[보고서]쌀의 중금속 기준설정 대응 및 흡수이행 저감화 실행규범 연구

박상원

쌀의 중금속 기준설정 대응 및 흡수이행 저감화 실행규범 연구
Research on the countermeasure for establishment of heavy metal criteria in rice and code of practice for the reduction of transition to rice plant 원문보기

보고서 정보
주관연구기관	국립농업과학원 National Institute of Agricultural Sciences
연구책임자	박상원
참여연구자	류지혁 , 오경석 , 김경진 , 이상범 , 최부웅 , 강대원 , 김태중 , 이슬 , 전경미 , 김아름누리 , 백민경 , 박경훈 , 신혜림
보고서유형	최종보고서
발행국가	대한민국
언어	한국어
발행년월	2018-02
과제시작연도	2017
주관부처	농촌진흥청 Rural Development Administration(RDA)
등록번호	TRKO201800043188
과제고유번호	1395049785
사업명	농업기초기반연구
DB 구축일자	2018-12-15
키워드	벼.오염.농경지.비소.저감.rice.contamination.farm land.arsenic.reduction.
DOI	https://doi.org/10.23000/TRKO201800043188

초록 ▼

< 1세부 >
• 쌀의 무기비소 분석법 비교 정립
• 쌀 중 무기비소 추출법
• 수소화물생성법을 이용한 쌀 중 무기비소 분석법
• 벼 품종특성에 따른 비소흡수 요인분석

< 2세부 >
• 유기물 시용과 토양 중 비소 용출 간 상관성 구명
• 유기물 시용이 토양 중 비소 용출과 벼의 비소 흡수에 미치는 영향 구명

< 3세부 >
• 농산물 중 비소 생체이용율 산정방법 정립
• 종자 발아 실험을 통한 중금속(준금속) 독성 평가
• 백미 내 비소의 생체이용율 : 불림/세척 및 조리 효과
• 물벼룩을 이용한 바이오차 및 적색토 존재 여부에 따른 비소,카드뮴 독성 실험
• 지렁이 독성 실험(Earthworm toxicity test)
• 물벼룩을 이용한 카드뮴 독성에 대한 바이오차 영향

< 4세부 >
• 영양소의 비소 물질대사에 미치는 영향평가
• 곡류 2종(쌀,현미)의 α-amylase를 이용한 무기비소 추출
• 곡류가 무기비소의 발암성 저감에 미치는 영향 연구

( 출처: 보고서 요약서 3p )

Abstract ▼

□ Purpose&Contents
○ Low absorption type and high absorption type are selected for the low absorption type and high absorption type. The low absorption type is used for enhancing the safety of agricultural products in the soil contaminated with heavy metals. The high absorption ability should be utilized in the botanical restoration method of contaminated soil
○ Investigation of heavy metals in cultivation environment and agricultural products
○ To select heavy metals and low water absorption capacity and absorption capacity of rice varieties

□ Results
○ Development of analytical method using HG-ICP / MS for inorganic arsenic analysis of rice (Policy proposal)
○ Obtain safety management data on heavy metals in export rice production complex to China (Farming information)

□ Expected Contribution
○ To obtain data for domestic research to reduce the absorption of heavy metals (arsenic) in rice and obtaining data for countermeasures to prevent and reduce arsenic contamination of rice by the Codex Committee on Food Pollutants

( 출처: SUMMARY 4p )

목차 Contents

표지 ... 1
제 출 문 ... 2
보고서 요약서 ... 3
국 문 요 약 문 ... 4
Summary ... 4
목차 ... 5
제 1 장 연구 개발 과제의 개요 ... 6
제1절 연구 개발 목적 ... 6
제2절 연구 개발의 필요성 ... 7
제3절 연구 개발 범위 ... 7
제 2 장 국내외 기술개발 현황 ... 8
제 3 장 연구 수행 내용 및 결과 ... 10
제 4 장 목표달성도 및 관련분야 기여도 ... 92
제1절 : 목표대비 달성도 ... 92
제2절 : 정량적 성과 ... 92
제 5 장 연구 결과의 활용 계획 ... 93
제 6 장 연구 과정에서 수집한 해외 과학 기술 정보 ... 93
제 7 장 연구 개발 결과의 보안 등급 ... 94
제 8 장 국가과학기술지식정보서비스에 등록한 연구시설·장비 현황 ... 94
제 9 장 연구개발과제 수행에 따른 연구실 등의 안전조치 이행실적 ... 95
제 10장 연구개발과제의 대표적 연구실적 ... 95
제 11 장 기타사항 ... 97
제 12 장 참고문헌 ... 99
끝페이지 ... 101

표/그림 (115)

표 Manhattan plot of genomewide association scan Cd content
표 Some genes Positon associated with the phenotypes
표 Number of haplotype variation in genes related Cadmium among the 137 accessions of rice core set. (A) Nucleotide variation in the coding region, (B) Phylogenetic tree of the Cadmium, (C) Cadmium content group
표 Manhattan plot of genome wide association scan As content
표 Organic matter treatment to each pot
표 Organic matter treatment to each experimental plot
표 Metal(loid) content of soils used in this study (mg kg-1)
표 Organic matter treatment and construction of field scale experimental plots
표 Status of field experiment conducted in 2016 including soil solution sampling
표 Status of field experiment conducted in 2017 including soil solution sampling
표 Organic matter treatment to each experimental plot
표 Selected Chemical properties of study soils
표 Heavy metal(loid) content of study soils
표 Heavy metal(loid) content of organic matters used in this study (mg/kg DW)
표 C/N ratio of organic matters used in this study
표 Changes in arsenic content in soil solutions for each treatment after rice transplanting
표 Changes in Fe content in soil solutions for each treatment after rice transplanting
표 Changes in Mn content in soil solutions for each treatment after rice transplanting
표 Changes in As content in soil solutions between 1X and 3X application rate for each treatment after rice transplanting
표 Changes in As content in soil solutions between 1X and 3X application rate for swine liquid manure treatment after rice transplanting
표 Changes in As content in soil solutions for each treatment 67 days after rice transplanting. Values are presented as the mean ± SD (n=3). Single asterisks indicate significant differences (p < 0.05) compared to the control value; double asterisks indicate significant differences (p < 0.05) upon comparison between 1x and 3x treatment for a given group
표 Changes in As content in soil solutions for each treatment 91 days after rice transplanting. Values are presented as the mean ± SD (n=3). Single asterisks indicate significant differences (p < 0.05) compared to the control value; double asterisks indicate significant differences (p < 0.05) upon comparison between 1x and 3x treatment for a given group
표 Changes in As content in soil solutions for each treatment 112 days after rice transplanting. Values are presented as the mean ± SD (n=3). Single asterisks indicate significant differences (p < 0.05) compared to the control value; double asterisks indicate significant differences (p < 0.05) upon comparison between 1x and 3x treatment for a given group
표 Effects of organic matter on arsenic content in soil solutions at 67, 91, 112 days after rice transplanting. Different letter within a column indicates significant differences at p < 0.05 level (Duncan’s multiple range test)
표 Changes in pH of soil solutions for each treatment
표 Changes in dissolved organic carbon content of soil solutions for each treatment
표 Changes in dissolved organic carbon content of soil solutions between 1x and 3x application rate for each treatment
표 Changes in dissolved organic carbon content of soil solutions between 1x and 3x application rate for each treatment
표 Changes in dissolved organic carbon content of soil solutions between 1x and 3x application rate for each treatment
표 Mean tAs content in brown rice from each treatment of organic matter (Asterisks indicate significant differences (p < 0.01) compared to the control value)
표 Distribution of arsenic species in soil solutions after rice planting
표 Changes in tAs in soil solutions from highly polluted soils
표 Changes in tAs in soil solutions from moderately polluted soils
표 Changes in DOC in soil solutions from highly polluted soils
표 Changes in DOC in soil solutions from moderately polluted soils
표 Total As content in brown rice form each treatment. Asterisks indicate significant differences (p < 0.05) compared to the control value
표 Inorganic As content in brown rice form each treatment (mg/kg)
표 C/N ratio of organic matters used in this field study
표 Changes in inorganic As content in soil solutions from each treatment at 78days after rice planting
표 Total As content in brown rice form each treatment
표 Total As content in white rice form each treatment. Asterisks indicate significant differences (p < 0.05) compared to the control value
표 Inorganic As content in white rice form each treatment (mg/kg)
표 Rice before cooking
표 Seed germination test
표 Germination % of lettuce seedlings in the presence of treated soils
표 Root length of lettuce seedlings in the presence of treated soils and arsenite
표 Bioavailable and bioaccessible arsenic species in YS1 raw and cooked rice
표 Bioavailable and bioaccessible arsenic species in YS2 raw and cooked rice
표 Toxicity experiment using Daphnia magna
표 Mortality percentage of daphnias in the presence of biochars and red soil
표 Mortality percentage of daphnias in the presence of arsenite (5 mg/L)
표 Survival rate of daphnias against cadmium toxicity in the presence of 1% biochar at various temperatures after 24 hours
표 Survival rate of daphnias against cadmium toxicity in the presence of 0.1% biochar at 20℃ and 25℃ after 24 and 48 hours
표 Set up of avoidance experiment
표 Number of worms remaining after the completion of avoidance test in iron-rich soils (FeO: Iron oxide; FeZr: Iron-zirconium oxide; RS: Red soil; NRE: Natural red earth)
표 Number of worms remaining after the completion of avoidance test in iron-rich soils (FeO: Iron oxide; RS: Red soil; PMBC 450: Poultry manure biochar at 450℃ ; HVBC 450: Hairy vetch biochar at 450℃)
표 Number of worms remaining after the completion of avoidance test in iron-rich soils (HVBC 450: Hairy vetch biochar at 450℃; HVBC 700: Hairy vetch biochar at 700℃; PMBC 450: Poultry manure biochar at 450℃; PMBC 700: Poultry manure biochar at 700℃)
표 Survival rate of daphnias against cadmium toxicity in the presence of 1% biochar at various temperatures after 24 hours
표 Survival rate of daphnias against cadmium toxicity in the presence of 0.1% biochar at various temperatures after 48 hours
표 Effect of cadmium (1 mg/L) on the mortality rate (%) of daphnias in the presence of biochar
표 Effect of cadmium (1 mg/L) on dissolved oxygen levels in the presence of biochar
표 Effect of cadmium (1 mg/L) on the number of offspring in the presence of biochar
표 Size differences between the control daphnia and the treatments
표 Effect of cadmium (0.1 mg/L) on the mortality rate (%) of daphnias in the presence of biochar
표 Effect of cadmium (0.1 mg/L) on the number of offspring in the presence of biochar
표 Treatment groups
표 Oral dose of the test substance
표 Drinking water exposure concentration
표 Test schedule for drinking water arsenite exposure
표 Cages for drinking water exposure treatments
표 Average body weight during oral administration treatment
표 Average water consumption during oral administration treatment
표 Average food consumption during oral administration treatment
표 Urinary excretion of inorganic arsenic and its metabolites in rats following oral administration of AsIII
표 Urinary excretion of inorganic arsenic and its metabolites in rats following oral administration of AsIII
표 Urinary excretion of inorganic arsenic and its metabolites in rats following oral administration of AsIII
표 Urinary excretion of inorganic arsenic and its metabolites in rats following oral administration of AsIII
표 Average body weight during 4 weeks of exposure to AsIII in drinking water
표 Average water consumption during 4 weeks of exposure to AsIII in drinking water
표 Average food consumption during 4 weeks of exposure to AsIII in drinking water
표 Urinary excretion of inorganic arsenic and its metabolites following a 24 hr cumulative urine collection after 2 week of exposure to AsIII in drinking water
표 Urinary excretion of inorganic arsenic and its metabolites following a 24 hr cumulative urine collection after 2 week of exposure to AsIII in drinking water
표 Urinary excretion of inorganic arsenic and its metabolites after 4 week of exposure to AsIII in drinking water
표 Urinary excretion of inorganic arsenic and its metabolites after 4 week of exposure to AsIII in drinking water
표 Urinary excretion of inorganic arsenic and its metabolites following a 0∼24 hr cumulative urine collection after 4 week of exposure to AsIII in drinking water
표 Urinary excretion of inorganic arsenic and its metabolites following a 0∼24 hr cumulative urine collection after 4 week of exposure to AsIII in drinking water
표 Urinary excretion of inorganic arsenic and its metabolites following a 24∼48 hr cumulative urine collection after 4 week of exposure to AsIII in drinking water
표 Urinary excretion of inorganic arsenic and its metabolites following a 24∼48 hr cumulative urine collection after 4 week of exposure to AsIII in drinking water
표 Creatinine concentration in urine sample collected from oral administration
표 Creatinine concentration in urine samples collected from drinking water AsIII treatment
표 Extraction of crop
표 Operating condition for the HPLC-ICP-MS
표 Construction and SOP of micronucleus test (in vitro)
표 Analysis method and flow cytometer
표 Construction and SOP of comet assay(in vitro) method
표 Group
표 Group
표 Recovery of crop extraction method using CRM
표 Inorganic arsenic content and yield of extraction using α-amylase
표 Flow cytometry analysis of MMC treated CHL cell
표 Micronucleus test
표 Micronucleus generation
표 Inorganic arsenic content of two crops(white rice, brown rice)
표 Morphology of HaCaT cells (human keratinocytes)
표 DNA damage induced by MMS with different concentration in HaCaT cells (1 × 105/well) and measured by alkaline comet assay. (a)DMSO, (b)50, (c)100, (d)200, (e)500 and (f)1000 μM of MMS-treated cells
표 DNA damage by MMS
표 DNA damage by inorganic arsenic
표 DNA damage by crops
표 Down regulation of crops(white rice, brown rice) of in HaCaT cells
표 Micronucleus
표 Micronucleus generation
표 Micronucleated immature red blood cells
표 Micronucleus generation by AsⅢ and crops(white rice, brown rice)
표 Micronucleus generation
표 China’s Maximum Levels for Contaminants in Foods

과제명(ProjectTitle) :	-
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연구내용(Abstract) :	-
기대효과(Effect) :	-

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연합인증

쌀의 중금속 기준설정 대응 및 흡수이행 저감화 실행규범 연구
Research on the countermeasure for establishment of heavy metal criteria in rice and code of practice for the reduction of transition to rice plant 원문보기