초록 ▼

자연 휴믹화 과정에서 발생하는 마이크로바이옴 변화를 평가하였고 휴믹화를 유도하는 핵심 미생물 인자를 정량하기 위한 분자 탐침자를 발굴하였으며, 이를 이용한 표준 분석프로토콜을 마련하였음. 또한 식물 테크니컬 리그닌 또는 저분자 천연 페놀 물질과 미생물 whole cell 발효 또는 효소반응을 이용하여 생물학적 방식으로 인공 휴믹화를 유도하였고, 유기물의 구조 변화와 상추/옥수수 자극 능력을 확인하였음.
일부 휴믹화 반응물에서 뿌리 자극 능력, 바이오 매스 증진, 그리고 염 저항 능력이 작물에서 향상되는 것을 관찰하였으며, 이는

자연 휴믹화 과정에서 발생하는 마이크로바이옴 변화를 평가하였고 휴믹화를 유도하는 핵심 미생물 인자를 정량하기 위한 분자 탐침자를 발굴하였으며, 이를 이용한 표준 분석프로토콜을 마련하였음. 또한 식물 테크니컬 리그닌 또는 저분자 천연 페놀 물질과 미생물 whole cell 발효 또는 효소반응을 이용하여 생물학적 방식으로 인공 휴믹화를 유도하였고, 유기물의 구조 변화와 상추/옥수수 자극 능력을 확인하였음.
일부 휴믹화 반응물에서 뿌리 자극 능력, 바이오 매스 증진, 그리고 염 저항 능력이 작물에서 향상되는 것을 관찰하였으며, 이는 생물학적 휴믹화 공정 개발이 가능함을 시사함.

(출처 : 요약서 3p)

Abstract ▼

□ Purpose&Contents
This study was conducted to develop molecular probes for quantitative analysis of key bacteria related to plant biomass humification and to establish standard protocol for precise quantitative monitoring using molecular probes. We developed molecular probe using NCBI bacterial

□ Purpose&Contents
This study was conducted to develop molecular probes for quantitative analysis of key bacteria related to plant biomass humification and to establish standard protocol for precise quantitative monitoring using molecular probes. We developed molecular probe using NCBI bacterial genome information and non-redundant DB and BLAST search about Bacillus subtilis subsp. subtilis, Bacillus velezensis, Bacillus licheniformis, Bacillus coagulence. This study also performed the identification and screening of beneficial microbial community in plant biomass decomposition-straw, pine saw dust and oak saw dust as selective biomass. The study involved micro-biome analysis in order to determine density changes of microbial ecology at different time periods (three months’ interval) during humification process.

Whole cell- or enzyme-based transformation methods were developed for upcycling of lignin and plant-derived phenols derived from paper, saccharification and food processing.
Core structural features identifiable in humic substances are also found in the fermentaed products and when lettuce and corn were treated with them, significant facilitation of root genesis, biomass increase, and salt stress resistance was recorded.

□ Results
Bacillus subtilis subsp. subtilis, Bacillus velezensis, Bacillus licheniformis, Bacillus coagulence were selected and a species or subspecies-specific probe was developed based on genomic information, and it was confirmed by PCR and Real-Time PCR.
By analyzing the density change at the species/subspecies level in various environments using these probes and real-time PCR, it was possible to track ecological variation. In order to analyze the microbiome change in the humification process according to the plant biomass type(straw, oak sawdust, pine saudust), the bacterial microbiome was analyzed after 3, 6, and 9 months. In the process, ecological variation was tracked by analyzing the density change of key bacteria.

Technical lignins from paper industries were fermentated with domestic white/brown-rot fungi, thereby producing structural variants showing enhanced 450 nm absorbance, anti-oxidant and total phenolic contents. Ultra-high resolution mass spectrometry and size-exclusion chromatography were employed to visualize their detailed molecular structures, wherein molecular components showing more oxygens and less molecular weights increased. These structural changes were accompanied with acceleration of lettuce root genesis. Fungal enzyme-based humification of small natural phenols was found to be self-assembled with apatite surfaces distributed in soils, thus resulting in enhanced phosphate release These composites (i.e., artificial humus on the surface of apatites) were also used to cultivate corns and enhanced crop productivity and salt stress resistance were induced, compared with commercial fused superphosphates. Based on these positive results, ‘Recover ingredients’ was founded in Italy to commercialize this technology as an alternative to regular chemical P fertilizers.

□ Expected Contribution
The DNA sequences for detection of the species or subspecies of the plant-biomass humification related bacteria were deposited to patent for the acquisition of intellectual property. Quantitative analysis technology can be applied to various sites and provided as basic data for the development of various products suitable for related industries.
It is expected that the technologies here we developed will be more advanced by proving their ability to hamper methanogenesis emitted from agronomic practices. In addition, it is meaningful to provide a upcycling strategy of natural phenolics that are increasingly produced from several industries as a by-product.

(출처 : SUMMARY 5p)

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 보고서 요약서 ... 3
• 국 문 요 약 문 ... 4
• Summary ... 5
• 목차 ... 6
• 제 1 장 연구 개발 과제의 개요 ... 7
• 제1절 연구 개발 목적 ... 7
• 제2절 연구 개발의 필요성 ... 7
• 제3절 연구 개발 범위 ... 15
• 제 2 장 연구 수행 내용 및 결과 ... 16
• 제1절 연구 수행 내용 ... 16
• 제2절 연구 결과 ... 21
• 제 3 장 목표달성도 및 관련분야 기여도 ... 80
• 제1절 목표대비 달성도 ... 80
• 제2절 정량적 성과(논문게재, 특허출원, 기타)를 기술 ... 81
• 제 4 장 연구 결과의 활용 계획 ... 81
• 제 5 장 연구 개발 결과의 보안 등급 ... 82
• 제 6 장 연구시설·장비종합정보시스템에 등록한 연구시설·장비 현황 ... 82
• 제 7 장 연구개발과제의 대표적 연구실적 ... 82
• 제 8 장 기타사항 ... 83
• 제 9 장 참고문헌 ... 83
• 끝페이지 ... 86