초록

• 골분화에 관여하는 microRNA의 작용 및 조절 network를 구명하여 골분화를 최적화하는 기술을 개발하고 관련 질병의 치료제 개발기반을 구축
• 줄기세포의 연골세포 분화, 탈분화 및 비후화 조절인자구명
• 후생역학적 조절기술을 이용한 효율적 연골 분화 및 비후억제기술 개발
• 생체미세환경을 조절하여 줄기세포의 골 및 연골 분화조건을 최적화하고 줄기세포분화와 효소활성 변화의 관계

( 출처 : 보고서 요약서 2p )

Abstract ▼

Ⅲ. Results
• miR-137, miR-103-3p, miR-200a, miR-210 and miR-4284 control proliferation and differentiation of hADSCs by targeting Cdc42, CDK6/Dicer1, ZEB3, IGFBP3 and HDAC1, respectively.
• MicroRNA-302 induces proliferation and inhibits oxidant-induced cell death in hADSCs through interaction

Ⅲ. Results
• miR-137, miR-103-3p, miR-200a, miR-210 and miR-4284 control proliferation and differentiation of hADSCs by targeting Cdc42, CDK6/Dicer1, ZEB3, IGFBP3 and HDAC1, respectively.
• MicroRNA-302 induces proliferation and inhibits oxidant-induced cell death in hADSCs through interaction with CDKN1A and CCL5.
• BMP2 increases adipogenic differentiation in the presence of dexamethasone, which is inhibited by the treatment of TNF-α in hADSCs
• Transforming growth factor-activated kinase-1 plays an important role on TNF-α (tumor necrosis factor-α) actions in hADSCs, which is mediated by the activation of p38 kinase.
• CTSK, PBX1, DLX1 and FOXC1, of which show differentiatial expression between hBMSCs and hADSCs regulates osteogenic and adipogenic differentiation.
• Characterization Cytl1 and HIF-2α in chondrocyte degenration and stem cell differentiation. Plos Biology (IF 11.771)
• Identification and charcterization of novel regulatory factors (BATF, LOX, ZIP8, MTF1, and NAMPT) involved in the degeneration of chondrcytes and stem cell differentiation. [NAMPT – published in Ann. Rueum. Dis. (IF 9.270); ZIP8 – published in Cell (IF 32.020).
• Characterized that factors involved in cartilage degeneration also regulates stem cell differentiation
• Charcterized regulatory mechanisms of stem cell differentiation by CYTL1 and BATF
• Analyzed the DNA promoter methylation status of TGF-β/BMP(TGF-β1, BMP-2, BMP-7), IGF/FGF(IGF-1, FGF-2), PTHrP/IHH(PTHrP) in chondrogenesis markers between stem cells and chondrocyte.
• Screened the genomewide methylation status of stem cell and chondrocyte using tiling chip promoter array and we set-up database of specific methylation marker with support data for the whole genome analysis.
• We acquired the specific protein binding DNA with histone 3 protein using ChIP-on-ChIP experiments related with chromatin modulation.
• We analyzed the effect of HDAC inhibitor in stem cells and chondrocyte using HDAC inhibitor (SAHA, Trichostatin A), trichostatin A showed significant HDAC inhibition activity.
• Comparison of differentiation between 2D culture system and 3D hydrogel-based system
• Investigation of mechanism of differentiation according to nano/micro substrate
• Investigation of mechanism of differentiation according to ECM component
• Synthesis of fluorescence-inducing biomaterials with general proteolytic activity of DQ-BSA, collagen, and gelatin and the evaluation of the relationship between them

( 출처 : SUMMARY 5p )

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 보고서 요약서 ... 2
• 요 약 문 ... 3
• SUMMARY ... 5
• CONTENTS ... 7
• 목차 ... 8
• 제1장 연구개발과제의 개요 ... 9
• 제2장 국내외 기술개발 현황 ... 24
• 제3장 연구개발수행 내용 및 결과 ... 27
• 제 1 절 1 세부 연구개발 실적 ... 27
• 제 2 절 2 세부 연구개발 실적 ... 67
• 제 3 절 3 세부 연구개발 실적 ... 82
• 제 4 절 4 세부 연구개발 실적 ... 92
• 제4장 목표달성도 및 관련분야에의 기여도 ... 148
• 제5장 연구개발결과의 활용계획 ... 157
• 제6장 연구개발과정에서 수집한 해외과학기술정보 ... 160
• 제7장 연구시설ㆍ장비 현황 ... 163
• 제8장 참고문헌 ... 165
• 끝페이지 ... 168