development of a new safe technique that allows high efficacy osteogenesis.
3. Practical improvement in cell therapy for poor chondrogenic conditions.
4. A new pharmacological protocol for prevention and treatment of poor chondrogenic conditions
5. Development of a new supplement or protocol in MSC culture media and conditions

(출처 : SUMMARY 6p)

목차 Contents

• 표지 ... 1목차 ... 3연구계획 요약문 ... 4연구결과 요약문 ... 5 한글요약문 ... 5 SUMMARY ... 6연구내용 및 결과 ... 7 1. 연구개발과제의 개요 ... 7 2. 국내외 기술개발 현황 ... 7 3. 연구수행 내용 및 결과 ... 10 4. 목표달성도 및 관련분야에의 기여도 ... 19 5. 연구결과의 활용계획 ... 20 6. 연구과정에서 수집한 해외 과학기술정보 ... 20 7. 주관연구책임자 대표적 연구실적 ... 21 8. 참고문헌 ... 21 9. 연구성과 ... 24별첨 ... 26끝페이지 ... 32