초록 ▼

1. 자궁내막증, 흡연, 자궁 근종 등이 여성 난임에 미치는 영향을 임상적으로 분석하고, 난포, 난자 및 배아 발달의 과정에 미치는 영향을 실험을 통해 증명함.
2. 난소기능 평가의 기준 및 그 적응증을 제시함.
3. 체외수정 결과를 예측하는 모델을 제시하고, 체외수정 결과를 증진시킬 수 있는 세포질내정자주입술의 기준, 난소자극 프로토콜, 배아이식 가이드라인을 제시함.
4. 체외수정 결과에 영향을 미치는 요인을 수용체, 자가항체, mRNA 등의 기초 단계에서 분석함.
5. 정자의 선별 기준을 제시하고, 정자 선

1. 자궁내막증, 흡연, 자궁 근종 등이 여성 난임에 미치는 영향을 임상적으로 분석하고, 난포, 난자 및 배아 발달의 과정에 미치는 영향을 실험을 통해 증명함.
2. 난소기능 평가의 기준 및 그 적응증을 제시함.
3. 체외수정 결과를 예측하는 모델을 제시하고, 체외수정 결과를 증진시킬 수 있는 세포질내정자주입술의 기준, 난소자극 프로토콜, 배아이식 가이드라인을 제시함.
4. 체외수정 결과에 영향을 미치는 요인을 수용체, 자가항체, mRNA 등의 기초 단계에서 분석함.
5. 정자의 선별 기준을 제시하고, 정자 선별법이 정자에 미치는 영향을 분석하고, 정자의 유전학적 조절에 관여하는 기전을 밝힘.
6. 배아의 초기 발생과 착상 과정에 대한 효과적인 연구를 위한 실시간 모니터링 시스템과 체외착상 outgrowth 연구 모델을 확립함.
7. Blastocyst와 outgrowth 배아에서 발현이 증가하여 착상 과정에 중요한 역할을 할 수 있는 microRNAs의 동정 및 기능에 대한 연구를 진행함.
8. 배아의 발생을 촉진하고 착상 실패를 극복할 수 있는 해수유래 미네랄을 첨가한 기능성 배양액을 개발함.

(출처 : 보고서 요약서 3p)

Abstract ▼

Purpose& Contents
Basic and clinical skills development to overcome infertiliy.
Intracytoplasmic sperm injection, improvement of environmental factor for IVF, analysis of chromosomomal factors about implantation, histological abnormality of endometrium, loss of implantation ability.
1. To

Purpose& Contents
Basic and clinical skills development to overcome infertiliy.
Intracytoplasmic sperm injection, improvement of environmental factor for IVF, analysis of chromosomomal factors about implantation, histological abnormality of endometrium, loss of implantation ability.
1. To investigate the effects of various characteristics and diseases of women on the outcome of in vitro fertilization
(1) Analyze the effect of endometriosis on the outcome of in vitro fertilization
(2) Establishment criteria for evaluation of ovarian function
(3) Development of effective in vitro fertilization technology for immature oocytes
(4) Analysis of the outcome of in vitro fertilization (IVF) according to the low fertility disease or degree of response to ovarian stimulation, and related technology development
(5) Development of effective in vitro fertilization technology for smoking couple
(6) Effects of autoantibodies on the outcome of in vitro fertilization
2. Search for the genetic mechanism of male infertility and improvement of fertilization technology
(1) Acetylation and methylation of sperm, and sperm microRNA expression on the outcome of in vitro fertilization
(2) Improvement of sperm quality index, fertilization and development rate of embryo by sperm pre-treatment or sperm selection
3. Investigation for the roles of embryos and endometrium during implantation process, and identification of regulating factors for preimplantation development and implantation to improve the implantation rate in human IVF-ET program
(1) Characterization of the embryo trophic factors (ETFs) and implantation essential factors (IEFs)
(2) Identification of differential expressed microRNAs in blastocysts and outgrowth embryos
(3) Development of functional culture media to promote embryonic development and to overcome implantation failure

Results
1. development of effective in vitro fertilization technique
(1) Developing criteria for the determination of diagnosis and treatment policy by analyzing the effect of endometriosis on the ovarian function
(2) Experimental evidence of the effects of endometriosis on follicle growth, fertilization rate and embryo development rate
(3) Exploring therapeutic drugs for women with uterine leiomyoma
(4) Presenting a model that can predict the outcome of in vitro fertilization
(5) Establishing criteria for ovarian function test and establishing new criteria for low-grade ovarian response after induction of ovarian hyperstimulation
(6) Presentation of ovarian stimulation protocol, indications for intracytoplasmic sperm injection, and guidelines for embryo transfer for improvement of fertility and embryo quality in low-response group or low-quality oocyte group
(7) Prove factors affecting embryonic development in receptor, mRNA level
(8) Effects of smoking on the results of assisted reproductive technology
(9) Relationship between thyroid autoantibodies and in vitro fertilization outcomes
(10) Analysis of microenvironmental changes in follicular fluid in women with endometriosis
2. Search for the genetic mechanism of male infertility and improvement of fertilization technology
(1) Establishment of the foundation for the evaluation of semen quality in male cancer patients
(2) The effect of sperm DNA fragmentation on the in vitro fertilization
(2) Analysis of methylation, oxidation and DNA fragmentation before and after swim-up treatment
(3) Comparing DNA fragmentation and diploid, after the swim-up and cook gradient methods
(4) Mechanism to control sperm genes and chromosomes through methylation, acetylation, and Histone acetylation
3. Identification of regulating factors for preimplantation development and implantation to improve the implantation rate in human IVF-ET program
(1) Establishment of in vitro culture system and outgrowth in vitro implantation model using mouse embryos
(2) Identification of embryo trophic factors (ETFs) and implantation essential factors (IEFs) in mouse blastocysts and outgrowth embryos
(3) Efficacy of culture media supplemented with minerals from sea water to increase the implantation rate
(4) Functions of differential expressed microRNAs from outgrowth embryos in implantation process

Expected Contribution
1. In the future infertility research, the effects of endometriosis on fertility are demonstrated through basic research and animal experiments, and based on the results, the basis of research on the treatment of patients with endometriosis is presented.
2. From the technical point of view, we will expand the clinical application by developing a technology that can predict the low-response group and improve the pregnancy rate according to each individual patient.
3. Economically and industrially, our results can reduce the huge personal and social costs of infertility treatment.
4. To present a basic research model for the infertility research of male infertility field and to develop the technology which can be clinically applied in the future.
5. Domestic production of embryo culture and transfer media, which are dependent on foreign technology and acquiring of patent technology, can achieve technical independence, improve international competitiveness, and bring about possibility of foreign currency acquisition through export of medical technology
6. Identification of microRNAs related to embryo development and implantation process can be applied to pathophysiology, prevention and treatment for various women’s diseases in reproductive organs

(출처 : SUMMARY 7p)

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 보고서 요약서 ... 3
• 국문요약문 ... 5
• SUMMARY ... 7
• Contents ... 10
• 목차 ... 11
• 1. 연구개발과제의 개요 ... 12
• 1-1. 연구개발 목적 ... 12
• 1-2. 연구개발의 필요성 ... 12
• 1-3. 연구개발 범위 ... 13
• 2. 국내외 기술개발 현황 ... 14
• 3. 연구수행 내용 및 결과 ... 18
• 4. 목표달성도 및 관련분야 기여도 ... 75
• 4-1. 목표달성도 ... 75
• 4-2. 관련분야 기여도 ... 78
• 5. 연구결과의 활용계획 ... 79
• 6. 연구과정에서 수집한 해외과학기술정보 ... 82
• 7. 연구개발성과의 보안등급 ... 82
• 8. 국가과학기술종합정보시스템에 등록한 연구시설·장비 현황 ... 82
• 9. 연구개발과제 수행에 따른 연구실 등의 안전조치 이행실적 ... 83
• 10. 연구개발과제의 대표적 연구실적 ... 84
• 11. 기타사항 ... 85
• 12. 참고문헌 ... 86
• 별첨 ... 89
• 끝페이지 ... 156