초록 ▼

시설작물의 주요 해충인 담배가루이, 총채벌레류, 진딧물류 등에 대한 훈증방제기술의 개발 및 약제 저항성 연구와 외국 유입해충인 유럽좁쌀바구미의 생태및 방제에 관한 연구를 수행하였다. 오이와 참외 비닐하우스에서 훈증제인 ethyl formate를 2 g/㎥의 농도로 2시간 훈증할 경우 담배가루이를 100% 방제할 수 있었고 대부분의 경우 작물에 대한 약해도 없었다. 오이총채벌레는 spinosad, 담배가루이는 chlorfenapyr에 전반적으로 높은 저항성을 가지고 있어 해당 약제는 사용에 유의해야 하며, 담배가루이 저항성 점돌연변이

시설작물의 주요 해충인 담배가루이, 총채벌레류, 진딧물류 등에 대한 훈증방제기술의 개발 및 약제 저항성 연구와 외국 유입해충인 유럽좁쌀바구미의 생태및 방제에 관한 연구를 수행하였다. 오이와 참외 비닐하우스에서 훈증제인 ethyl formate를 2 g/㎥의 농도로 2시간 훈증할 경우 담배가루이를 100% 방제할 수 있었고 대부분의 경우 작물에 대한 약해도 없었다. 오이총채벌레는 spinosad, 담배가루이는 chlorfenapyr에 전반적으로 높은 저항성을 가지고 있어 해당 약제는 사용에 유의해야 하며, 담배가루이 저항성 점돌연변이는 포화상태로 저항성 진단 기반 저항성 종합관리가 시급한 상황이었다. 유채 재배지에서 천적인 좁쌀바구미금좀벌의 활동을 강화할 수 있도록 친환경유기농자재 살포 및 재배환경을 개선하고,주변부에 개화시기가 빠른 품종의 재배, 기주식물의 저항성을 높이는 방법 등을 통해 유채에서 침입종인 유럽좁쌀바구미를 친환경적으로 관리할 수 있을 것이다.

(출처 : 요약서 3p)

Abstract ▼

□ Purpose&Contents
○ Application of fumigation with ethyl formate in green house and vinyl house, accordingly efficacy to agricultural insect pests
(Bemisia tabaci and Thrips) and phyto-toxicity to four crops
(cucumber, yellow melon, tomato and pepper) by developmental stages
○ Establish

□ Purpose&Contents
○ Application of fumigation with ethyl formate in green house and vinyl house, accordingly efficacy to agricultural insect pests
(Bemisia tabaci and Thrips) and phyto-toxicity to four crops
(cucumber, yellow melon, tomato and pepper) by developmental stages
○ Establishment of fumigant, ethyl formate treatment method for insects control in greenhouse
- Study on insecticidal efficacy and phyto-toxicity of ethyl formate fumigation
- Determiantion of optimal fumigation condition and experimental standard for pesticidal registration
○ Purpose: Development of technology for increasing efficacy of chemical control insecticides to invasive pests in greenhouse
○ Contents:
- Developing diagnostic kits for melon thrips and whitefly and monitoring
- Insecticide resistance-related genes search and regional resistance diagnosis based on molecular markers
- Screening resistance-related genes through transcriptome analysis of wetsern flower thrips and fruit fly following sublethal doseinsecticide treatment
○ This project is to confirm the spread and occurrence, to investigate the biological data in the field, to develop the suitable monitoring methods, to verify the effectiveness of organic pesticides, to detect the biological control agents, and to prepare a protocol for eco-friendly management of an invasive alien species, cabbage seedpod weevil (Ceutorhynchus obstrictus).

□ Results
○ Evaluation of uniformity and concentration of ethyl formate by fumigation times in green house and vinyl house fumigating with ethyl formate and TLV concentration of ethyl formate in post-fumigation
○ Evaluation of four agricultural crops (cucumber, yellow melon, tomato and pepper) by developmental stages in EF fumigation with different conditions of temperature, relative humidity and ethyl formate concentration by fumigation times
○ Scaled-up EF fumigation in vinyl house cultivating yellow melon and cucumber
○ This research was conducted to establish a effective management strategy for some alien insects in greenhouse and field. We tried to establish the fumigation method for control of small size pest, Bemisia tabaci etc. in greenhouse.
○ Lethal concentration*time (LCT₉₀, g·h/㎥) values in each fumigation time were 0.88 (1hr) 1.67 (2hr), 2.08 (4hr), 7.65 (12hr)for B. tabaci adult and 3.57 (1hr), 43.26 (12hr) for Frankliniella occidentalis adult.
○ In greenhouse application with designed fumigator, the fumigant efficacy against B. tabaci was 84.5% and 92.0% for 4hr (initialtreatment 1.5 g/㎥) and 2hr (1.9 g/㎥) fumigation but that for F. occidentalis was 38.6% and 1.3% lower than B. tabacci .
○ Phyto-toxicity of ethyl formate fumigation showed no damage symtoms on Cucuitacease (cucumber, yellow melon, melon, water melon, green pumpkin, smooth luffa) at all conditions.
○ We developed resistance diagnostic kits for melon thrips and whitefly and monitored resistance level against for 5 and 6 insecticides each nationwide using the kits.
○ The susceptible and resistant lines of melon thrips were sequenced, resistance-related gene list was constructed, and resistance was diagnosed nationwide through molecular markers on whitefly target genes.
○ Lists for genes up- and down-regulating related to resistance were obtained through transcriptome analysis of western flowerthrips and fruit fly following sublethal dose of insecticidestreatment.
○ We proved a point mutation on GluCl gene of western flower thrips is related with emamectin benzoate resistance.
○ The invasive alien species, cabbage seedpod weevil (CSPW) is dominating in all areas of rapeseed cultivation except Gangwon-do Province and is infesting on other cruciferous crops
(mustards), but the native species, Ceutorhynchus albosuturalis was retreated from rapeseed to cruciferous weeds. The mitogenome of CSPW is composed to 20,124 bp which includes 13protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNAs, and a single large non-coding region of 2,773 bp. On rapeseed in Suncheon which belongs to the Jeonnam Province, CSPWs were found as an overwintered adults from the early of April, damaged in seedpods by larvae from the late of April, and reached to 42.4% as the rate of damaged seedpod on May 5 in the field sown in winter season and to 56.0% on May 16 in the field sown in spring season. It is believed that the CSPW can be properly managed with an insecticide, bifenthrin WP (a.i. 2%; control effect 92.4%) in rapeseed fields for seed production and with an eco-friendly organic pesticide, pyrethrum + paraffin oil SL (a.i. 2 + 68%; control effect 59.6%) in rapeseed fields for landscape. A pteromid wasp, Chlorocytus spicatus (Walker) was identified as an ectoparasitoid on larva of CSPW, and had a parasitism of 9.52-12.82% between late May and early June in both fields of Suncheon (Jeonnam Prov.) and Andong (Gyeongbuk Prov.). A protocol for eco-friendly management of CSPW that can be used in rapeseed fields for seed production and for landscape was prepared.

□ Expected Contribution
○ Fumigation technology in the facility can be applied to future smart farm technology as a technology that can overcome future reduction of agricultural labor and concentration by comprehensively considering treatment fumigation environment
(closed and semi-closed), safety of workers, condition of ventilation post-fumigation
○ The treatment of fumigation agents in vegetable facilities is a future technology that can reduce future food production, productivity, and labor force, and can be used in current fruit andvegetable facilities, future plant factories, etc.
○ The fumigation technology to be used in glass and vinyl houses for the purpose of preventing micro-pollution in facilities is adopted and developed considering the economic and safety of existing commercial fumigation methods. It is believed that the productivity of facility cultivation can be improved by using carrier gas (CO₂)
○ The results of the above study are determined to be used as a fumigation standard and a weak evaluation standard when ethyl formates are used for melons or cucumber houses
○ Selection of optimal effective insecticides through insecticide resistance monitoring based on insecticide resistance diagnostic kit
○ Development of resistance management technology based on resistance development mechanism research results
○ Development of detoxifying enzymes-related resistance diagnostic marker
○ The invasive alien species, cabbage seedpod weevil
(Ceutorhynchus obstrictus) will be able to suppress and manage throughout biological informations, appropriate monitoring methodand a protocol for eco-friendly management in the rapeseed fieldsfor seed production and for landscape.

(출처 : SUMMARY 7p)

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 보고서 요약서 ... 3
• 국 문 요 약 문 ... 4
• Summary ... 7
• 목차 ... 11
• 제 1 장 연구 개발 과제의 개요 ... 12
• 제 1 절 연구 개발 목적 ... 12
• 제 2 절 연구 개발의 필요성 ... 12
• 제 3 절 연구 개발 범위 ... 19
• 제 2 장 연구 수행 내용 및 결과 ... 20
• 제 1 절 훈증제를 이용한 시설 내 외래미소해충 일괄 방제기술 개발 ... 20
• 제 2 절 시설재배지 내 미소해충에 대한 신규 훈증제 약효·약해 연구 ... 71
• 제 3 절 시설 내 외래 미소해충의 화학적 방제력 및 약효 증대 기술 개발 ... 93
• 제 4 절 유럽좁쌀바구미의 발육 생태 및 친환경 방제기술 개발 ... 129
• 제 3 장 목표달성도 및 관련분야 기여도 ... 169
• 제 1 절 목표대비 달성도 ... 169
• 제 2 절 정량적 성과(논문게재, 특허출원, 기타)를 기술 ... 173
• 제 4 장 연구 결과의 활용 계획 ... 175
• 제 1 절 연구개발 결과의 활용방안 ... 175
• 제 2 절 기대성과 및 파급효과 ... 176
• 제 3 절 추가적인 논문게재 ... 177
• 제 5 장 연구 개발 결과의 보안 등급 ... 178
• 제 6 장 연구시설·장비종합정보시스템에 등록한 연구시설·장비 현황 ... 179
• 제 7 장 연구개발과제의 대표적 연구실적 ... 180
• 제 8 장 기타사항 ... 182
• 제 9 장 참고문헌 ... 183
• 끝페이지 ... 194