초록 ▼

1. 암세포의 대사조절 신규 단백질(PIN1, DPP4, LPIN1 등) 3종 이상 발굴 및 관련 신호전달 규명
2. 대사조절 단백질 DPP4의 억제제이자 당뇨병 치료제인 Sitagliptin의 암세포의 대사조절을 통한 항암효과 규명
3. 지질대사조절 단백질 LPIN1의 억제제인 propranolol(고혈압 치료제) 의 지질대사 억제기전을 통한 항암효과 규명
4. Tamoxifen, Vemurafenib, Cisplatin, Doxorubicin등 4종 이상의 항암제 내성 세포주 구축
5. 내성암세포에서 Pin

1. 암세포의 대사조절 신규 단백질(PIN1, DPP4, LPIN1 등) 3종 이상 발굴 및 관련 신호전달 규명
2. 대사조절 단백질 DPP4의 억제제이자 당뇨병 치료제인 Sitagliptin의 암세포의 대사조절을 통한 항암효과 규명
3. 지질대사조절 단백질 LPIN1의 억제제인 propranolol(고혈압 치료제) 의 지질대사 억제기전을 통한 항암효과 규명
4. Tamoxifen, Vemurafenib, Cisplatin, Doxorubicin등 4종 이상의 항암제 내성 세포주 구축
5. 내성암세포에서 Pin1 단백질억제제를 포함한 3종 이상의 생리활성 억제제의 항암효과 규명
6. British Journal Pharmacology (JCR10%), Carcinogenesis(JCR20%)등 관련 분야 우수 논문 발표
7. 석사학위 4명, 박사학위 2명의 우수 연구인력 양성
8. 내성 연구 분야에서 항암제 개발연구에 새로운 지표 제시

(출처 : 요약서 3p)

Abstract ▼

□ Purpose&Contents
The final goal of this study is to determine the molecular mechanism of cancer cell metabolic reprogramming of cancer cell by tumor microenvironment, and to provide fundamentals in developing advanced therapeutics against resistance of anti-cancer drug. The goal will be approac

□ Purpose&Contents
The final goal of this study is to determine the molecular mechanism of cancer cell metabolic reprogramming of cancer cell by tumor microenvironment, and to provide fundamentals in developing advanced therapeutics against resistance of anti-cancer drug. The goal will be approached by investigating the signaling pathway network of Pin1 and transcriptional regulation by metabolic reprogramming of cancer cells in relation to their noble roles in cancer pathogenesis using cellular and animal cancer models.

□ Results
❍ The aim of this study is to contribute to the development of inhibitors of cancer metabolic regulation and increase the understanding of anti-carcinogenesis through the inhibition of cancer metabolic signaling by revealing the regulation mechanism of cancer cell metabolism at the molecular level.
❍ In the first year of the study, the molecular mechanism of breast cancer proliferation was identified by illustrating the role of DPP4 as a cancer metabolic target protein. In this process, the relationship between DPP4 and Pin1 is clarified and the inhibitory effect of sitagliptin, an inhibitor of DPP4, on the cancer cells is investigated.
❍ In addition, in the second year of the study, we identified novel metabolic control protein, LPIN1, and identified the pathogenesis of cancer proliferation and molecular signaling regulated by LPIN1. These results have established a network of novel metabolic regulatory proteins for cancer cells and further investigated the anticancer effects of propranolol, a LPIN1 inhibitor, which is used for hypertension treatment.
❍ In the third year of the study, we investigated the mechanism of Pin1-induced resistance against vemurafenib and identified a novel anti-cancer strategy by PIN1 inhibitor, ATRA in melanoma.
❍ These results were published in the fields of pharmacology (JCR 10%) and oncology (Carcinogenesis, JCR 20%) during the period of the study, and researchers for four master degrees and two Ph. D. researchers were trained.
❍ Furthermore, based on the results of the present study, which identifies the target protein that induces the reorganization of the metabolic program ofcancer cells, it has established a foothold for collaboration with a famous domestic pharmaceutical company to develop the novel anti-cancer drug targeting various anti-cancer drug resistance.

□ Expected Contribution
❍ Leading the field of anti-cancer drugs for anti-cancer resistance by 1) finding new pharmacological target, 2) diagnostic tools, 3) lead compounds applying advanced research techniques in a collaborative manner
❍ Since this research will identify the novel therapeutic targets for the new drug development on the cancer development, metastasis, and anticancer resistance, it will develop the phytochemicals targeting cancer

(출처 : SUMMARY 5p)

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 보고서 요약서 ... 3
• 국문 요약문 ... 4
• SUMMARY ... 5
• CONTENTS ... 6
• 목차 ... 7
• 1. 연구개발과제의 개요 ... 8
• 1-1 연구개발 목적 ... 8
• 1-2 연구개발의 필요성 ... 10
• 1-3 연구개발 범위 ... 13
• 2. 국내외 기술개발 현황 ... 15
• 3. 연구수행 내용 및 결과 ... 16
• 3-1. 연구개발 추진전략 및 방법 ... 16
• 3-2. 연구개발 추진체계 ... 18
• 3-3. 연구개발 추진일정 ... 19
• 3-4 연구개발내용 ... 20
• 3-5 연구개발성과 ... 37
• 4. 목표달성도 및 관련분야 기여도 ... 39
• 4-1 목표 달성도 ... 39
• 4-2 관련분야 기여도 ... 40
• 5. 연구결과의 활용계획 ... 41
• 6. 연구과정에서 수집한 해외과학기술정보 ... 42
• 7. 연구개발성과의 보안등급 ... 43
• 8. 국가과학기술종합정보시스템에 등록한 연구시설·장비 현황 ... 43
• 9. 연구개발과제 수행에 따른 연구실 등의 안전조치 이행실적 ... 44
• 10. 연구개발과제의 대표적 연구실적 ... 45
• 11. 기타사항 ... 45
• 12. 참고문헌 ... 45
• 별첨: 실적 증빙자료 ... 46
• 별첨: 기타 첨부서류 ... 52
• 끝페이지 ... 61