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Kafe 바로가기주관연구기관 | 경북대학교 KyungPook National University |
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연구책임자 | 이경열 |
참여연구자 | 김지광 , 이관석 , 이석찬 , 이중환 , 최홍수 , 이인용 |
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 | 한국어 |
발행년월 | 2017-02 |
과제시작연도 | 2015 |
주관부처 | 농림축산식품부 Ministry of Agriculture, Food and Rural Affairs(MAFRA) |
과제관리전문기관 | 농림수산식품기술기획평가원 Korea Institute of Planning and Evalution for Technology of Food, Agriculture, Forestry and Fisherie |
등록번호 | TRKO201800009843 |
과제고유번호 | 1545011615 |
사업명 | 농생명산업기술개발 |
DB 구축일자 | 2018-05-26 |
키워드 | 가루이류.식물바이러스.저항성.바코드칩.Whitefly.Plant virus.RNAi.Microsatellite.Barcode chip. |
DOI | https://doi.org/10.23000/TRKO201800009843 |
고위해성 바이러스인 토마토황화잎말림바이러스(TYLCV)의 방제를 위하여 매개충인 가루이류의 바이러스 매개기작, 유전적 변이, 지역별 약제저항성 검정, 중간기주인 잡초의 감염율, 국내 유입 신종 바이러스의 발생, 변이, 확산 등 조사했다. 또한, 정확한 진단을 위하여 LAMP, DNA 칩 등 첨단 진단법을 개발하고 바이러스의 변이 및 복합감염 패턴을 분석했다.. 가루이를 방제하기 위하여 공생균 살충독소 분리, 유인살충식물의 추출물 분리, 녹색 LED 유인등, 님액제의 관주법 등 친환경 방제제을 개발했고 이를 이용하여 가류이류 매개 바
고위해성 바이러스인 토마토황화잎말림바이러스(TYLCV)의 방제를 위하여 매개충인 가루이류의 바이러스 매개기작, 유전적 변이, 지역별 약제저항성 검정, 중간기주인 잡초의 감염율, 국내 유입 신종 바이러스의 발생, 변이, 확산 등 조사했다. 또한, 정확한 진단을 위하여 LAMP, DNA 칩 등 첨단 진단법을 개발하고 바이러스의 변이 및 복합감염 패턴을 분석했다.. 가루이를 방제하기 위하여 공생균 살충독소 분리, 유인살충식물의 추출물 분리, 녹색 LED 유인등, 님액제의 관주법 등 친환경 방제제을 개발했고 이를 이용하여 가류이류 매개 바이러스병의 종합적 방제기술 개발했다.
(출처 : 요약서 3p)
Purpose&Contents
1. Identification of virus-transmission mechanism of whiteflies and development of whitefly control techniques
2. Analysis of genetic diversity and regional pesticide resistance of whiteflies
3. Analysis of genetic diversity of whitefly-transmissible virus and DNA c
Purpose&Contents
1. Identification of virus-transmission mechanism of whiteflies and development of whitefly control techniques
2. Analysis of genetic diversity and regional pesticide resistance of whiteflies
3. Analysis of genetic diversity of whitefly-transmissible virus and DNA chip development
4. Analysis of weed-mediated virus spread mechanism and development of management techniques
5. Development of acute diagnosis techniques of whitefly-transmissible virus and the analysis of multiple virus infection
6. Field monitoring of mutation and multiple infection of whitefly-transmissible virus
7. Development of environment-friendly pesticide for the whitefly control
8. Development of the integrated management system of whitefly-transmissible virus
Results
1. Identification of virus-transmission mechanism of whiteflies and development of whitefly control techniques
○ Physiological and molecular mechanisms of virus-transmission mechanism of whiteflies were identified
- When whiteflies acquisited TYLCV, their development and behavior was increased but the susceptibility against the exposures of high temperature and pesticides was decreased in comparison to non-viruliferous (NV) whiteflies.
Namely, whiteflies enhanced virus transmission rates by active behavior but suffered trade-off expenses at unsuitable environmental conditions.
○ Treatments of RNAi using dsRNA of heat shock protein 70 (hsp70) gene of Bemisia tabaci suppressed its mRNA level and knockdown whiteflies. This technique can be applicable into the whitefly control.
○ Lethal activity of symbiont culture media of entomopathogenic nematodes was determined into the feeding assay of whiteflies using the two-layered parafilm chamber.
- The insecticidal compounds, two anthraquinones, were identified from the culture medium of the symbiont Photorhabdus temperata, and determined its toxicity against the B. tabaci adults.
- Commercial product was developed using the enhancement of these compounds in the symbiont culture media.
○ Whitefly attraction rate was analyzed using various rates of wave length of LED bulbs.
- Green LED light was most attractable for whiteflies and used to develop the LED light trap.
2. Analysis of genetic diversity and regional pesticide resistance of whiteflies
○ Since 2008, the occurrence and genetic diversity of whiteflies has been conducted around the country as well as various countries including southeastern countries, including Bangladesh, Myanmar, Nepal.
- There were three species (B, Q and JpL) in Korea by the analysis of nucleotide sequences of CO, rDNA and endosymbionts
- B-biotype was only present in Goyang, the Q-biotype and JpL were spread around the country, but Q-biotype was habit in various crop plants but the JpL biotype was mostly present in honeysuckle Lonicera japonica.
○ The genetic diversity of the greenhouse whitefly, Trialeurodes vaporariorum, was determined and its species-specific primers were determined for the accurate diagnosis.
○ Pesticide resistance rates were investigated from the collected whiteflies around the country.
- The rates were various and depend on the regions that have different application programs.
3. Analysis of genetic diversity of whitefly-transmissible virus and DNA chip development
○ Analysis of outbreak, symptom and genetic diversity of 7 whiteflytransmissible virus including TYLCV
○ Development of DNA chip for the multiple virus diagnosis
4. Analysis of weed-mediated virus spread mechanism and development of management techniques
○ Due to the wide range of host plants of B. tabaci, we investigated weed species that can be host of TYLCV.
- 17 new host plants were identified including 5 crop species, 12 weed species.
○ The interactive mechanisms of TYLCV-whiteflies–weeds were determined in the field situation and identified that weeds are important as “the green bridge” of the transmission of TYLCV across the corp plants. This suggest the importance of the green bridge control as a part of integral to pest and diseasemanagement.
○ The weed management manual was developed for the control of TYLCV.
5. Development of acute diagnosis techniques of whitefly-transmissible virus and the analysis of multiple virus infection
○ Development of the field-applicable diagnosis of begomovirus.
- LAMP-PCR techniques were developed for the diagnosis of 7 species of begomovirus.
- Multiplex PCR primers were developed for the diagnosis of TYLCV, TbLCV, HYVV, HYVMB.
- TYLCV diagnosis technique was developed from tomato seeds.
○ Geminivirus multi-infection analysis
- Infection clones were developed for the detection of 4 virus that transmitted by whiteflies.
- The range of host plants for TYLCV were determined from the varieties of various plant species.
○ Analysis of the distribution and symptoms of DNA-beta in Korea
- Distribution of geminivirus DNA-beta was determined in Korea and their infection clones were constructed.
- Mutation rate of whitefly-transmitted virus was analysed from the multi-infection of DNA and RNA virus in tomatoes.
6. Field monitoring of mutation and multiple infection of whitefly-transmissible virus
○ The occurrence of TYLCV disease in the tomato fields and also other crop plants in the fields.
○ We firstly identified that TYLCV infects into various tomato seeds available in the public markets of Korea and other countries.
○ various new begomovirus were identified in the fields and those symptomes were described.
7. Development of environment-friendly pesticide for the whitefly control
○ The control efficacy of symbiont culture media of entomopathogenic nematode were determined and commercial products were developed.
○ The leaf extracts of wild tobacco, Nocothiana benthamiana, was used for the production of commercial products of whitefly control.
8. Development of integrated management of whitefly-transmissible virus
○ The integrated management manual of TYLCV and whiteflies was developed for the greenhouse application.
○ The whitefly control efficacy through the irrigation of the neem liquid formula was determined in the tomato greenhouse.
Expected Contribution
1. Contribution
○ Application of the information on the mechanism of vector and virus interaction for the virus and vector management
○ Field application of the data on the occurrence of virus and vector in the country
○ Industrialization and field application of advanced diagnosis techniques for the control of virus and vector in the field
○ Industrialization and field application of environment-friendly pesticides developed in the project
○ Field application of the pest and disease management system in regarding the complex of virus-vector and plant.
2. Expectation
○ The results of this project strengthen international competition in the field of virus-vector diagnosis, interaction and management
○ The results contribute the establishment of the virus-vector management system in the field.
○ The results of this project strengthen international competition in advanced diagnosis techniques of virus and vector.
○ The results can be used for the construction of global virus and vector genetic information system.
○ The results can be used for the improvement of pest and disease management in terms of the virus-vector-plant complex system.
(출처 : SUMMARY 6p)
과제명(ProjectTitle) : | - |
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연구책임자(Manager) : | - |
과제기간(DetailSeriesProject) : | - |
총연구비 (DetailSeriesProject) : | - |
키워드(keyword) : | - |
과제수행기간(LeadAgency) : | - |
연구목표(Goal) : | - |
연구내용(Abstract) : | - |
기대효과(Effect) : | - |
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