초록 ▼

본 연구에서는 글로벌 기후변화에 대응하면서 식량문제 등을 해결하기 위하여 국내외 조건 불리지역 (건조지역, 고 염분지역, 오염지역 등)에 잘 자라면서 고부가가치를 창출하는 산업용 식물 (고구마, 알팔파, 포플러 등)을 개발하기 위한 환경재해내성 유전자 확보와 기능분석, 환경재해 내성 산업용 식물 개발 등 식물생명공학 원천기술 개발과 국제협력기반을 구축하여 다음과 같은 중요한 결과를 얻었다.

1. 환경스트레스 반응 유전자 네트워크 분석 및 유용유전자 기능분석:
(1) 복합재해내성 IbOr 유전자의 기능분석: 해당분야

본 연구에서는 글로벌 기후변화에 대응하면서 식량문제 등을 해결하기 위하여 국내외 조건 불리지역 (건조지역, 고 염분지역, 오염지역 등)에 잘 자라면서 고부가가치를 창출하는 산업용 식물 (고구마, 알팔파, 포플러 등)을 개발하기 위한 환경재해내성 유전자 확보와 기능분석, 환경재해 내성 산업용 식물 개발 등 식물생명공학 원천기술 개발과 국제협력기반을 구축하여 다음과 같은 중요한 결과를 얻었다.

1. 환경스트레스 반응 유전자 네트워크 분석 및 유용유전자 기능분석:
(1) 복합재해내성 IbOr 유전자의 기능분석: 해당분야 상위 10%이내 SCI논문 3편, 특허 2건
- IbOr 유전자/단백질이 높은 chaperone 활성이 있음을 최초로 규명
- IbOr 단백질이 고온 등 스트레스 조건에서 카로티노이드 생합성에 중요한 IbPSY와 결합을 규명
- IbOr 단백질이 고온 등 스트레스 조건에서 광합성 광계II 구성단백질인 IbPsbP와 결합을 규명
- IbOr R96H 변이체는 IbOr보다 카로티노이드 생산에 월등히 높은 것임을 확인 (PCT출원 준비).
* IbOr유전자는 47℃ 고온, 건조, 고 염분, 산화스트레스에 강한 내성을 가지며, 모든 식물에 적용할수 있어, 기후변화 대응 산업용 식물개발에 중요한 플렛트폼(Industrial platform) 기술로 평가됨.
* KBS뉴스광장 등 주요언론에 보도 (KRIBB 보도자료 배포: 2017년 6월 22일)
(2) 환경스트레스 관련 MAPK스트레스 신호전달네트워크 분석: SCI 논문 1편 (1편 준비)
(3) 저온스트레스 내성 유전자 네트워크 분석: SCI 논문 1편 (1편 투고, 2편 준비)
(4) 고구마 토코페롤 합성 전체유전자 분리 및 기능분석: SCI 논문 1편

2. 환경스트레스 내성 형질전환식물 개발 및 환경재배내성 특성분석:
(1) 형질전환(IbOr, AtP3B etc.) 고구마(5종) 개발, 환경재해내성 특성 분석: SCI 논문 5편
(2) 형질전환(IbOr, AtABF3 etc.) 알팔파(2종) 개발, 환경재해내성 특성분석: SCI 논문 2편
(3) 형질전환(PagGI, IbLEA14 etc.) 포플러(3종) 개발, 환경재해내성 특성분석: SCI 논문 3편
* GI포플러는 Plant Biotechnology Journal (IF=7.44, 상위 5.5% 논문) 게재
* YTN 등 주요 언론보도 (KRIBB 보도자료 배포: 2016년 11월 22일)

3. 글로벌 협력네트워크 구축:
(1) 중국과 협력연구 구축: 중국과학원 물토양보존연구소, 중국농업과학원 고구마연구소 등
(2) 카자흐스탄과 협력연구 구축: 국가 식물생명공학연구소, 국가 생명공학연구소 등
(3) 터키 Yeditepe 대학과 고구마 생명공학 기반구축
(4) AASSA(아시아한림원연합회), TRAS(한중일고구마연구협의회) 협력구축 등

(출처 : 요약서 3p)

Abstract ▼

4. Results
1. Analysis of gene networks in response to environmental stresses and functional study of useful genes
1) Functional study of IbOr gene involved in multiple abiotic stresses:
(1) The functions of IbOr gene involved in accumulation of carotenoids were firstly chracterized in term

4. Results
1. Analysis of gene networks in response to environmental stresses and functional study of useful genes
1) Functional study of IbOr gene involved in multiple abiotic stresses:
(1) The functions of IbOr gene involved in accumulation of carotenoids were firstly chracterized in terms of molecular physiology and biochemistry. In particular, IbOr protein have a strong molecular chaperone activity.
(2) IbOr protein interacts with phytoene synthase (PSY), which is most important enzyme in the early steps of carotenoid biosynthetic pathway under stress conditions such as high temperature and oxidative stress. Thus the biosynthesis of carotenoids is normally occurred under stress conditions owing to IbOr protein. The results was published in Scientific Reports (2016).
(3) IbOr protein also interacts with IbPsbP protein, which is one of important protein complex in PSII system under stress conditions such as high temperature and oxidative stress. Thus the photosynthesis is normally occurred under stress conditions owing to IbOr protein. The results was published in Frontiers in Plant Science (2017).
(4) “The functional study of IbOr and its biotechnological application”by PI as an invited speaker was highly evaluated at 2017 International Plant ROS/NO Conference. The review paper on plant Or genes was invited and published in Journal of Experimental Botany (2018).
(5) In conclusion, IbOr gene with multiple tolerance to various abiotic stress such as high temperature, drought stress, high salt stress and oxidative stress can be applicable to all kinds of plant species to develop industrial transgenic plants to cope with climate change as an industrial platform technology.

2) MAP kinase signaling networks in response to environmental stresses in sweetpotato:
(1) The mitogen-activated protein (MAP) kinase module plays a critical role in diverse plant stress responses. We functionally characterized biotic and abiotic stress-responsive MAP kinase genes, IbMPK3 and IbMPK6 from sweetpotato. The results was published in Plant Physiology and Biochemistry (2016).
(2) In addition, IbWRKY proteins phosphorylated by IbMPK3/IbMPK6 were identified and are under characterization. The results is preparation for submission to SCI Journal.

3) Gene networks analysis in response to low temperature stress in sweetpotato:
(1) Tropical origin “sweetpotato” is sensitive to low temperature. The storage of tuberous roots during low temperature is a problem for industrialization of sweetpotato. In this study, transcriptome profiling was carried out using storage roots and leaf tissues treated with low temperature.
(2) By transcriptome of tuberous roots stored at optimal (13℃) or low temperature (4℃) for 6 weeks, a total of 3216 differentially expressed genes (DEGs) were detected. Genes involved on biosynthesis of unsaturated fatty acids, pathogen defense, phenylalanine metabolism were obtained as key genes for increasing of storage ability of tuberous roots of sweetpotato. The results was published in Plant Physiology and Biochemistry (2017).
(3) De novo transcriptome assembly in leaves of sweetpotato was performed in leaves under low temperature stress at 4℃ (LT) and during recovery at 25℃ (RC). In comparison with non-treated controls (NT), 2,461 and 1017 differentially expressed genes (DEGs) were identified in LT and RC leaves, respectively. The detail understanding of gene regulation in response to cold stress in sweetpotato will be beneficial for future research into molecular-assisted breeding. The results was submitted to SCI Journal on October 17, 2018.

4) Function study of four genes involved in tocopherol biosynthesis in sweetpotato:
(1) Four genes [4-hydroxyphenylpyruvate dioxygenase (IbHPPD), homogentisate phytyltransferase (IbHPT), 2-methyl-6-phytylbenzoquinol methyltransferase (IbMPBQ MT), tocopherol cyclase (IbTC) and γ-tocopherol methyltransferase (IbTMT)] involved in tocopherol biosynthesis in sweetpotato were isolated and characterized. The results was published in Plant Physiology and Biochemistry (2016).
(2) Transgenic sweetpotato plants expressing IbHPPD and IbTC were generated and are under characterization.

2. Development of industrial transgenic plants with enhanced tolerance to environmental stresses
1) Development of transgenic sweetpotato plants and their characterization:
(1) Sweetpotato plants expressing IbOr gene: IbOr gene involved in accumulation of carotenoids was introduced in an orange-fleshed sweetpotato cultivar to produce both anthocyanins and carotenoids in one storage roots. The transgenic plants showed an improved tolerance to high temperature at 47℃, drought stress and oxidative stress. The results were published in Plant Physiology and Biochemistry (2015), Frontiers in Plant Science (2017) and Journal of Experimental Botany (2018).
(2) Sweetpotato plants expressing AtP3B gene: Arabidopsis AtP3B was introduced into sweetpotato. Transgenic plants showed an enhanced tolerance to both high temperature at 45℃ and low temperature at 4℃ compared to control plants.
The results was published in BMC Plant Biology (2017).
(3) Sweetpotato plants expressing IbMYB1, IbCHY-β or IbCBF3: 1) Transgenic sweetpotato plants expressing IbMYB1 gene involved in biosynthesis of anthocyannins as an transcription factor (Physiologia Plantarum, 2015), 2) Transgenic sweetpotato plants by down-regulation of β-carotene hydroxylase (IbCHY-β) by RNAi technology (Plant Physiology and Biochemistry, 2017), Transgenic sweetpotato plants expressing IbCBF3 gene involved in low temperature tolerance (Plant Physiology and Biochemistry, 2017).

2) Development of transgenic alfalfa plants and their characterization:
(1) Alfalfa plants expressing IbOr gene: IbOr gene was introduced into alfalfa (cv. Xinjang Daye), an good cultivar on marginal lands at northwest China) showed an enhanced growth and tolerance to drought stress and high salt stress compared to control plants. The results were published in PLOS One (2015).
(2) Alfalfa plants expressing AtABF3 gene: AtABF3, darought stress-tolerant gene, from Arabidopsis was introduced into alfalfa (cv. Xinjang Daye).
Transgenic plants reduced size of leaf size and showed increased tolerance to multiple abiotic stresses such as drought, high salt and oxidative stress. The results were published in Plant Physiology and Biochemistry (2016).

3) Development of transgenic poplar plants and their characterization:
(1) Poplar plants by down-regulation of PagGI genes: Thress PagGI genes from poplar plants and were down-regulated by RNAi technology. Transgenic plants showed enhanced growth and tolerance to salt stress. The results were published in Plant Biotechnology Journal (2017).
(2) Poplar plants expressing IbLEA14: IbLEA14 gene involved in drought stress tolerance in sweetpotato was introduced into poplar. Transgenic plants showed enhanced tolerance to drought, high sal and high temperature at 42℃ compared to control plants. The results were published in Environmental and Experimental Botany (2018).
(3) Poplar plants expressing AtYUCCA: Arabidopsis AtYUCCA involved in auxin biosynthesis was introduced into poplar. Transgenic plants showed anxin-overproduction phenotypes and increased enhanced tolerance to abiotic stress. The results were published in Plant Physiology and Biochemistry (2015).

3. Establishment of global research collaboration networks
1) Networks with research institutes in China
(1) To do the practical collaboration, we successfully established the strong collaboration networks with Institute of Water and Soil Conservation / Chinese Academy of Sciences (CAS) at Yangling, Sweetpotato Research Institute / Chinese Academy of Agricultural Sciences (CAAS) at Xuzhou, Sweetpotato laboratory / Jiangsh Academy of Agricultural Sciences (JAAS) at Nanjing, Guizhou University and Guizhou Academy of Agricultural Sciences (GAAS) at Guiyang etc through various activities such as exchange of reseachers and research materials, and joint symposium/workshop.
(2) In particular, four Chinese students got the Ph.D degree under the supervision of PI at KRIBB during this project and they joined at Institute of Water and Soil Conservation / CAS, Sweetpotato Research Institute /CAAS etc for sustainable collaboration in the field of agroforestry biotechnology between Korea and China.

2) Networks with research institutes in Kazakhstan
(1) To do the practical collaboration, we successfully established the strong collaboration networks with Institute of Plant Biology and Biotechnology at Almaty, National Center for Biotechnology at Astana etc. through various activities such as exchange of reseachers and research materials, and joint symposium/workshop.
(2) In particular, two young researchers visited KRIBB laboratory for collaboration researches in the field of sweetpotato biotechnology three times in 2018.

3) Networks with university in Turkey
(1) To do the practical collaboration, we successfully established the strong collaboration networks with Yeditepe University in the field sweetpotato biotechnology. Ph.D students are supposed to visit KRIBB laboratory to do collaboration researches of sweetpotato biotechnology in 2019.

4) Networks with AASSA and TRAS
(1) PI was invited to International Workshop“Climate change adaptation and mitigation: sustainable agriculture and health security” jointly organized by AASSA (Association of Academies and Societies of Sciences in Asia and FEB-RAS (Far Eastern Branch-Russian Academy of Sciences) at Birobidzhan, Russia during October 1 to 5, 2018. During the workshop, Pi could establish a good collaboration networks with participants from various countries to cope with global climate change.
(2) PI had a strong collaboration networks with sweetpotato experts by organizing the 7^th China/Japan/Korea Sweetpotato Workshop (October 10-14, 2016 at Jinan, China) and 8^th International Sweetpotato Symposium (September 5-8, 2018 at Jeonju, Korea) as an President of TRAS (Trilateral Research Association of Sweetpotato).

(출처 : SUMMARY 12p)

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 보고서 요약서(보고서 초록) ... 3
• 요 약 문 ... 4
• S U M M A R Y ... 11
• C O N T E N T S ... 17
• 목차 ... 18
• 제 1 장 연구개발과제의 개요 ... 19
• 제 2 장 국내외 기술개발 현황 ... 22
• 제 3 장 연구개발수행 내용 및 결과 ... 24
• 제1절 환경스트레스 반응 유전자 네트워크 분석 및 유용유전자 기능 분석 ... 24
• 1. 복합재해내성 IbOr 유전자의 기능분석 ... 24
• 2. 환경스트레스 관련 MAPK 스트레스 신호전달 네트워크 분석 ... 32
• 3. 저온스트레스 내성 유전자 네트워크 분석 ... 37
• 4. 고구마 토코페롤 합성 전체유전자 분리 및 기능분석 ... 44
• 제2절 환경스트레스 내성 형질전환식물 개발 및 환경재해내성 특성분석 ... 46
• 1. 형질전환(IbOr, AtP3B etc.) 고구마(5종) 개발, 환경재해내성 특성 분석 ... 46
• 2. 형질전환(IbOr, AtABF3 etc.) 알팔파(2종) 개발, 환경재해내성 특성 분석 ... 55
• 3. 형질전환(PagGI, IbLEA14 etc.) 포플러(3종) 개발, 환경재해내성 특성분석 ... 59
• 제3절 글로벌 협력 네트워크 구축 ... 65
• 1. 중국과 협력연구기반 구축 ... 65
• 2. 카자흐스탄과 협력연구기반 구축 ... 66
• 3. 터키와 협력연구기반 구축 ... 66
• 4. AASAA, TRAS와 협력기반 구축 및 강화 ... 67
• 제 4 장 목표달성도 및 관련분야에의 기여도 ... 68
• 제 5 장 연구개발결과의 활용계획 ... 72
• 제 6 장 연구개발과정에서 수집한 해외과학기술정보 ... 73
• 제 7 장 연구시설·장비 현황 ... 74
• 제 8 장 참고문헌 ... 75
• 끝페이지 ... 77