초록

1) 본 연구개발에서 조류 인플루엔자 바이러스(H5N1, H5N6, H5N8)의 HA 및 NA 항원 유전자를 여러 조합으로 바이러스 유사입자 표면에 발현하게 제조하였고 백신 항원 구성중 NA의 Heterosubtypic immunity 작용이 증명되어 범용 백신 개발 목표를 달성함.
2) 새로운 약물전달시스템이 개발되어 경구백신의 활용가능성이 보여짐에 따라 향후 본 연구결과로 사람에게 사용 가능 경구 인플루엔자 백신 개발 가능성이 보임.

(출처 : 요약서 3p)

Abstract ▼

□ Purpose&Contents
The proposal aims to develop universal avian influenza virus-like particle vaccines that are highly immunogenic and safe. Vaccines will be orally delivered and protect from various avian virus infections which would have a significant impact on avian influenzavirus infection-in

□ Purpose&Contents
The proposal aims to develop universal avian influenza virus-like particle vaccines that are highly immunogenic and safe. Vaccines will be orally delivered and protect from various avian virus infections which would have a significant impact on avian influenzavirus infection-induced economic crisis.

□ Results
< Part 1: Influenza virus-like particle vaccine against highly pathogenic avian influenza viruses >
1) Gene cloning and baculovirus generation : Genes H5, N1, N6 and N8 were obtained and rBVs were generated.
2) Virus-like particles (VLPs) vaccines below were generated. M2e5x, H5N1, H5N6, H5N8, H5N1N6, H5N1N8, H5N6N8, H5N1N6N8VLPs, H5N1N6N8 +flagellin
3) Cross-reactive VLP vaccine efficacies in mice were resulted by inducing humoral and cellular immunity, lower lung virus titers and better survivals against H5N1, H3N2 and H1N1.
4) Orally delivered influenza nanopartlce vaccines showed better protective immunity than control. Higher levels of virus-specific IgG antibody responses, CD4⁺ T cell, CD8⁺ T cells and B cells responses were detected against influenza virus (A/H1N1) infection.
5) MNT, NA activity and NAI responses by VLPs vaccination were obtained which are closely correlated with cross-protection induced by VLP vaccines in mice.

< Part 2: Development of drug delivery system of avian influenza vaccine in poultry >
1) Research on development of delivery system for oral vaccine
2) Development of a vaccine delivery system that responds to the pH
3) Nanoparticleized vaccine and validation using natural materials
4) Development of an optimized formulation that satisfies both the efficacy and economy of an oral general-purpose virus-like particle vaccine
5) Strategies for commercialization of new vaccines for veterinary drug approval
6) Development of oral vaccine delivery system applicable to poultry using natural materials

□ Expected Contribution
Various avian influenza VLPs were generated and showed cross-protection against avian and human influenza virus infections. Orally delivered nanoparticle vaccines generated in this study could protect poultry from avian virus infections which can be easily used for poultry farmers. This vaccine could significantly lower economic load induced by avian influenza virus infections.

(출처 : SUMMARY 5p)

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 보고서 요약서 ... 3
• 국 문 요 약 문 ... 4
• Summary ... 5
• 목차 ... 6
• 제 1 장 연구 개발 과제의 개요 ... 7
• 제1절 연구 개발 목적 ... 7
• 제2절 연구 개발의 필요성 ... 7
• 제3절 연구 개발 범위 ... 8
• 제 2 장 연구 수행 내용 및 결과 ... 13
• 제 3 장 목표달성도 및 관련분야 기여도 ... 31
• 제1절 목표대비 달성도 ... 31
• 제2절 정량적 성과(논문게재, 특허출원, 기타)를 기술 ... 34
• 제 4 장 연구 결과의 활용 계획 ... 34
• 제 5 장 연구 개발 결과의 보안 등급 ... 35
• 제 6 장 연구시설·장비종합정보시스템에 등록한 연구시설·장비 현황 ... 36
• 제 7 장 연구개발과제의 대표적 연구실적 ... 37
• 제 8 장 기타사항 ... 39
• 제 9 장 참고문헌 ... 41
• 끝페이지 ... 43