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Kafe 바로가기주관연구기관 | 한국식품연구원 Korea Food Research Institute |
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연구책임자 | 홍정선 |
참여연구자 | 최희돈 , 박동준 , 김성수 , 전기홍 , 금준석 , 김상숙 , 도정룡 , 박종대 , 김범근 , 김병목 , 최현욱 , 성정민 , 장혜원 , 최윤상 , 천용기 , 홍정선 , 이민혁 , 강민철 , 김하람 , 전준영 , 이승환 , 류아름 , 김재용 , 최은지 , 구수경 , 정민정 , 박희진 , 김현석 , 심재훈 , 임승택 , 한재준 , 백무열 , 김종예 |
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 | 한국어 |
발행년월 | 2020-12 |
과제시작연도 | 2020 |
주관부처 | 과학기술정보통신부 Ministry of Science and ICT |
등록번호 | TRKO202100000250 |
과제고유번호 | 1711123245 |
사업명 | 한국식품연구원연구운영비지원(R&D)(주요사업비) |
DB 구축일자 | 2021-05-15 |
키워드 | 공정기술.노화억제.실용화.전분구조.전분노화.구조변형.클린라벨.냉해동안정.내가공성.process technology.inhibiting retrogradation.commercializati on.starch structure.starch retrogradation.structure modification.clean label.freeze-thaw stability.process tolerance. |
(1단계)
○ 밀전분-저분자 LMP 중합체로 밀가루를 대체한 재구성 밀가루로 식빵의 노화(staling)를 지연시킴
○ Lactobacillus plantarum maltogenic amylase (LpMA) 생산조건 최적화 및 조효소액 (CELP) 처리 제빵군의 노화지연 효과 확인
○ 아밀로오스-지질 중합체 형성 및 아밀로펙틴의 재결정화 억제 최적기술 확보 및 빵과 떡(백설기, 가래떡) 적용식품의 노화지연 효과 확인
○ 전분의 물리적 변성 유도 및 분급에 의한 노화지연 효과의 기능성 밀가루 개발
○ 전분
(1단계)
○ 밀전분-저분자 LMP 중합체로 밀가루를 대체한 재구성 밀가루로 식빵의 노화(staling)를 지연시킴
○ Lactobacillus plantarum maltogenic amylase (LpMA) 생산조건 최적화 및 조효소액 (CELP) 처리 제빵군의 노화지연 효과 확인
○ 아밀로오스-지질 중합체 형성 및 아밀로펙틴의 재결정화 억제 최적기술 확보 및 빵과 떡(백설기, 가래떡) 적용식품의 노화지연 효과 확인
○ 전분의 물리적 변성 유도 및 분급에 의한 노화지연 효과의 기능성 밀가루 개발
○ 전분질 식품별 노화억제 공정기술을 활용하여 떡(27→36시간), 면(5→6개월), 빵(6→8일)의 품질유지기간 증가
○ 수분 차단성 및 미생물 부패 방지 기능성의 가식성 필름 용액의 제조 및 전분질 식품에 적용 시 노화 지연 효과 검증
○ 화학적·효소적 노화억제제를 활용하여 전분질 식품의 저장안정성 예측 모델식 개발
○ 물리적 변성 혼합처리를 통한 친환경 전분소재(clean-labelled starch)의 노화억제 효과 확인
(출처 : 요약서 4p)
(2단계)
○ 아밀로오스-지질 복합체 형성공정 및 분석법 확립, 대량생산을 통한 소재화 공정 개발
○ 천연 유화제 제조 기술 개발(대량생산 공정 포함) 및 유화안정제, 노화억제제 및 물성조절제로서의 활용 가능성 검증
○ 전분의 물성 개선을 위한 효소를 선별, 이를 활용한 변성전분 (CBAC)의 제조 및 대량 생산, 냉동생지, 소스류의 냉해동 안정화 효과 구명
○ 부분알파화 쌀소재 제조를 위한 벼의 도정 전처리 공정(습열·건열처리) 확립, 증점·내 가공성 점도·노화억제 효과 확인
○ 전분-단백질 상호작용 기반 노화지연 효과의 물리적 처리 공정 확립
○ 전분노화와 관련된 저분자 밀단백질 분해효소 생산균주를 통한 조효소액 제조, 이를 적용한 밀 소재 단백질 분해특성 및 제빵적성 확인(균주기탁: KCCM12688P)
○ 대조군 대비 냉해동 안정성 전분 소재 적용 식빵의 경도 84%, 전분-단백질 상호작용 기작 활용 머핀의 경도 63%, 부분알파화 쌀 소재 적용 냉장 주먹밥과 가래떡의 경도 각각 75%, 65% 달성
(출처 : 요약서 5p)
(Part Ⅰ)
○ Purpose & Contents
◯ Development and distribution of core technologies inhibiting starch retrogradation in starchy food
◯ Starch structure modification technology for inhibition of starch retogradation
- Retrogradation-inhibited starch materials by grafting biopolymer onto st
(Part Ⅰ)
○ Purpose & Contents
◯ Development and distribution of core technologies inhibiting starch retrogradation in starchy food
◯ Starch structure modification technology for inhibition of starch retogradation
- Retrogradation-inhibited starch materials by grafting biopolymer onto starch
- Development of bioconcersion process for starch structure modification
- Amylose-lipid complex formation and amylopectin anti-recrystallization process
- Physical modification and classification of starch materials for functional flour
◯ Applied technology for processing and application in starchy foods
- Development of retrogradation prevention process for rice cake, noodle, bread
- Development of edible coating film and restoring technology for food texture
- Development of a prediction model for storage stability of starchy foods
- Development of clean-labelled starch via physical modification
○ Results
◯ Developed a retrogradation-retarded starch-low molecular weight low-methoxy pectin (LMP) heteropolymer using protease-treated starch and LMP
◯ Established the Lactobacillus plantarum culture conditions to maximize LpMA production, obtained Lactobacillus crude enzyme(CELP) inhibiting bread staling
◯ Developed technologies for formation of amylose-lipid complex and anti-recrylstallizaton, and confirmed retardation effect on retrogradation
◯ Developed functional flour retarding bread staling via physical modification
◯ Extend shelf life of rice cake(27→36 hour) and noodle(5→6 month) through modification of production process(pretreatment, kneeding, extrusion,packaging)
◯ Confirmed antistaling effects on breads by adding hydrocolloids, modified starches and enzymes, and complimentary effects of guar gum and enzymes
◯ Investigation of antistaling effects about breads added hydrocolloids, modified starches and enzymes and complimentary effects with guar gum and enzymes.
◯ Developed functional edible film inhibiting water vapor permeability and anti-microbial effect, and confirmed antistaling effect upon food application
◯ Synergistic effects of chemicals and enzymes on starch retrogradation retardation, developed a prediction model for storage stability of starchy foods.
◯ Established clean-labelled starch processing and elucidated retrogradation inhibitory effect through its food application
○ Expected Contribution
◯ Utilize as the methodological basis for technology transfer to starch industries(starch-LMP, functional flours, clean-labelled starch, etc.)
◯ A safe(GRAS) and potential candidate(CELP) replacing imported anistaling enzymes
◯ Provide production standards for retrogradation-inhibited starchy foods
◯ Reduce food waste and enhance competitive power of food manufacturers by increasing shelf life in starchy foods
(출처 : SUMMARY 8p)
(Part II)
○ Purpose & Contents
○ Development of core technologies inhibiting starch retrogradation in starchy food, extending to functional clean materials suitable to industrial application
○ Starch structure modification technology for inhibition of starch retogradation
- Formation of amylose complex enhancing emulsifying and anti-stalling ability
- Starch nano-particle(SNP) production replacing synthetic emulsifier
- Enzymatic processing for starch with freeze-thaw stability
- Partial gelatinization process for rice utilized in refrigerated ready meal
○ Protein structure modification technology for inhibition of starch retogradation
- Complex heating process inducing anti-stalling effect of starch-protein network
- Production of anti-staling wheat flour by regulating starch-protein interaction
○ Applied technology for inhibition of starch retogradation
- Development of mass production process of amylose-lipid complex
- Process modification for starch inhibitory technology and commercialization
○ Results
○ Established production and analysis techniques for amylose complex formation varying in fatty acid chain length and in presence of debranching process
○ Developed SNP(including mass production), and confirmed functions as an emulsion stabilizer, retrogradation inhibitor, and texture modifier
○ With selection of starch modifying enzymes, a freeze-thaw stable modified starch(CBAC) was produced on a mass scale.
○ Developed wet/dry-heating process for partial-gelatinized rice grain(HRY>91%), with thickened and process-tolerant viscosity, retrogradation inhibition
○ The microwave treatment of maize starch-whey protein decreased muffin hardness to 63.4% compared to wheat flour muffins after 3 days storage
○ Established growth conditions for low-molecular wheat protein-degrading enzyme producing strains(KCCM12688P), and tested baking suitability and aging delay effect of wheat flour treated with the curde enzyme
○ Established starch-lipid complex mass production process by adjusting HMT process parameters and confirmed its stability
○ Delayed retrogradation rate of rice cake(Baekseolgi)(by 40.9%) with fermented rice flour (Lactobacillus plantarum), and bread with yudane dough
○ Retarded bread staling with freeze-thaw stable modified starch(CBAC) (84% of the control), retrogradation in refrigerated rice and rice cake(Garaedduk) with partial-gelatinized rice(75%, 65%, respectively), compared to control groups
○ Expected Contribution
○ Besides retrogradation inhibitor, enzyme-treated starch-lipid complex is possible to extend to further study encapsulating other functional substances
○ Replace synthetic emulsifiers used in processed foods with eco-friendly starch-based materials
○ Prolong quality maintenance period of freezing·refrigerating dough and sauce products by enhancing their freeze-thaw stability
○ The final product in the form of rice grain can be used as-is or further processed into flour to be utilized for various starchy food products.
○ Diversify gluten-free and anti-allergen products by integrating green technology
○ Provide economic analysis results and mass production process of anti-stalling products for breads, rice cakes, and readymeals, which can be further utilizedas basis of technology transfer.
(출처 : SUMMARY 9p)
과제명(ProjectTitle) : | - |
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연구책임자(Manager) : | - |
과제기간(DetailSeriesProject) : | - |
총연구비 (DetailSeriesProject) : | - |
키워드(keyword) : | - |
과제수행기간(LeadAgency) : | - |
연구목표(Goal) : | - |
연구내용(Abstract) : | - |
기대효과(Effect) : | - |
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