초록 ▼

본 연구과제를 통해,
1. 인간배아줄기세포를 이용한 혈관 및 혈관주위전구세포 분화유도 및 세포치료기술 개발함.

2. 인간화 생쥐를 이용한 인간 전분화능줄기세포 및 분화세포의 생체 내 면역원성 검증함.

3. 안전한 리프로그래밍 줄기세포 생산기술과 세포치료기반기술 개발함.

4. 특정 체세포로의 리프로그래밍 기술개발을 통한 역분화 조절기작의 규명과 응용연구를 진행.

5. 혈관내피세포에서 후성유전체 조절인자의 기전 연구 및 줄기세포 유래의 혈관내피세포의 기능 규명 모델 시스템 개

본 연구과제를 통해,
1. 인간배아줄기세포를 이용한 혈관 및 혈관주위전구세포 분화유도 및 세포치료기술 개발함.

2. 인간화 생쥐를 이용한 인간 전분화능줄기세포 및 분화세포의 생체 내 면역원성 검증함.

3. 안전한 리프로그래밍 줄기세포 생산기술과 세포치료기반기술 개발함.

4. 특정 체세포로의 리프로그래밍 기술개발을 통한 역분화 조절기작의 규명과 응용연구를 진행.

5. 혈관내피세포에서 후성유전체 조절인자의 기전 연구 및 줄기세포 유래의 혈관내피세포의 기능 규명 모델 시스템 개발함.

(출처 : 요약서 3p)

Abstract ▼

Purpose & Contents
Research Purpose
Developing clinically applicable human pluripotent stem cells derived specific cell types for treatment of incurable diseases.

Research content
. Cell therapy development using perivascular progenitor cells derived from human embryonic stem cells

Purpose & Contents
Research Purpose
Developing clinically applicable human pluripotent stem cells derived specific cell types for treatment of incurable diseases.

Research content
. Cell therapy development using perivascular progenitor cells derived from human embryonic stem cells
. Assessing immunogenicity of human pluripotent stem cells and their derivatives in humanized mice
. Developing techniques for safe iPSC generation, and their use in cell therapy
. Identification and application of mechanisms underlying direct reprogramming into specific cell types
. Epigenetic mechanisms of endothelial cells and developing appropriate animal models to assess functionality of hESC derived endothelial cells

Results
. Production and cell therapy development of hESCs-PVPCs (3 SCI publications, 1 patent application)
. Assessment of immunogenicity of human pluripotent stem cells and their derivatives in humanized mouse (1 SCI publication and 1 in preparation)
. Safe reprogramming techniques for iPS generation and their differentiation (5 SCI publications and 3 patent applications)
. Studying the role of an epigenetic regulator Dot1L in endothelial cells, and generating appropriate diseased animal models to test functionality of hESC-endothelial cells (knock-out mice)

Expected Contribution
. Findings related to therapeutic efficacy and mechanism, and clinical safety of hESCs-PVPCs are expected to provide a platform to develop safe and effective cell therapies for various types of vascular diseases.

. Fundamental research in human pluripotent stem cells will not only add novel findings to the existing body of knowledge, but also expect to contribute to technological advance in stem cell therapy development.

(출처 : SUMMARY 5p)