[보고서]방사선융합 유전질환 맞춤형 식ㆍ의약 식물자원 개발

강시용

방사선융합 유전질환 맞춤형 식ㆍ의약 식물자원 개발
Development of plant genetic resources for food and medicinal uses using radiation fusion technology 원문보기

보고서 정보
주관연구기관	한국원자력연구원 Korea Atomic Energy Research Institute
연구책임자	강시용
보고서유형	최종보고서
발행국가	대한민국
언어	한국어
발행년월	2017-03
과제시작연도	2016
주관부처	미래창조과학부 Ministry of Science, ICT and Future Planning
등록번호	TRKO201800004094
과제고유번호	1711035692
사업명	방사선기술개발사업
DB 구축일자	2018-04-28
키워드	방사선융합.돌연변이.분자마커.이차대사산물.식물유전자원.Radiation fusion.Mutant.Molecular marker.Secondary metabolite.Plant genetic resource.
DOI	https://doi.org/10.23000/TRKO201800004094

초록 ▼

○ 연구 목적: 국민 식생활 패턴 변화로 인한 성인병(심혈관계 질병, 당뇨병, 고혈압, 비만) 예방을 위해 방사선융합육종기술을 활용한 차세대 맞춤형 기능성 식ㆍ의약 식물 유전자원 개발 및 산업화

○ 연구개발성과:
1. 유용 식·의약 식물자원 유효성분 및 변이 유전자원 pool 구축
· 유효 기능성 물질 정량 및 정성분석 검정을 통한 기능성 평가기술 개발
· 다양한 방사선원(이온빔, 우주선) 활용 유효성분 변이 및 유전체 연구용 유전자원 pool 확보
· 돌연변이 유전자원 선발과 형질조사에 의한 DB 구축 기반 마련

2. 유용 식·의약 식물자원 선발을 위한 MAS 시스템 구축
· 선발 기능성 계통의 유전체 분석을 통한 타겟 유전자 변이 탐색
· 조기 선발용 마커 개발을 위한 돌연변이계통의 염기변화 특성 분석
· 고 기능성 계통 선발을 위한 대량집단 성분 분석체계 구축

3. 타겟 이차대사산물 합성 경로 규명 및 유전자 네트워크 연구
· 이차대사산물 변이 계통 이용 유전체 구조 변이 비교 연구
· 타겟 이차대사산물 합성 관련 유전자 및 발현조절기작의 규명
· 이차대사산물 변이의 타겟 질환 대상 생체 영향 연구

4. 유전질환 예방 신 유전자원 개발
· 항노화, 항염증 기능성 차조기(약용들깨) 신품종 2종 육성
· 항노화 기능성 벼 신품종 3종 육성과 가공적성 검정
· 비린내 저감 기능성 콩 신품종 육성

○ 연구개발성과의 활용계획:
· 유용 식ㆍ의약 돌연변이 식물 유전자원 개발과 이를 활용한 유전체 연구 및 돌연변이 유전자원은행 구축
· 식생활 패턴 변화 맞춤형 기능성 식ㆍ의약 식물자원 개발과 유전질환 예방을 위한 방사선 식물자원 유래 약리 활성 증진 기능성 소재 개발 및 활용으로 세계시장 경쟁력 확보
· 맞춤형 기능성 식ㆍ의약 식물자원 개발로 국민 건강 증진 및 국가적 의료비 절약 효과 기대

( 출처 : 보고서 요약서 3p )

Abstract ▼

Purpose&Contents
The purpose of this study is to develop plant genetic resources for food and medicinal uses using radiation fusion technology to prevent adult diseases such as cardiovascular diseases, diabetes, hypertension, and obesity.

Results
1. Development of mutation pool of functional food and medicinal plants
· Development of evaluation techniques for functional materials through quantitative and qualitative analysis
· Development of mutation pool using various radiation (ion-beam and space environments)
· Collection of trait data of mutant genetic resources for database construction

2. Development of marker-assisted selection (MAS) system for mutant genetic resources
· Genomic approaches to target gene change in selected mutants
· Analysis of genome change patterns for early MAS system development
· Development of high-throughput screening techniques for high-functional secondary metabolites

3. Analysis of secondary metabolite biosynthesis and gene network
· Study of comparison and structural genomics associated with secondary metabolite biosynthesis
· Analysis of gene expression and regulation associated with secondary metabolite biosynthesis
· Study of medicinal values for target secondary metabolites

4. Development of new medicinal plant resources for genetic diseases prevention
· Development of Perilla cultivars for anti-aging and anti-inflammation
· Development of new rice cultivars for anti-aging and comparison rice-cake quailities
· Development of new soybean cultivar for reduced beany flavor

Expected Contribution
· Medicinal mutant plants will be used for genetic resources in molecular genetic studies and genomic analysis in the breeding program and for important databases in mutant genebank.
· Development of functional food and medicinal plants will provide great profit to the agricultural industry and guarantee intellectual property right.
· Development of functional food and medicinal plants will improve and maintain health and lower health care costs.

( 출처 : SUMMARY 5p )

목차 Contents

표지 ... 1
제 출 문 ... 2
보고서 요약서 ... 3
요약문 ... 4
SUMMARY ... 5
CONTENTS ... 6
목차 ... 7
제 1장. 연구개발과제의 개요 ... 8
1절. 연구개발의 목적 ... 8
2절. 연구개발의 필요성 ... 8
3절. 연구개발 범위 ... 10
제 2장. 국내외 기술개발 현황 ... 11
1절. 국내 기술개발 현황 ... 11
2절. 국외 기술개발 현황 ... 13
제 3장. 연구수행 내용 및 결과 ... 15
1절. 연구개발수행 내용 ... 15
2절. 연구개발수행 결과 ... 33
제 4장. 목표달성도 및 관련분야 기여도 ... 114
제 5장. 연구결과의 활용계획 ... 118
제 6장. 연구과정에서 수집한 해외과학기술정보 ... 120
제 7장. 연구개발결과의 보안등급 ... 128
제 8장. 국가과학기술종합정보시스템에 등록한 연구시설·장비 현황 ... 128
제 9장. 연구개발과제 수행에 따른 연구실 등의 안전조치 이행실적 ... 128
제 10장. 연구개발과제의 대표적 연구실적 ... 129
제 11장. 기타사항 ... 129
제 12장. 참고문헌 ... 130
끝페이지 ... 131

표/그림 (167)

표 Groups of dietary feeding.
표 Whole genome resequencing and comparative analysis of rice cv.
표 Pedigree of the sequenced soybean samples.
표 Schematic representation of the soybean resequencing analysis pipeline.
표 CGH data analysis obtained from rice dwarf mutants.
표 Primer and probe sequences used for semi-quantitative and real-time RT-PCR.
표 Primer sequences used for sequencing of soybean stachyose synthase.
표 Measurement of the oligosaccharide content in soybean mutant lines.
표 Soluble carbohydrates in soybean mutant lines.
표 Phytate biosynthetic pathways in plants and colorimetric assay with quarter seed for selecting high/low phytate lines.
표 Screening of the oil contents in soybean seeds.
표 Fatty acid profile of selected 15 lines by GC-MS analysis.
표 NIR profile of oil content in 510 Gwangan mutant lines.
표 Workflow for soybean high-throughput protein screening and virtual gel image of protein variations in 12 soybean landraces using Caliper Labchip.
표 The number of soybean lines with present/absent seven seed storage protein bands.
표 Seed coat color of soybean mutant lines induced by gamma irradiation.
표 Comparison of anthocyanin contents in seed coats of soybean mutant lines.
표 The contents of glycosides (A) and aglycones (B) in soybean mutant lines.
표 13. ROS scavenging activity in soybean mutant lines using DPPH (A) and FRAP (B).
표 Radical scavenging activities of soybean mutant lines with altered seed coat color.
표 The difference in Trypsin inhibitor activity between Tia and Tib.
표 Changes in body-weights (A) and pancreas-weight (B).
표 Changes in blood glucose concentration in rats of normal control (group I, gray) and experimental groups (black).
표 Change in insulin iummunoreactivity in β cells of pancreatic islets in association with soybean feeding and streptozotocin after 6 weeks.
표 Growth comparison between wild type (Dongan) and 30 Gy ion beam irradiated lines
표 Growth comparison between wild type (Dongan) and 40 Gy ion beam irradiated lines
표 Growth comparison between wild type (Ilpum) and 30 Gy ion beam irradiated lines
표 Growth comparison between wild type (Ilpum) and 40 Gy ion beam irradiated lines
표 Investigation of unique phenotypes in the ion beam irradiated M4 population from Dongan and Ilpum
표 The rice seeds loaded in Russian IBMP BioRisk.
표 Seedling growth of rice loaded in Russian IBMP BioRisk.
표 Seedling growth of rice loaded in BION-M.
표 Growth comparison between wild type (S2) and M2 lines irradiated with 300 Gy gamma rays.
표 Growth comparison between wild type (S6) and M2 lines irradiated with 300 Gy gamma rays.
표 Growth comparison between wild type (S8) and M2 lines irradiated with 300 Gy gamma rays.
표 Unique phenotypes found in rice irradiated lines with 300 Gy gamma rays.
표 Analysis of fatty acids in the seeds of M4 lines derived from cv. Ilmi and cv. Kwangan.
표 Seed fatty acid composition, seed coat color, hilum color and 100 seed weight of M8 seeds derived from cv. Danbaek and cv. Daepung.
표 A part of the selected soybean mutant lines.
표 Traits of the selected soybean lines with brown or black seed coat.
표 A. Seed coat colors and B. Changed pod traits of mutant varieties from cv. Danbaek and cv. Deapung and experimental field in 2016.
표 Qualitative traits and yield component results of seed coat color change varieties from field trial in 2016.
표 Comparison of seed color between DA and T1001-1 mutant lines.
표 Detection of anthocyanin contents in the T1001-1 mutant seeds.
표 Comparison of anthocyanin contents between Dongan and mutant
표 Anthocyanin contents in the T1001-1 mutant seedlings.
표 Brief information of re-sequencing data from Dongan and T1001-1 mutants.
표 MDS plot of Dongan and T1001-1 mutants based on whole genome resequencing results.
표 SNP and InDel polymorphism distribution by chromosomes.
표 Variation blocks of Dongan and T1001-1 mutants based on the Nipponbare reference sequence.
표 Distribution of CNVs on chromosome 4.
표 The list of genes positioning on the CNVs and variant blocks in chromosome 4
표 Expression analysis of five genes related to anthocyanin biosynthesis in rice mutants (DA:Dongan-control).
표 Differential expression of the genes related to anthocyanin biosynthesis pathway based on microarray data.
표 SNPs in the genes related to the anthocyanin biosynthesis pathway between Dongan and the T1001-1C mutant.
표 Primer information for development of SNAP markers related to the anthocyanin biosynthesis pathway in rice.
표 Development of a SNAP marker based on a SNP in the 5' UTR region on the LOC_01g44260 gene.
표 Differentially expressed genes between Dongan and T1001-1 based on microarray data.
표 Functional annotation of up-regulated genes.
표 Functional annotation of down-regulated genes.
표 Module identification of differentially expressed genes using Weighted gene co-expression network analysis
표 Functional annotation of the identified modules.
표 Significantly induced genes related to anthocyanin biosynthesis pathway in T1001-1.
표 Sequence alignment MYB genes of Dongan and T1001-1 mutants.
표 Validation of PCR product size amplified from MYB genes of Dongan and T1001-1 mutants.
표 Summary of soybean genome resequencing.
표 Summary of pre-processing of the resequencing data.
표 Summary of read alignment to the reference genome
표 Summary of the detected SNPs by samples.
표 Summary of the detected InDels by samples.
표 Classification of the detected SNPs based on their genomic position.
표 Classification of the detected InDels based on their genomic position.
표 Polymorphic SNPs between the samples.
표 Polymorphic InDels between samples.
표 Summary of chromosomal distribution of polymorphism between samples.
표 Graphical representation of chromosomal distribution of polymorphism between samples.
표 Summary statistics of designed high resolution melting primers for SNP detection.
표 Examples of designed high resolution melting primers for SNP detection.
표 Genomic distribution of SNP designed high resolution melting primers for SNP detection in BS vs B-25
표 Genomic distribution of SNP designed high resolution melting primers for SNP detection in BS vs PS
표 Genomic distribution of SNP designed high resolution melting primers for SNP detection in B-25 vs PS
표 Summary statistics of designed high resolution melting primers for In/Del detection.
표 Examples of designed high resolution melting primers for In/Del detection.
표 Genomic distribution of In/Del designed high resolution melting primers for SNP detection in BS vs B-25
표 Genomic distribution of In/Del designed high resolution melting primers for SNP detection in BS vs PS
표 Genomic distribution of In/Del designed high resolution melting primers for SNP detection in B-25 vs PS
표 Workflow of CGH analysis in soybean.
표 Detection of CNVs in chromosomal plot and common CNV locations among the three different mutant lines.
표 The number and size of CNVs among the three different mutant lines compared with their original cultivars.
표 Three dwarf mutants selected by gamma-irradiated mutation from cv. Dongan.
표 Distribution of CNVs in the three dwarf mutants.
표 Detection of common CNV locations in three different mutant lines and the putative candidate gene for dwarf phenotype.
표 Phenotype comarison between Dongan (DA) and a mutant MRXII.
표 The accumulation of total tocochromanol and individual tocochromanols in seeds of DA (control) and MRXII.
표 Germination rates of DA and MRXII rice seeds.
표 Tocochromanol biosynthetic pathways in plants.
표 Transcript levels of vitamin E biosynthesis genes in rice plants.
표 Immunoblot analysis of OsVTE2 in MRXII and DA.
표 Comparison of the promoter (A) and genomic DNA (B) sequences from DA and MRXII mutant with the related region of OsVTE2. The nucleotide sequence data are available from RAP-DB under accession number Os06g0658900.
표 Comparison of the promoter sequences from DA and high tocopherol accumulation mutant with the related region of OsVTE3.
표 Development of CAPS marker based on SNP in the promoter region at the position -264 of the OsVTE2 gene.
표 Weighted gene coexpression network analysis (WGCNA) of tocopherol biosynthesis related genes.
표 Germination efficiency and plant growth of WT and MT.
표 Gene expression of DA and MT based on the microarray analysis.
표 Mapping of the modules onto the MT-related gene co-expression network.
표 Metabolism overview of the differentially expressed genes in the MT mutant.
표 Differential expression of genes related to the secondary metabolism in the MT mutant.
표 Expression of genes related to sucrose-starch pathway and jasmonic acid synthesis.
표 Variation of contents between DA and the mutant MR1001.
표 Seed storage protein patterns in soybean mutant lines using by SDS-PAGE.
표 Colorimetric assay among soybean mutant lines lacking lipoxygenase.
표 Comparison of agricultural traits among soybean mutant lines.
표 Dendrogram of the five soybean mutant lines lacking lipoxygenase and original cultivar using 1,563 AFLP polymorphic bands.
표 Gene structure and sequence variation of Lox1 in H70.
표 Gene structure and sequence variation of Lox2 in H70.
표 Gene structure and sequence variation of Lox3 in H70.
표 Mature rice grains of Dongan and mutants Z183 and Z184..
표 Anthocyanin gene expression in Dongan and seed color mutants.
표 Summary statistics of resequencing and mapping of Dongan and seed color mutants.
표 Classification of detected SNPs and Indels in ongan and seed color mutants based on the reference genome (A) and phylogenetic analysis (B).
표 Variation distribution of Dongan, Z183, and Z184 compared to the Nipponbare genome sequence by chromosomes.
표 Variation distribution of Dongan, Z183, and Z184 compared to the Nipponbare genome sequence by chromosomes.
표 Transition and transversion frequencies of the SNPs deteced in Dongan, Z183, and Z184 compared to the Nipponbare genome sequence.
표 Distribution of the substitution types of the SNPs detected in Dongan, Z183, and Z184 compared to the Nipponbare genome sequence.
표 Annotation summary of the variations detected in Dongan, Z183, and Z184.
표 Nonsynonymous SNPs and Indels in Dongan, Z183, and Z184.
표 Distribution of nonsynonymous variations of Dongan, Z183, and Z184 compared to the Nipponbare genome sequence by chromosomes.
표 Distribution of variations causing amino acid changes in F3H (OS09g18450), DFR (OS01g44260), OSB1 (OS04g47080) genes.
표 Comparison of WT and mutant phenotypes.
표 Weighted gene coexpression netwrok analysis (WGCNA) of 1645 DEGs in mutant.
표 Expression patterns of significant DEGs of MT at different developmental stages.
표 Functional classes and gene expression profiling estimated by the MapMan software.
표 Transcript levels of genes involved in the sucrose-starch metabolic pathway.
표 A. Growth habit segregation of P-5-12-11. B. Comparing of seed coat color and lightness of two P-5-12-11 and cv. Paldal.
표 Nucleotide and amino acid sequence change of stachyose synthase gene in selected P-5-12-11.
표 Three nucleotide changes on genic region of STS between Paldal and P-5-12-11 (A). STS protein structure prediction of Paldal and P-5-12-11(B).
표 Phenotypic comparison between Chookyoupjaso and Atom-Ros.
표 Comparison of rosmarinic acid contents between Chookyoupjaso (CJC) and Atom-Ros (12).
표 Inhibition activity comparison of NO production between Chookyoupjaso (CJC) and Atom-Ros (12).
표 Phenotypic comparison between Chookyoupjaso and Atom-Ketone.
표 Comparison of isoegomaketone contents between Chookyoupjaso (CJC) and Atom-Ketone (CJM-45).
표 Inhibition activity comparison of NO production among Chookyoupjaso (CJC), Atom-Ros (12), and Atom-Ketone(45).
표 Phenotypic comparison between Dongan and Tocomi-1ho.
표 Comparison of tocopherol contents between Dongan and Tocomi-1ho
표 Comparison of tocotrienol contents between Dongan and Tocomi-1ho
표 Comparison of tocopherol and tocotrienol contents between Dongan (DA) and Tocomi-1ho (MRXII).
표 Phenotypic comparison between Dongan and Tocomi-2ho.
표 Comparison of tocopherol contents between Dongan and Tocomi-2ho.
표 Comparison of tocopherol and tocotrienol contents between Dongan and Tocomi-2ho.
표 Phenotypic comparison between Tocohongmi and Dongan.
표 Comparison of tocopherol contents between Dongan and Tocohongmi.
표 Comparison of tocopherol contents between Dongan and Tocohongmi.
표 Comparison of anthocyanin contents between Dongan and Tocohongmi.
표 Comparison of anthocyanin contents between Dongan and Tocohongmi.
표 Comparison of amino acid contents among Dongan, Youngan and GoldAmi-2ho
표 Comparison of amino acid contents between Dongan and GoldAmi-2ho.
표 Inherent characteristics of H70 and cv. Hwanggumkong.
표 Phenotypic characteristics of H70 compared to the original cv. Hwanggumkong.
표 Yield and yield components of H70 and cv. Hwanggum on the yield trial in Jeongeup.
표 Seed crude lipid and unsaturated fatty acid content of H70 and cv. Hwanggumkong.
표 Yield of H70 on the regional yield trials carried out at 3 locations.
표 Amino acid contents in seed of new cultivars.
표 Comparison of seed crude protein content of four rice cultivars.
표 Comparison of pectin cotent in brown rice and brown rice cake by cultivar.
표 Comparison of free sugar content of brown rice cake by cultivar.
표 Sensory characteristics of brown rice cakes by cultivar.
표 BIO-RISK에 탑재될 시료와 BIO_RISK 실린더, ISS 우주정거장 및 ISS 우주정거 장에 고정된 BIO-RISK 모습.

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방사선융합 유전질환 맞춤형 식ㆍ의약 식물자원 개발
Development of plant genetic resources for food and medicinal uses using radiation fusion technology 원문보기