초록 ▼

1. 국산콩 생리활성성분의 항산화능 및 in vitro 세포 활성 효능 확인
2. 인체 소화액 모델과 HPLC를 이용한 이소플라본 안정성 및 변화 확인
3. In vitro 대장 모사 실험을 통한 장내미생물 균총 영향 평가 확인
4. Biotic 물질과 물리적 처리가 발아 콩의 이소플라본의 함량 증대 확인
5. 발아된 콩에서 이소플라본 추출 및 정제 공정 확립
6. 추출 및 정제된 이소플라본을 이용한 제품화, 소재화
7. 용도별 이소플라본 고·저 함유 품종 선발
8. 개화기~성숙기간 기온이 낮을

1. 국산콩 생리활성성분의 항산화능 및 in vitro 세포 활성 효능 확인
2. 인체 소화액 모델과 HPLC를 이용한 이소플라본 안정성 및 변화 확인
3. In vitro 대장 모사 실험을 통한 장내미생물 균총 영향 평가 확인
4. Biotic 물질과 물리적 처리가 발아 콩의 이소플라본의 함량 증대 확인
5. 발아된 콩에서 이소플라본 추출 및 정제 공정 확립
6. 추출 및 정제된 이소플라본을 이용한 제품화, 소재화
7. 용도별 이소플라본 고·저 함유 품종 선발
8. 개화기~성숙기간 기온이 낮을수록 이소플라본 함량의 높은 부의 상관관계 확인
9. 파종기 및 지역 등 환경요인에 따른 콩 이소플라본 함량 변이 확인

(출처 : 요약서 3p)

Abstract ▼

□ Purpose&Contents
○ Utilization and evaluation of isoflavone from Korean soybeans
- Soybeans extraction and isoflavone content analysis with various solvent
- In vitro cell viability analysis of Korean soybeans phytochemicals
- Stability evaluation of isoflaone with human digestion mode

□ Purpose&Contents
○ Utilization and evaluation of isoflavone from Korean soybeans
- Soybeans extraction and isoflavone content analysis with various solvent
- In vitro cell viability analysis of Korean soybeans phytochemicals
- Stability evaluation of isoflaone with human digestion model and HPLC
- Evaluation on gut microbiome using in vitro Simulator of the Large Intestinal Microbial Ecosystem
- Identification, characterization, antioxidant capacity, and in vitro cell viability of isoflavone-derived chemicals from Korean soybeans.
○ Development of isoflavone content enhancement technology through biotic and abiotic treatment during soybean germination
- Effects of plant growth regulators and metabolic inducers on the isoflavone contents in soybean germination
- Effects of immune activators on the isoflavone contents in soybean germination
- Effects of medicinal plant extracts on the isoflavone contents in soybean germination
- Effects of abiotic factors on the isoflavone contents in soybean germination
○ Established isoflavone extraction and purification process from germinated soybean
○ Productivity and stability improvement using extracted and purified isoflavones

Technology development
○ Continuous multi-stage extraction / purification technology improvement
- Development of isoflavone content enhancement technology of domestic soybean
- Isoflavone extraction / purification process improvement from germinated soybean

○ Materialization Technology Development
- Development of process to improve solubility / stability of purified isoflavones
- Spec decision
○ Selection of Korean soybean cultivars with high/low isoflavone content and analysis of effect of environment on isoflavone content
- to analyse the contents of total isoflavone and its components
- to study the relationship between isoflavone content and meteorological factors
- to analyse the isoflavone contents depending on planting date and locations with different latitudes

□ Results
○ Optimization of soybean extraction methods
- Three different solvents (60% MeOH, 0.1% HCl in 60% MeOH, 200 mM Tris in 60% MeOH) were tested and 60% MeOH had highest isoflavone content and malonyl isoflavone content in most of tested soybeans
○ Development of simultaneous quantitative analysis of 12 isoflavone in Korean soybeans
○ Antioxidant capacity of Korean soybeans phytochemicals
- Antioxidant capacities of Cheonja No. 3 and No. 4 were higher than others due to their anthocyanin contents
○ In vitro cell viability analysis of Korean soybeans phytochemicals
- Some of the Korean soybeans showed significant reduction in oxydative stress compared to the stress group
○ Isoflavone stability evaluation in human digestion model and HPLC
- In human digestion model, isoflavone aglycone was not degraded, but malonyl isoflavone did break down.
○ Evaluation of gut microbiome using in vitro Simulator of the Large Intestinal Microbioal Ecosystem
- Control group and the experiment group (yellow beans and black beans) showed significant difference in gut microbiome
○ Antioxidant capacity and in vitro cell viability analysis of purified isoflavone-derived chemicals
○ Isoflavone content of soybean tended to increase during the germination period.
○ The treatment of NAA, cumaric acid, chitosan and ginseng extract increased the isoflavone content in soybean germination.
○ Ultrasonic treatment before germination and light treatment during germination increased the isoflavone content in soybeangermination.
○ There was no additive effect when combining biotic substances and abiotic factors during soybean germination.
○ Isoflavone content during soybean germination was influenced not only by genotype and environmental factors, but also by the interaction effect of genotype x environmental factors.
○ Multistage extraction process improvement
- Securing isoflavone raw material, which is 4 times higher than domestic soybeans through light germination for 11 days.
- Through multi-stage extraction / purification, the yield of domestic soybean extract with more than 70% yield of isoflavones and more than 20% of isoflavones content is obtained in yield of more than 5%.
○ Materialization Technology Development
- Developed 1.6% or more water-soluble isoflavone material technology using domestic soybean and set spec for this.
○ Some soybean cultivars with high/low isoflavone content for end-uses were identified.
- Cultivars for soy-sprout were highest in isoflavone content and followed by those for soy-paste & tofu, cooking with rice, and those of early matured for cropping system and vegetables.
- Cultivars of ‘Daepung’, ‘Daepung2ho’, ‘Saegeum’ and ‘Uram’ for soy-paste & tofu and those of ‘Sowon’, ‘Sinhwa’ and ‘Sorog’ for soy-sprout had higher isoflavone content than others.
○ The lower the temperature between flowering and maturation, the higher the isoflavone content.
○ Isoflavone contents of soybean cultivars varied depending on various environments of planting dates and locations
- The more late the planting date, the more isoflavone content increased in ecological environment conditions where temperature in the period of flowering-maturity decreased.
- The variation of isoflavone and composition content considering the cultivation area and meteorological environment by latitude did not show any specific tendency.

□ Expected Contribution
○ Utilization of isoflavone from Korean soybeans in terms of stability in human digestion model and functional food in industry
○ Confirmation of specific component accumulation through biotic & abiotic treatment during seed germination may contribute to further research on seed utilization.
○ To increase the isoflavone content during soybean germination, live soybeans should be used. This will not only increase the cultivation of domestic soybeans but also increase the industrialutilization of domestic soybeans.
○ The confirmation of the accumulation of specific ingredients during the germination period will greatly contribute to the sprout vegetable business, which is currently in the spotlight as a healthfunctional food.
○ Developing a system that extracts the necessary substances in multiple stages using the whole bean seeds may help the development of small and medium-sized enterprises, which currently occupy most of the Korean soybean processing companies.
○ Commercialization of soy extract (isoflavones) to help bone health and body fat loss
○ A system that stabilizes isoflavones and increases bioavailability improves the competitiveness of isoflavones and expands the demand base to improve domestic soybean growth and distribution and the added value of farmers' activities.
○ Use of basic data to develop high value-added functional soybean cultivars
○ Increase in Korean soybean availability and improve competitiveness

(출처 : SUMMARY 7p)

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 보고서 요약서 ... 3
• 국 문 요 약 문 ... 4
• Summary ... 7
• 목차 ... 11
• 제 1 장 연구 개발 과제의 개요 ... 12
• 제1절 연구 개발 목적 ... 12
• 제2절 연구 개발의 필요성 ... 13
• 제3절 연구 개발 범위 ... 16
• 제 2 장 연구 수행 내용 및 결과 ... 18
• 제 3 장 목표달성도 및 관련분야 기여도 ... 118
• 제1절 목표대비 달성도 ... 118
• 제2절 정량적 성과 ... 121
• 제 4 장 연구 결과의 활용 계획 ... 122
• 제 5 장 연구 개발 결과의 보안 등급 ... 123
• 제 6 장 연구시설·장비종합정보시스템에 등록한 연구시설·장비 현황 ... 124
• 제 7 장 연구개발과제의 대표적 연구실적 ... 125
• 제 8 장 기타사항 ... 128
• 제 9 장 참고문헌 ... 129
• 끝페이지 ... 137