초록 ▼

연구목표:
- PAMP 및 DAMP 분자의 선천면역유발, 세포내 신호상호작용, 만성 염증유발 기전을 밝히고자 함,
- 염증으로 인한 세포내 대사와 상호작용 연구과정에서 염증치료를 위한 분자 표적을 발굴/검증하고자 함.

연구추진내용:
- 연구에 약 35명의 연구진이 참여함.
- 연구시너지와 시기 적절성을 위해 공동연구를 장려하였음.
- 각 연구 소주제에 대해 기능적인 작은 연구팀별로 연구하였고 관련성에 따라 단독/공동협력연구를 진행함.

연구성과:
- 학술적 성과 및 특허 기술이

연구목표:
- PAMP 및 DAMP 분자의 선천면역유발, 세포내 신호상호작용, 만성 염증유발 기전을 밝히고자 함,
- 염증으로 인한 세포내 대사와 상호작용 연구과정에서 염증치료를 위한 분자 표적을 발굴/검증하고자 함.

연구추진내용:
- 연구에 약 35명의 연구진이 참여함.
- 연구시너지와 시기 적절성을 위해 공동연구를 장려하였음.
- 각 연구 소주제에 대해 기능적인 작은 연구팀별로 연구하였고 관련성에 따라 단독/공동협력연구를 진행함.

연구성과:
- 학술적 성과 및 특허 기술이전에 대한 관심과 함께 “SCI 논문 22편 (상위 30% 이상 학술지 게제 13건 IF 5이상 은 총 8편); 특허 5건 출원” 성과를 확보함.
- 연구교수/박사후 연구원과 박사/석사 학위생의 유기전 관계속에서 석사 11명 박사 3명 배출함.
- 임상적 질병과의 연계성을 갖도록 내부 및 임상의와의 연구발표 및 세미나 활성화를 추진하여 “선천감염면역 관련한 특성화” 기반을 구축함.

(출처 : 보고서 요약서 3p)

Abstract ▼

Purpose
This study is proposed to understand the role of PAMP and DAMP molecules in innate immunity. We will focus on components of Pseudomonas aeruginosa as PAMP molecules, and HMGB1, peroxiredoxin and CIRP molecules as DAMP molecules. We will study the innate immune responses (inflammation, inf

Purpose
This study is proposed to understand the role of PAMP and DAMP molecules in innate immunity. We will focus on components of Pseudomonas aeruginosa as PAMP molecules, and HMGB1, peroxiredoxin and CIRP molecules as DAMP molecules. We will study the innate immune responses (inflammation, inflammasome, novel receptors for DAMP/PAMP, unidentified functions of DAMPs) and its interaction with metabolic pathway. Throughout our study, we expect to understand the comprehensive network of host-pathogen interaction and therapeutic target molecules.

contents
• 1st year: Screening PAMP/DAMP molecules related to both early immune response and metabolism. (1) the scavenger recepters for DAMP (HMGB1, Peroxiredoxin, CIRP) and their effects, (2) the viperin expression induced by PAMP (pathogenic peudomonas factor)/DAMP and its association with mitochondira. (3) the innate immunity-inducing factors in PAMP molecules, and (4) PAMP molecules which interacts with host-mucosal substances.
• 2nd year: Correlation between PAMP/DAMP-induced intracellular inflammatory signal and metabolism pathway. (1) the role of PAMP/DAMP molecules in intracellular signalling of microbial/bacterial infection, inflammasome, and autophagy, (2) the role of viperin in both metabolic change and pathogenic microbial/bacterial infection, (3) PAMP-specific host factors and their reaction mechanism, and (4) functional antimicrobial agents derived from host mucusal substances
• 3rd year: Immunity induced by PAMP/DAMP molecules. (1) the immune modulatory functions of DAMP in intracellular metabolism, (2) the role of viperin in the activation of immune cells and inhibition against pathogenic microbial infections, (3) the mechanism that the gene expression in mutant pathogen affects the function of dendritic cells in innate immunity, and the inhibitory mechanism in innate immunty of the gene expression of pathogen in mouse infection model.

Developement results
- Viperin expression is involved in the metabolic changes in both immune cells and cancer cells and in the acquisition of anti-cancer immunity.
- Pseudomonas aeroginosa-derived infection factors is able to degrade the components of inflammasome
- P. aeroginosa-derived genes were identified to be essential in airway mucus secretion.
- The identified E. coli -derived specific genes are involved in induction of the intestinal microenvironment allowing to be sensitive against the infection of Vibiro cholera.
- GM-CSF-dependent bone marrow-derived cells are involved in the inhibition of T cell growth and its differentiation.

Expected Contribution
- Systematic functional analysis of immune-regulatory proteins controlling anti-bacterial innate immunity, and elucidation of the roles of early-period innate immunity components in infectious disease prognosis.
- Understanding the correlation between DAMP molecules, innate immunity, and chronic inflammation, thus suggesting DAMP molecules as therapeutic target.
- Considering new links between metabolic pathways and cell-signaling pathways related to infection.
- Develop researchers in the biomedical field concentrating in infection and host defense.

(출처 : SUMMARY 5p)

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 보고서 요약서 ... 3
• 요 약 문 ... 4
• SUMMARY ... 5
• 목차 ... 6
• 1. 연구개발 목표 및 내용 ... 7
• 가. 최종 목표 ... 7
• 나. 단계목표 ... 8
• 다. 당초 목표의 수정․보완(중요 연구변경) 사유 ... 8
• 라. 1단계 연차별 연구목표 및 내용 ... 9
• 2. 연구 추진전략 및 방법 ... 10
• 3. 주요 연구개발 결과 ... 16
• 가. 목표달성도 ... 16
• 나. 대표적 연구업적 ... 38
• 다. 기타 연구성과 ... 38
• 4. 연구수행에 따른 문제점 및 개선방향 ... 40
• 5. 연구개발성과 현황 ... 40
• 6. 국가과학기술종합정보시스템에 등록한 연구시설․장비 현황 ... 57
• 7. 기타사항 ... 57
• 끝페이지 ... 58