초록

역유전학 기법을 이용한 고병원성 조류인플루엔자 백신 개발함.

곤충세포 발현을 이용한 고병원성 조류인플루엔자 백신 개발함.

고병원성 조류인플루엔자 백신을 위한 DIVA ELISA 개발함.

오리 불활화 백신 면역원성 향상을 위한 어주번트를 개발함.

오리 불활화 백신 면역원성 향상을 위한 피내접종기를 개발함.

오리분리 살모넬라 티피뮤리움을 백신을 개발함.

(출처 : 요약서 3p)

Abstract ▼

Purpose & Contents
[Goal of the first division : Development of highly pathogenic avian influenza(HPAI) differential(DIVA) vaccine and diagnosis kit for duck use]
- Development of HPAI differential(DIVA) vaccine antigens for duck use
- Development and efficacy evaluation of HPAI differentia

Purpose & Contents
[Goal of the first division : Development of highly pathogenic avian influenza(HPAI) differential(DIVA) vaccine and diagnosis kit for duck use]
- Development of HPAI differential(DIVA) vaccine antigens for duck use
- Development and efficacy evaluation of HPAI differential(DIVA) vaccine for duck
- Prototype production of HPAI differential(DIVA) vaccine for duck use
- Efficacy evaluation and industrialization of prototype HPAI differential(DIVA) vaccine for duck use
- Development of serological HPAI vaccinated/infected objects differentiation (DIVA) kit for duck use
- Vaccine antigen development of food poisoning Salmonella for duck use
- Development and efficacy evaluation of food poisoning Salmonella vaccine
- Prototype production of food poisoning Salmonella vaccine for duck use
- Efficacy evaluation and industrialization of prototype of food poisoning

Salmonella vaccine for duck use
[Goal of the second division : Development of vaccine adjuvant for duck use and improving vaccine delivery methods]
- Development of vaccine adjuvant for ducks that can increase the immunogenicity of inactivated viral vaccine antigen
- Development of vaccine adjuvant for ducks that can increase the immunogenicity of inactivated bacterial vaccine antigen
- Improving vaccine delivery methods that can increase the immunogenicity
- Effectiveness mechanism study of improved vaccine adjuvants and delivery methods for duck

[Goal of the first collaborative : Monitoring and characterization of food poisoning Salmonella derived from domestic duck farms]
- Monitoring of food poisoning Salmonella derived from domestic duck farms
- Characterization of food poisoning Salmonella derived from domestic duck farms
- Efficacy evaluation of Salmonella vaccine using the latest Salmonella isolates

Results
We developed HPAI vaccine using reverse genetics techniques
- We developed HPAI vaccine using insect cell expression
- We developed DIVA ELISA for HPAI vaccine
- We developed vaccine adjuvant for enhancing the immunogenicity of inactivated HPAI vaccine for duck use
- We developed intradermal inoculation device for enhancing the immunogenicity of inactivated HPAI vaccine for duck use
- We developed Salmonella typhimurium vaccine originated from ducks

Expected Contribution
- Securing emergency vaccine for domestic poultry quarantine through the development of HPAI vaccines for duck use

- industrialization of HPAI vaccines for duck use through the collaboration with participating companies and securing the competitiveness of domestic enterprises through the international sale (H5N1 HPAI type epidemic by country: China clade2.3.2, Pakistan clade 2.3.2, Indonesia clade 2.1, 2.3.2, Vietnam clade1, 2.3.2)

- Patent registration of new adjuvants that can increase immunogenicity of inactivated viral and bacterial antigens in ducks and extensive commercial utilization through industrialization in poultry vaccine industry

- Securing academical data through study of vaccine delivery methods that can increase vaccine efficacy in ducks

- Creation of economic value through industrialization and application of food poisoning Salmonella vaccine for duck use

- Contribution to safe food production through Salmonella vaccine application to the duck farms and minimizing risk of food poisoning caused by duck meat

(출처 : SUMMARY 5p)

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 보고서 요약서 ... 3
• 요약문 ... 4
• SUMMARY ... 5
• Contents ... 6
• 목차 ... 7
• 제 1 장. 연구개발과제의 개요 ... 8
• 1절. 연구개발 목표 ... 8
• 2절. 연구개발의 필요성 ... 8
• 3절. 연구개발 범위 ... 13
• 제 2 장. 국내외 기술개발 현황 ... 14
• 1절. 국내외 시장 현황 ... 14
• 2절. 국내외 연구 동향 ... 16
• 3절. 본 연구의 기술 개발 수준 ... 17
• 제 3 장. 연구수행 내용 및 결과 ... 19
• 1절. 1차년도 연구수행 내용 및 결과 ... 19
• 2절. 2차년도 연구내용 및 결과 ... 54
• 3절. 3차년도 연구내용 및 결과 ... 90
• 4절. 연구개발성과 ... 107
• 제 4 장. 목표달성도 및 관련분야 기여도 ... 110
• 1절. 목표달성도 ... 110
• 2절. 관련분야 기여도 ... 113
• 제 5 장. 연구결과의 활용계획 ... 115
• 1절. 산업화 계획 ... 115
• 2절. 특허 확보 계획 ... 115
• 3절. 추가 연구 계획 ... 116
• 제 6 장. 연구과정에서 수집한 해외과학기술정보 ... 117
• 제 7 장. 연구개발결과의 보안등급 ... 124
• 제 8 장. 국가과학기술종합정보시스템에 등록한 연구시설·장비현황 ... 125
• 제 9 장. 연구개발과제 수행에 따른 연구실 등의 안전조치 이행실적 ... 126
• 제 10 장. 연구개발과제의 대표적 연구실적 ... 126
• 제 11 장. 기타사항 ... 128
• 제 12 장. 참고문헌 ... 129
• 별첨 1 연구개발보고서 초록 ... 130
• 끝페이지 ... 131