초록 ▼

연구의 목적 및 내용
인체 자궁경부 공생미생물 유전체 분석과 대사체 분석을 통한 자궁경부암 암화과정 다중 상호작용 [호스트 – 인유두종바이러스 – 공생미생물 – 환경인자] 규명
· 자궁경부 공생미생물의 community 분석과 metagenomic shotgun 분석을 통해 자궁경부 암화과정 규명
· 자궁경부암환자군과 대조군의 대사체 분석을 통해 자궁경부 암화과정 규명
· 공생미생물의 community 및 metagenomic shotgun 분석 결과와 대사체 분석 결과를 연계하여 생물정보학적 기법을 통해 자궁

연구의 목적 및 내용
인체 자궁경부 공생미생물 유전체 분석과 대사체 분석을 통한 자궁경부암 암화과정 다중 상호작용 [호스트 – 인유두종바이러스 – 공생미생물 – 환경인자] 규명
· 자궁경부 공생미생물의 community 분석과 metagenomic shotgun 분석을 통해 자궁경부 암화과정 규명
· 자궁경부암환자군과 대조군의 대사체 분석을 통해 자궁경부 암화과정 규명
· 공생미생물의 community 및 metagenomic shotgun 분석 결과와 대사체 분석 결과를 연계하여 생물정보학적 기법을 통해 자궁경부 암화과정 규명
· 호스트 – HPV – 공생미생물 – 환경인자 모두를 고려한 모델을 이용하여 생물정보학적 기법을 통해 자궁경부암 암화 과정 규명

연구개발성과
[Publication]
1. LC/MS-Based Polar Metabolite Profiling Identified Unique Biomarker Signatures for Cervical Cancer and Cervical Intraepithelial Neoplasia Using Global and Targeted Metabolomics. 2019, Cansers (Basel)
2. Dietary Inflammatory Index and Its Relationship with Cervical Carcinogenesis Risk in Korean Women: A Case-Control Study, 2019, Cansers (Basel)
3. Compositional and Functional Differences between Microbiota and Cervical Carcinogenesis as Identified by Shotgun Metagenomic Sequencing, 2019, Cansers (Basel)

[In submission]
4. A prospective analysis of cervical microbiota correlations with plasma polar metabolites in cervical carcinogenesis using a combined taxonomic and LS/MS strategy (in submission)
5. Plasma lipid profile in patients with cervical cancer and cervical intraepithelial neoplasia (in submission)
6. Taxanomic and Functional Differences in Cervical Microbiome Associated with Cervical Cancer Development (in submission)

연구개발성과의 활용계획(기대효과)
· 자궁경부 공생미생물의 연관성이 규명을 통해 자궁경부암의 새로운 예방 전략 가능.
· 자궁경부암 전 단계인 이형성증인 젊은 여성의 수 급격 증가. 자궁경부암 조기진단에 예민하지 않은 폐경 이전 여성에게 자궁경부암 전암 병변인 상피내 종양으로의 이행을 예방하는 방법 제시 가능
· 미생물을 이용한 예방 전략은 고가 백신의 혜택을 받지 못하는 계층, 젊은 여성층, 자궁경부암 발병률이 높은 중장년층이 비교적 저렴하고 안전한 방법으로 자궁경부암을 손쉽게 예방할 수 있는 경제적이고, 효과적인 방법 제시
· 암연구 기반기술 강화 및 원천기술 개발을 통해 R&D 우수성과를 창출하고 기술이전 또는 다부처 협력 대형과제 도출

(출처 : 요약문 2p)

Abstract ▼

Purpose&Contents
Investigation of cervical carcinogenesis by identifying multiple interaction of human cervical microbiome and human metabolomics using Metaomics analysis
[Host – HPV – microbiome – environment]
· Searching for biological function of cervical microbome in cervical carcinogen

Purpose&Contents
Investigation of cervical carcinogenesis by identifying multiple interaction of human cervical microbiome and human metabolomics using Metaomics analysis
[Host – HPV – microbiome – environment]
· Searching for biological function of cervical microbome in cervical carcinogenesis by community and metagenomic shotgun analysis
· Integration of Meta-Omics (metagenomic, metabolomics) data and interpretation of multiple risk factors in cervical carcinogenesis
· Pathological approach and proving of microbiome’s mechanistic role of cervical carcinogenesis using in vivo/vitro model
· Identification of cervical carcinogenesis in multiple-interaction model including host – HPV – microbiome – environment factors

Results
[Publication]
1. LC/MS-Based Polar Metabolite Profiling Identified Unique Biomarker Signatures for Cervical Cancer and Cervical Intraepithelial Neoplasia Using Global and Targeted Metabolomics. 2019, Cansers (Basel)
2. Dietary Inflammatory Index and Its Relationship with Cervical Carcinogenesis Risk in Korean Women: A Case-Control Study, 2019, Cansers (Basel)
3. Compositional and Functional Differences between Microbiota and Cervical Carcinogenesis as Identified by Shotgun Metagenomic Sequencing, 2019, Cansers (Basel)

[In submission]
4. A prospective analysis of cervical microbiota correlations with plasma polar metabolites in cervical carcinogenesis using a combined taxonomic and LS/MS strategy (in submission)
5. Plasma lipid profile in patients with cervical cancer and cervical intraepithelial neoplasia (in submission)
6. Taxanomic and Functional Differences in Cervical Microbiome Associated with Cervical Cancer Development (in submission)

Expected Contribution
· Preoccupancy of global leading group in research part for cervical microbiome
· Presenting a new prevention strategy for cervical cancer using microbiome
· Increasing of young women’s population having cervical neoplasia. Providing a preventive tip blocking the progression to cervical cancer for women not sensitive to an early diagnosis
· For class of people not having a benefit of high-price vaccine, young women, and middle-aged class, providing a cost-effective and safe way to prevent cervical carcinogenesis
· Creation of highly economic value added through the development of products for prevention of cervical carcinogenesis using microbiome

(출처 : SUMMARY 3p)

목차 Contents

• 표지 ... 1
• 국문 요약문 ... 2
• SUMMARY ... 3
• 목차 ... 4
• 1. 연구개발과제의 개요 ... 5
• 1) 연구개발의 최종목표 ... 5
• 2. 연구수행 내용 및 결과 ... 5
• 1) LC/MS-based polar metabolite profiling identified unique biomarker signatures for cervical cancer and cervical intraepithelial neoplasia using global and targeted metabolomits. 2019, Cancers (Basel) ... 5
• 2) Compositional and functional differences between microbiota and cervical carcinogenesis as identi fied by shotgun metagenomic sequencing 2019, Cancers (Basel) ... 7
• 3) Plasma lipid profile in patients with cervical cancer and cervical intraepithelial neoplasia (in submission) ... 8
• 4) Dietary Inflammatory Index and Its Relationship with Cervical Carcinogenesis Risk in Korean Women: A Case-Control Study, 2019, Cancers (Basel) ... 9
• 5) A prospective analysis of cervical microbiota correlations with plasma polar metabolites in cervical carcinogenesis using a combined taxonomic and LS/MS strategy (in submission) ... 10
• 6) Prospective investigation of metabolites-microbiome interplay in cervical carcinogenesis ... 11
• 7) Taxonomic and Functional difference in cervical microbiome associated with cervical cancer development (in submission) ... 11
• 8) Joint dietary pattern intake and plasma metabolites analysis in relation to cervical cancer development in Korean women ... 12
• 9) cervical cancer profiling using Iseq100 system을 이용한 자궁경부 미생물 분석 ... 12
• 10) 구강암 관련 수용성 대사체 프로파일링 및 바이오마커 후보물질 선별 ... 13
• 3. 연구결과의 중요성 및 활용 방안 ... 13
• 1) 연구결과의 중요성 ... 13
• 2) 연구결과의 활용방안 ... 14
• 4. 연구수행 달성도 ... 15
• 5. 연구개발과제의 대표적 연구실적 ... 15
• 6. 참여연구원 현황 ... 16
• 7. 기타사항 ... 16
• 8. 참고문헌 ... 16
• 끝페이지 ... 16