[보고서]식용개구리 대량사육 및 가공ㆍ이용기술 체계 확립 연구

이진구

보고서 정보

주관연구기관

경기도농업기술원

연구책임자

이진구

참여연구자

강창성 , 임갑준 , 장재은 , 윤승환 , 황지은 , 박대식 , 최우진 , 박일국 , 김영성 , 김경식 , 권태은

보고서유형

최종보고서

발행국가

대한민국

언어

한국어

발행년월

2018-02

과제시작연도

2017

주관부처

농촌진흥청
Rural Development Administration(RDA)

연구관리전문기관

농촌진흥청
Rural Development Administration

등록번호

TRKO201800043492

과제고유번호

1395050169

사업명

지역농업연구기반및전략작목육성(보조/경제)

DB 구축일자

2019-05-18

키워드

개구리.사육.질병.먹이장치.가공.frog.rearing.disease.feeding device.processing .

DOI

https://doi.org/10.23000/TRKO201800043492

표 Characteristics of frog farms in Korea
표 Improving of 「Guideline for the Management of Artificial Propagation of Wild Animals」
표 Developmental characteristics of tadpole of R.dybowskii according to temperature
표 Developmental characteristics of R.dybowskii according to temperature and density
표 Induction of egg-laying of R.dybowskii by hormone treatment
표 Degree of eating of R.dybowskii by color and physical properties of feed
표 Eating rate of R.dybowskii by type and color of artificial food additive
표 Composition ratio of artificial diet for R.dybowskii
표 Ingredient ratio of artificial diet for R.dybowskii
표 Number of the Rana dybowskii frogs and tadpoles which were collected from inside and outside farms at Inje, Goesan, and Gongju for the study and their physical parameters
표 Relationships between the number of bacterial and fungal colonies among the different body parts of Rana dybowskii frogs collected from inside and outside frog farms at Inje and Geosan and between the numbers and their condition factor
표 Relationships between the number of fungal or bacterial colony on the skin (A) and in the heart (B) and the condition factor of the Rana dybowskii frogs that were collected from inside and outside frog farms at Inje and Goesan
표 Number of bacterial colony (A) on the skin and in the gut and fungal colony (B) on the skin and in the liver of the Rana dybowskii that were collected from inside and outside frog farms in Inje and Goesan. Number of bacterial and fungal colony in Goesan was greater than that in Inje, but there was no difference between inside and outside frog farms at both Inje and Goesan
표 Number of bacterial (A) and fungal (B) colony on the skin and inside the body of the Rana dybowskii tadpoles that were collected from inside and outside frog farms at Inje and Gongju. Number of bacterial colony was different between inside and outside frog farms at both Inje and Gongju and the number of fungal colony was tended to be different between Inje and Gongju
표 List of the bacteria identified from the samples of water and the skin and internal organs of the Rana dybowskii tadpoles which were collected from outside and inside frog farms at Inje and Gongju
표 An acanthocephalan parasite in the stomach of the adult Dybowskii’s brown frog, collected inside of commercial frog farms at Goesan, indicated by an arrow (A) and the collected acanthocephalan parasites shown under the dissecting microscope(40X, B). In B, the proboscis of an acanthocephala is indicated by an arrow
표 Two types (A, B) of acanthocephalan parasites collected from the Dybowskii’s brown frogs and the morphological characteristics of an Centrorhynchus acanthocephala (C, D). Bar=400 μm. Only two larvae in B plate type were found and excluded in further analysis
표 A maximum likelihood (ML) tree based on the 18S ribosomal RNA (18S rRNA) genomes of the collected acanthocephala(G525CS) in addition to 15 other acanthocephalan species. 18S rRNA sequences of acanthocephalalan species used in this phylogenetic analysis were downloaded from GeneBank the accession number indicated after the scientific name of each species. The Bayesian posterior probabilities (above) and bootstrap value (below) are denoted on each branch
표 Relationships among the number of acanthocephalans found in each body cavity, stomach, small and large intestine, and combined total number and the condition factor of the Dybowskii’s brown frogs examined (n=60)
표 Comparison of the number of acanthocephalans found in body cavity, stomach, small and large intestine of the Dybowskii’s brown frogs between inside and outside of the frog farms (A) and between males and females (B)
표 Comparison of acanthocephalan infection rates (A) and the number of acanthocephalans found (B) in body cavity, stomach, small and large intestine of the Dybowskii’s brown frogs among Inje, Goesan, Gongju, and Boryeong frog farms. *, P <0.05 in post-hoc test
표 Sites where narrow-mouth frog (Daejeon) and tree frogs (Hapcheon-gun) are captured
표 Tadpoles collected in Woogok-ri, Yongju-myeon, Hapcheon-gun, Gyeongsangnam-do. (A) Tree frog tadpole with ecchymosis (arrows), one of representative ranavirus infection symptoms. (B) Tree frog tadpole with exophthalmos symptom (yellow arrow) and swelling body (red arrow)
표 Result of chytrid fungus detection using PCR from the eleven tree frog larvae collected in Yongju-myeon, Hapcheon-gun. M: Marker (100 bp), 1-11: tadpole samples, N: Negative control. Batrachochytrium dendrobatidis was not detected from all samples
표 Result of ranavirus detection using PCR from the six Narrow-mouth frogs collected in Eoeun-dong, Daejeon. M: Marker (100 bp), 1-6: Samples, P: Positive control (red arrow), N: Negative control. Three frogs, indicated by yellow arrows showed ranavirus positive responses in PCR
표 Result of ranavirus detection using PCR from three narrow-mouth frog larvae collected in Eoeun-dong, Daejeon. M: Marker (100 bp), 1-3: Samples, P: Positive control (red arrow), N: Negative control. Ranavirus was not detected at all
표 Result of ranavirus detection using PCR from eleven tree frog larvae collected in Yongju-myeon, Hapcheon-gun. M: Marker (100 bp), 1-11: Samples, P: Positive control (red arrow), N: Negative control. One frog, indicated by yellow arrow showed ranavirus positive responses in PCR
표 Location where the mass-mortality of Huanren frog (Rana huanrenensis) tadpoles occurred at Inje, Kangwon, South Korea in June 2015. Yellow arrows in A, B, C indicate the areas where dead tadpoles were mainly found (A, B, C) and those in D indicate dead tadpoles among the pebbles. Insertion in D shows a dead tadpole in large
표 List of bacterial species identified from the skin, internal tissue, and digestive canal samples of two live and four dead Huanren frog (Rana huanrenensis) tadpoles collected from the mass-mortality site on June 18, 2015. We successfully analyzed 49 bacterial sequences and determined species (mean bp: 1193 bp, range: 768-1389 bp) out of total 54 colonies (each three colonies × three different organs × six tadpoles)
표 Results of custom BLAST using concatenated two ranavrius sequences (Accession Number: KY264305 + KY496311, KY264-24 + KY496311) obtained from Huanren frog (Rana huanrenensis) tadpolesin this study and 16 sequences from GenBank. The sequence lengths compared were identical as 1099 bp. For the accession number of each gene from GenBank, see Figure 2
표 Phylogenetic tree based on partial MCP DNA sequences of ranavirus. The sequences (1099 bp) obtained from Huanren frog (Rana huanrenensis) tadpolesin this study are in larger, bold font and showed over 99% identity with the MCP gene sequence of Rana catesbeianavirus JP, Frog virus 3, Rana grylioiridovirus, Ranavirus KRV-1, Soft-shelled turtle iridovirus in a descendant identity order. Numbers at the end of the taxon name refer to GenBank accession numbers. Numbers on the branches represent support values for the major groups—ML bootstrap support, followed by Bayesian posterior probabilities
표 Comparison of the growth rate of R. dybowskii ’s tadpoles based on different treatments of control, OCT, propolis I and II exposure
표 Comparison of the metamorphosing rate of R. dybowskii’s tadpoles based on different treatments of control, OCT, propolis I and II exposure
표 Comparison of the snout-vent length and body weight of metamorphosed R. dybowskii’s juveniles which reared at different treatments of control, OCT, propolis I and II exposure
표 Comparison of the number of bacterial (left) and fungal (right) colony on the skin, in the heart and liver of metamorphosed R. dybowskii ’s juveniles which reared at different treatments of control, OCT, propolis I and II exposure
표 Comparison of the degree of hernia after control, UV, and UV+propolis treatments in R. dybowskii’ frogs. The condition was only improved in the group of UV+propolis treatments
표 Improved hernia in R. dybowskii frog after (B) UV+propolis treatments
표 Improved edema in R. japonica frog after (B) propolis treatments
표 Growth rate (SVL, A) and time to metamorphosis (B) based on different temperature treatments
표 Snout-vent length and body weight of metamorphosed juveniles based on different temperature treatments
표 Number of bacterial (A) and fungal (B) colonies on the skin, in the liver and heart of metamorphosed juveniles, reared at different temperature treatments
표 Growth rate (SVL, A) and time to metamorphosis (B) based on different tadpole density treatments
표 Number of bacterial (A) and fungal (B) colonies on the skin, in the liver and heart of metamorphosed juveniles, reared at different tadpole density treatments
표 Characteristics of feeding methods for frogs
표 Characteristics of feeding device types for frogs
표 Schematic of artificial diet feeding device using the vacuum suction pipe for frog
표 Feeding effects of artificial diet with feeding device and natural food of R.dybowskii
표 Proportion of frogs eating the artificial diets with feeding device(%)
표 Economic analysis according to feeding method of frog
표 Freeze-dried frogs and frog powder
표 proximate composition of edible frogs
표 Minerals of frogs and major protein sources (mg%)
표 Amino acid profile of edible frogs
표 Frog cuisine in Vietnam and China
표 Health functional food utilizing local produce
표 Various types of small packaged HMR
표 HMR　Market in Major Country (Unit: $ Million)
표 HMR　Market in the world (Unit: $ Million)
표 HMR　Market in Korea (Unit: KRW million)
표 RTC　Market in Korea (Unit: KRW million)
표 Frequency of Retort Food Purchase by Consumers in Korea (Unit: %)
표 Consumer Retort Food Consumption in Korea (Unit:%)
표 Processed Food Consumption Trend
표 Online consumer awareness survey on frog processed food development
표 Analysis of social big data related to breakfast
표 Cooked frogs cooked in a variety of cooking methods
표 Texture properties of frog dish according to cooking method
표 Freeze dried edible frog powder
표 Various menus using edible frogs
표 Development of recipe using edible frog powder
표 demonstration of edible frog cushion
표 Herbal medicine ingredients added to improve the taste
표 Taste Evaluation of Liquid Retort Products and Improved Products
표 Comparison of improved products with liquid retort products
표 Appearance and flavor Evaluation of Liquid Retort Products and Improved Products
표 Consumer preference of edible frog liquid extract and powder
표 Formula for Jook with edible frog (Rana dybowskii)
표 Procedure of making J ook added with edible frog (Rana dybowskii)
표 Appearance of J ook added with edible frog (Rana dybowskii) flour
표 pH of J ook added with edible frog (Rana dybowskii) flour
표 Hunter’s color value of Jook added withf edible frog (Rana dybowskii) flour
표 Spreadability of J ook added with edible frog (Rana dybowskii) flour
표 Consumer acceptablitity scores for edible frog (Rana dybowskii) Jook
표 Instant Jook market in Korea
표 Characteristics of commercial protease
표 SDS-PAGE of frog proteins
표 Characteristics of frog protein hydrolysates by commercial enzyme combinations
표 Process for the production of frog protein hydrolyzate without off-flavor

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[국가R&D연구보고서] 식용개구리 대량사육 및 가공ㆍ이용기술 체계 확립 연구
Study of mass rearing, disease management and processing system for industrialization of Amphibian 원문보기

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[국가R&D연구보고서] 식용개구리 대량사육 및 가공ㆍ이용기술 체계 확립 연구 Study of mass rearing, disease management and processing system for industrialization of Amphibian 원문보기

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Study of mass rearing, disease management and processing system for industrialization of Amphibian 원문보기

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